Q89K08 (Q89K08_BRAJA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL BAC50376.1
Ordered Locus Names:	blr5111

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q89K08 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 34EACC60F7C3EA86	FASTA	828	91,790
	10 20 30 40 50 60 MNIVIVESPA KAKTINKYLG SSYEVLASFG HVRDLPAKNG SVDPDANFKM IWEVDPKAAG 70 80 90 100 110 120 RLNDIAKSLK NADRLILATD PDREGEAISW HVLEVLKEKR ALKDQKIERV VFNAITKQAV 130 140 150 160 170 180 TDAMKHPRQI DGALVDAYMA RRALDYLVGF TLSPVLWRKL PGARSAGRVQ SVALRLVCDR 190 200 210 220 230 240 ELEIEKFVAR EYWSLVATLL TPRGDAFEAR LVGADGKKIQ RLDIGTGAEA ENFKKALEAA 250 260 270 280 290 300 AYAVTAVDAK PARRNPQAPF TTSTLQQEAS RKYGFAPAHT MRIAQRLYEG IDIGGETTGL 310 320 330 340 350 360 ITYMRTDGVQ IDPSAITQAR KVIGEDYGNA YVPEAPRQYQ AKAKNAQEAH EAIRPTDMSR 370 380 390 400 410 420 RPDSMSRKLD SDQARLYELI WKRTIASQME AAELERTTVD ITAKAGGRTL ELRATGQVVK 430 440 450 460 470 480 FDGFLALYQE GRDDEEDEDS RRLPAMSAGD GLKRQSLAVT QHFTEPPPRF SEASLVKRME 490 500 510 520 530 540 ELGIGRPSTY ASILQVLKDR GYVKLEKKRL HGEDKGRVVV AFLESFFSRY VEYDFTANLE 550 560 570 580 590 600 EQLDRISNNE ISWQQVLKDF WNGFIGAVDE IKDLRVAQVL DVLDDMLGQH IYPPRADGGD 610 620 630 640 650 660 IRQCPSCGNG RLNLKAGKFG AFVGCSNYPE CRYTRQLAAD SEATADRSLG QDPDTGRDVW 670 680 690 700 710 720 VKAGRFGPYI QLGEQKDYEE GEKPKRAGIP KGTSPGDVEL ELALKLLSLP REIGKHPETG 730 740 750 760 770 780 QPITAGLGRF GPFVKHEKTY ASLEAGDEVF DIGLNRAVTL IAEKVAKGPS RRFGADPGKA 790 800 810 820 IGDHPTLGTV TVKSGRYGAI CHRRRRQRDD PGRVRKGHRH AAAGDRTD

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC50376.1.
RefSeq	NP_771751.1. NC_004463.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortal	Q89K08.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1051707.
GenomeReviews	Gene locus blr5111 in contig BA000040_GR.
KEGG	bja:blr5111.
NMPDR	fig|224911.1.peg.5111.
PATRIC	21193894. VBIBraJap65052_5191.
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	Q89K08.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q89K08_BRAJA

Accession

Primary (citable) accession number: Q89K08

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)