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Q89GA7 (DDLA_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase A

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthase A
Gene names
Name:ddlA
Ordered Locus Names:blr6438
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373D-alanine--D-alanine ligase A HAMAP-Rule MF_00047
PRO_0000177792

Regions

Domain146 – 355210ATP-grasp
Nucleotide binding179 – 23456ATP By similarity

Sites

Metal binding3081Magnesium or manganese 1 By similarity
Metal binding3221Magnesium or manganese 1 By similarity
Metal binding3221Magnesium or manganese 2 By similarity
Metal binding3241Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89GA7 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 896E99143E48A84C

FASTA37340,799
        10         20         30         40         50         60 
MADKIRVVVL YGGRSGEHEV SLKSAASVFR HLDRTRFEVI PVSIDKTGRW QWNDLRSLDQ 

        70         80         90        100        110        120 
AHAAALPILP DAPEMRLARG PDGRGVLVPI TQGAAAPIAI DVVFPVIHGP LCEDGTVQGL 

       130        140        150        160        170        180 
LELADVAYVG SGVLASAVSM DKDVAKRLAE FAGIPVAPYR VLTRKAFVQD RVSSLAKAVE 

       190        200        210        220        230        240 
GLSLPVFVKP CNMGSSVGIH KVKTQDALEA ALDDAFRYDV KVLVQQGIDA REIEVAVLED 

       250        260        270        280        290        300 
ETLFASLASE LNPNAHHEFY SYEAKYLDPD GARVDLPARL DAAQMERVRS LATRVFAALE 

       310        320        330        340        350        360 
CSGFARVDFF LDRKTGEFCF NEINTLPGFT SISMYPKMME ASGVPYGELL SRLVDLALDR 

       370 
HRQRQSLERG YAS 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC51703.1.
RefSeqNP_773078.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89GA7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr6438.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC51703; BAC51703; BAC51703.
GeneID1047518.
KEGGbja:blr6438.
PATRIC21196688. VBIBraJap65052_6578.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OrthoDBEOG64BQ73.
PhylomeDBQ89GA7.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-6486-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLA_BRADU
AccessionPrimary (citable) accession number: Q89GA7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways