Q89K19 (PYRB_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	blr5099

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000113107

Sequences

	Sequence		Length	Mass (Da)
	Q89K19 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: CA7975402C637239	FASTA	315	34,201
	10 20 30 40 50 60 MTSKSTFVLG HRHLLGIEGL SAADITGLLD LSEEYVELNR QVDKKRTVLR GRTQVNLFFE 70 80 90 100 110 120 ASTRTQSSFE LAGKRLGADV MNMSVSSSSI KKGETLVDTA MTLNAMHPDI LVVRHHASGA 130 140 150 160 170 180 VELLARKVDG SVINAGDGAH EHPTQALLDA LTIRRNKGRI EGLVVAICGD VLHSRVARSN 190 200 210 220 230 240 IILLNTMGAR VRVVGPSTLL PPGIERMGVE VARDMREGLN GADIVMMLRL QRERMNGSFV 250 260 270 280 290 300 PSTSEYFNYF GLDQKKLGYA KPDALVMHPG PMNRGVEIDT AVADGAQSLI REQVEMGVAV 310 RMAVLEALAR NLPNA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC50364.1.
RefSeq	NP_771739.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89K19.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1051765.
GenomeReviews	Gene locus blr5099 in contig BA000040_GR.
KEGG	bja:blr5099.
NMPDR	fig|224911.1.peg.5099.
PATRIC	21193868. VBIBraJap65052_5178.
Phylogenomic databases
HOGENOM	HBG579429.
OMA	LQRERMS.
PhylomeDB	Q89K19.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR5099-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BRAJA

Accession

Primary (citable) accession number: Q89K19

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program