Reviewed, UniProtKB/Swiss-Prot Q89JV7 (PANC2_BRAJA)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase 2
      Short name=PS 2
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase 2
    Pantoate-activating enzyme 2

Gene names

Name:	panC2
Ordered Locus Names:	blr5162

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Pantothenate synthetase 2 HAMAP MF_00158		PRO_0000305409
Regions
	Nucleotide binding	34 – 41	8	ATP By similarity
	Nucleotide binding	152 – 155	4	ATP By similarity
	Nucleotide binding	189 – 192	4	ATP By similarity
Sites
	Active site	41	1	Proton donor By similarity
	Binding site	65	1	Beta-alanine By similarity
	Binding site	65	1	Pantoate By similarity
	Binding site	158	1	Pantoate By similarity
	Binding site	181	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89JV7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3EFFFB820731629C	FASTA	283	30,969
	10 20 30 40 50 60 MSRSPLIART VPALRRAADN LRKRKATIAL VPTMGALHDG HVSLVRLAKR RASRVVVSIF 70 80 90 100 110 120 VNPTQFAPTE DFGAYPRTWK ADIAKLAAED VDIVWHPGVE AMYPEGFATR IVPEGPALAG 130 140 150 160 170 180 LEDRFRPHFF GGVATVVGKL FTQCRPDFAI FGEKDFQQLR VVTQMARDLD LGVKVIGSRT 190 200 210 220 230 240 VRERDGLAMS SRNVYLSPQE RQTATTLYRA MKDSAGRIRA GEAIASAMAR GAATIKAAGF 250 260 270 280 VLDYFEARHA ETLAQVTSRK DGPLRILVAA KLGTTRLIDN IAV

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC50427.1.
RefSeq	NP_771802.1.
3D structure databases
HSSP	HSSP built from PDB template 1N2E based on UniProtKB P0A5R0.
SMR	Q89JV7. Positions 5-283.
ModBase	Search...
Genome annotation databases
GeneID	1051570.
GenomeReviews	Gene locus blr5162 in contig BA000040_GR.
KEGG	bja:blr5162.
NMPDR	fig|224911.1.peg.5162.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG428839.
OMA	ASSKENH.
PhylomeDB	Q89JV7.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR5162-MONOMER.
BRENDA	6.3.2.1. 280.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC2_BRAJA

Accession

Primary (citable) accession number: Q89JV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)