Reviewed, UniProtKB/Swiss-Prot Q89HP6 (SAHH_BRAJA)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	bll5944

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000116950
Regions
	Region	224 – 389	166	NAD binding By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Binding site	231	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89HP6-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 363C0EBECD03B502	FASTA	473	52,066
	10 20 30 40 50 60 MNAKPGFTDY IVKDISLADF GRKELSLAET EMPGLMATRE EFGPKQPLKG ARIAGSLHMT 70 80 90 100 110 120 IQTGVLIETL AALGADIRWV SCNIYSTQDH AAAAIAAAGI PVFAVKGETL TEYWDYTAKL 130 140 150 160 170 180 FDWHGGGHPN MILDDGGDAT MYVHLGLRAE NGDTAFLDKP GSEEEEVFFA LLKKQLKEKP 190 200 210 220 230 240 KGYFAEIAKS IKGVSEETTT GVHRLYDMQK AGTLLWPAIN VNDSVTKSKF DNLYGCRESL 250 260 270 280 290 300 VDGIRRGTDV MMSGKVAMVA GFGDVGKGSA ASLRQAGCRV MVSEVDPICA LQAAMEGYEV 310 320 330 340 350 360 VTMEDAAPRA DIFVTATGNK DIITIEHMRA MKDRAIVCNI GHFDNEIQIA GLRNLKWTNI 370 380 390 400 410 420 KPQVDEIEFP DKHRIIMLSE GRLVNLGNAM GHPSFVMSAS FTNQTLAQIE LFANNKDGKY 430 440 450 460 470 KKEVYVLPKT LDEKVARLHL AKIGVKLTEL RKDQADYIGV KQEGPYKSDH YRY

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC51209.1.
RefSeq	NP_772584.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3R based on UniProtKB P10760.
ModBase	Search...
Genome annotation databases
GeneID	1054354.
GenomeReviews	Gene locus bll5944 in contig BA000040_GR.
KEGG	bja:bll5944.
NMPDR	fig|224911.1.peg.5944.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89HP6.
OMA	HMRAMKD.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL5944-MON.
BRENDA	3.3.1.1. 280.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_BRAJA

Accession

Primary (citable) accession number: Q89HP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)