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Protein

Nitrilase bll6402

Gene

bll6402

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Nitrilase that mediates the hydrolysis of aromatic alpha-hydroxy nitriles to carboxylic acids. Has high activity toward mandelonitrile and mediates its hydrolyzis to mandelic acid. Also mediates hydrolyzis of mandelonitrile derivatives. Has weak activity toward aliphatic nitriles.2 Publications

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.PROSITE-ProRule annotation1 Publication

Kineticsi

kcat is 27.0 sec(-1) with mandelonitrile.
  1. KM=0.26 mM for mandelonitrile1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei46Proton acceptorPROSITE-ProRule annotation1
    Active sitei128Proton donorPROSITE-ProRule annotation1
    Active sitei162NucleophilePROSITE-ProRule annotation1

    GO - Molecular functioni

    • nitrilase activity Source: UniProtKB

    GO - Biological processi

    • nitrogen compound metabolic process Source: UniProtKB
    • protein homooligomerization Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrilase bll6402 (EC:3.5.5.1)
    Alternative name(s):
    Mandelonitrile hydrolase
    Gene namesi
    Ordered Locus Names:bll6402
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    Proteomesi
    • UP000002526 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004222161 – 334Nitrilase bll6402Add BLAST334

    Interactioni

    Subunit structurei

    Homomultimer composed of 12 subunits.1 Publication

    Protein-protein interaction databases

    STRINGi224911.bll6402.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89GE3.
    SMRiQ89GE3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini6 – 284CN hydrolasePROSITE-ProRule annotationAdd BLAST279

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105ES4. Bacteria.
    COG0388. LUCA.
    HOGENOMiHOG000256364.
    InParanoidiQ89GE3.
    KOiK01502.
    OMAiWPSFSLY.
    OrthoDBiPOG091H0LO0.
    PhylomeDBiQ89GE3.

    Family and domain databases

    Gene3Di3.60.110.10. 1 hit.
    InterProiView protein in InterPro
    IPR003010. C-N_Hydrolase.
    IPR000132. Nitrilase/CN_hydratase_CS.
    PfamiView protein in Pfam
    PF00795. CN_hydrolase. 1 hit.
    SUPFAMiSSF56317. SSF56317. 1 hit.
    PROSITEiView protein in PROSITE
    PS50263. CN_HYDROLASE. 1 hit.
    PS00920. NITRIL_CHT_1. 1 hit.
    PS00921. NITRIL_CHT_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q89GE3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQDTKFKVAV VQAAPVFMDA PASVAKAIGF IAEAGAAGAK LLAFPEVWIP
    60 70 80 90 100
    GYPWWLWLGT PAWGMQFVPR YHANSLRADG PDILALCAAA AEAKINVVMG
    110 120 130 140 150
    FSEIDGGTLY LSQVFISDAG EIIFKRRKLK PTHVERTLYG EGDGSDFRVV
    160 170 180 190 200
    ESSVGRLGAL CCAEHIQPLS KYAMYSMNEQ VHVASWPSFT LYRDKAYALG
    210 220 230 240 250
    HEVNLAASQI YALEGGCFVL HASAITGQDM FDMLCDTPEK ADLLNAEGAK
    260 270 280 290 300
    PGGGYSMIFG PDGQPMCEHL PQDKEGILYA DVDLSMIAIA KAAYDPTGHY
    310 320 330
    ARGDVVRLMV NRSPRRTSVS FSEDENAAVT FTET
    Length:334
    Mass (Da):36,203
    Last modified:June 1, 2003 - v1
    Checksum:i292C9564FE840C20
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000040 Genomic DNA. Translation: BAC51667.1.
    RefSeqiNP_773042.1. NC_004463.1.
    WP_011089142.1. NZ_CP011360.1.

    Genome annotation databases

    EnsemblBacteriaiBAC51667; BAC51667; BAC51667.
    GeneIDi1047648.
    KEGGibja:bll6402.
    PATRICifig|224911.44.peg.6385.

    Similar proteinsi

    Entry informationi

    Entry nameiNITR1_BRADU
    AccessioniPrimary (citable) accession number: Q89GE3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: June 1, 2003
    Last modified: June 7, 2017
    This is version 76 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families