Q89J74 (RPOB_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	bll5410

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	1372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1372	1372	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047869

Sequences

	Sequence		Length	Mass (Da)
	Q89J74 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2F30842AC18F1B7E	FASTA	1,372	153,276
	10 20 30 40 50 60 MAQQTFTGRK RVRKFFGHIK EVAEMPNLIE VQKASYDQFL MVDEPQGGRL DEGLQAVFRS 70 80 90 100 110 120 VFPISDFSGT SMLEFVRYEF EQPKYDVDEC RQRGMTFAAP LKVTLRLIVF DIDEETGAKS 130 140 150 160 170 180 VKDIKEQDVY MGDIPLMTMN GTFIVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA 190 200 210 220 230 240 ARVIPYRGSW LDIEFDAKDI VYARIDRRRK IPVTSLMFAL GLDGEAILST FYKKILYKRT 250 260 270 280 290 300 KEGWRVPFDA NRFRGYSTIN DLIDADTGKV VLEAGKKLTV RAARQLQEKG LKALRLSDEE 310 320 330 340 350 360 LVGNYLAEDL VNPKTGEIHA EAGEEITDKN MKALNEQGYK ELPLLDIDHV NVGAYIRNTL 370 380 390 400 410 420 SADKNMTRED ALFDIYRVMR PGEPPTLDSA QAMFQSLFFD AERYDLSAVG RVKMNMRLDL 430 440 450 460 470 480 DAPDTQRTLR KEDILSVIKT LVDLRDGKGE IDDIDHLGNR RVRSVGELME NQYRIGLLRM 490 500 510 520 530 540 ERAIKERMSS VDIDTVMPQD LINAKPAAAA VREFFGSSQL SQFMDQTNPL SEITHKRRLS 550 560 570 580 590 600 ALGPGGLTRE RAGFEVRDVH PTHYGRICPI ETPEGPNIGL INSLATFARV NKYGFVETPY 610 620 630 640 650 660 RKVKDGRVTD EVVYLSAMEE GRYTVAQANV PLDPKGRFTE DLVVCRHAGE VLPVTPDKVD 670 680 690 700 710 720 YMDVSPKQLV SVAAALIPFL ENDDANRALM GSNMQRQAVP LVRAEAPFVG TGMEGVVARD 730 740 750 760 770 780 SGAAIAARRS GVIDQIDATR VVIRATEDLD PTKSGVDIYR LMKYQRSNQS TCINQRPLVK 790 800 810 820 830 840 VGDIVKKGDI IADGPSTDLG ELALGRNVLV AFMPWNGYNF EDSILLSERI VKEDVFTSIH 850 860 870 880 890 900 IEEFEVMARD TKLGPEEITR DIPNVSEEAL KNLDEAGIVY IGAEVRAGDI LVGKITPKGE 910 920 930 940 950 960 SPMTPEEKLL RAIFGEKASD VRDTSLRVPP GVQGTIVEVR VFNRHGVDKD ERALAIEREE 970 980 990 1000 1010 1020 IERLAKDRDD EQAILDRNVY NRLAELLEGR QGIAGPKGFK KDTKITRAVL EEYPKSQWWL 1030 1040 1050 1060 1070 1080 FASPNDKLMA EIEAMRKQYD ESKKGLEQRF LDKVEKLQRG DELPPGVMKM VKVFVAVKRK 1090 1100 1110 1120 1130 1140 IQPGDKMAGR HGNKGVVSKI VPIEDMPFLE DGTHADIVLN PLGVPSRMNV GQILETHLGW 1150 1160 1170 1180 1190 1200 ACAGLGKRIG QTVDAYLSKQ DIKPLKETLK KVYGEDETIK SLNDNELIEL GHNLSRGVPI 1210 1220 1230 1240 1250 1260 ATPVFDGAKE ADIEEMLKLA GLDASGQSTV YDGRTGDPFD RKVTVGYIYM LKLHHLVDDK 1270 1280 1290 1300 1310 1320 IHARSIGPYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYTLQEMLT VKSDDVAGRT 1330 1340 1350 1360 1370 KVYEAIVRGD DTFEAGIPES FNVLVKEMRS LGLNVDLHNS KMGPAPTSEA AE

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC50675.1.
RefSeq	NP_772050.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89J74.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1047500.
GenomeReviews	Gene locus bll5410 in contig BA000040_GR.
KEGG	bja:bll5410.
NMPDR	fig|224911.1.peg.5410.
PATRIC	21194512. VBIBraJap65052_5498.
Phylogenomic databases
HOGENOM	HBG285547.
OMA	QRDLTYA.
PhylomeDB	Q89J74.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL5410-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_BRAJA

Accession

Primary (citable) accession number: Q89J74

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program