Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q89GV2 (HUTI_BRAJA)

Last modified February 9, 2010. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase
Gene names
Name: hutI
Ordered Locus Names: bll6243
OrganismBradyrhizobium japonicum [Complete proteome] [HAMAP]
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Hide | Top

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Catalytic activity

(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372

Cofactor

Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372

Pathway

Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372

Subcellular location

Cytoplasm Potential HAMAP MF_00372.

Sequence similarities

Belongs to the hutI family.

Hide | Top

Ontologies

Keywords
   Biological processHistidine metabolism
   Cellular componentCytoplasm
   LigandIron
Metal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processhistidine catabolic process to glutamate and formamide

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionimidazolonepropionase activity

Inferred from electronic annotation. Source: HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 404404Imidazolonepropionase HAMAP MF_00372
PRO_0000306440

Sites

Metal binding731Zinc or iron By similarity
Metal binding751Zinc or iron By similarity
Metal binding2431Zinc or iron By similarity
Metal binding3181Zinc or iron By similarity
Binding site821Substrate By similarity
Binding site951Substrate By similarity
Binding site1451Substrate By similarity
Binding site1781Substrate By similarity
Binding site2461Substrate By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q89GV2-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: BC62ED8A047121EC

FASTA40443,021
        10         20         30         40         50         60 
MAERFDRIWH NARLATMRAD RPDLGEIEHG LIAARGGHIV YAGAAADFPA DADAIKRIDC 

        70         80         90        100        110        120 
AGRWITPGLV DCHTHLVYGG NRAHEFELRL KGASYEEIAR AGGGIVSTVA ATRKASEAEL 

       130        140        150        160        170        180 
VASALPRLDA LIGEGATTVE IKSGYGLDAE TEMRQLAAAR SLGRQRPVAI RTSFLGAHAL 

       190        200        210        220        230        240 
PPEADGDKDR YIDLVCKEML PAVAKAGLAD AVDTFMEGIA FSAGQTARVF ETARGLGLPV 

       250        260        270        280        290        300 
KLHADQLSNL GGAALAAKFS ALSADHLEHT DEAGAAAMAK AGTVAVLLPG AFYFIRETQK 

       310        320        330        340        350        360 
PPVESFRKHG VHMALASDCN PGSSPLTSLL LAMNMGATLF RMTVAECLAG VTREGAHALG 

       370        380        390        400 
VLDETGTLEA GKWCDLAIWD IERPAELVYR IGFNPLHRRV WRGQ

« Hide

Hide | Top

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Hide | Top

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000040 Genomic DNA. Translation: BAC51508.1.
RefSeqNP_772883.1.

3D structure databases

HSSPHSSP built from PDB template 2GOK based on UniProtKB Q8U8Z6.
SMRQ89GV2. Positions 8-404.
ModBaseSearch...

Genome annotation databases

GeneID1054564.
GenomeReviewsGene locus bll6243 in contig BA000040_GR.
KEGGbja:bll6243.
NMPDRfig|224911.1.peg.6243.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG686142.
OMAMNMACTL.

Enzyme and pathway databases

BioCycBJAP224911:BLL6243-MONOMER.
BRENDA3.5.2.7. 280.

Family and domain databases

HAMAPMF_00372. HutI.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01224. hutI. 1 hit.
ProtoNetSearch...

Hide | Top

Entry information

Entry nameHUTI_BRAJA
AccessionPrimary (citable) accession number: Q89GV2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2003
Last modified: February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents