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Protein

Ketol-acid reductoisomerase

Gene

ilvC

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvI)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase 2 (ilvD2), Dihydroxy-acid dehydratase 1 (ilvD1), Dihydroxy-acid dehydratase 3 (ilvD3)
  4. Branched-chain-amino-acid aminotransferase (bll7459)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvI)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase 2 (ilvD2), Dihydroxy-acid dehydratase 1 (ilvD1), Dihydroxy-acid dehydratase 3 (ilvD3)
  4. Branched-chain-amino-acid aminotransferase (bll7459)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-6545-MONOMER.
UniPathwayiUPA00047; UER00056.
UPA00049; UER00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomeraseUniRule annotation (EC:1.1.1.86UniRule annotation)
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:bll6497
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Ketol-acid reductoisomerasePRO_0000151283Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.bll6497.

Structurei

3D structure databases

ProteinModelPortaliQ89G50.
SMRiQ89G50. Positions 1-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6M. Bacteria.
COG0059. LUCA.
HOGENOMiHOG000016230.
InParanoidiQ89G50.
KOiK00053.
OrthoDBiEOG625K07.
PhylomeDBiQ89G50.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
HAMAPiMF_00435. IlvC.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR000506. AcH_isomrdctse_C.
IPR013116. IlvN.
IPR013023. Ketol-acid_reductoisomrdctse.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR21371. PTHR21371. 1 hit.
PfamiPF01450. IlvC. 1 hit.
PF07991. IlvN. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00465. ilvC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89G50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVYYDRDAD LNLIKGKKVA IVGYGSQGHA HALNLKDSGV KEVAIALRKD
60 70 80 90 100
SSSVKKAEAA GFKVMDVAEA AKWADLVMML TPDELQGDIY REHLHDNMKK
110 120 130 140 150
GAALVFAHGL NVHFNLLDPR ADLDVLMIAP KGPGHTVRSE YQRGGGVPCL
160 170 180 190 200
IAIAKDVSGN AHDLGLSYAS AVGGGRAGII ETTFKEECET DLFGEQVVLC
210 220 230 240 250
GGLVELIKGG YETLVEAGYA PEMAYFECLH EVKLIVDLIY EGGIANMNYS
260 270 280 290 300
ISNTAEYGEY VTGPRIVTAE TKAEMKRVLA DIQGGKFARD WMLENKVNQT
310 320 330
SFKATRAKLA AHPIEEVGAK LRDMMPWIKK GALVDKSKN
Length:339
Mass (Da):36,887
Last modified:June 6, 2003 - v1
Checksum:iE221BDAEA58AF8B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC51762.1.
RefSeqiNP_773137.1. NC_004463.1.
WP_011089237.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC51762; BAC51762; BAC51762.
GeneIDi1047317.
KEGGibja:bll6497.
PATRICi21196820. VBIBraJap65052_6644.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC51762.1.
RefSeqiNP_773137.1. NC_004463.1.
WP_011089237.1. NC_004463.1.

3D structure databases

ProteinModelPortaliQ89G50.
SMRiQ89G50. Positions 1-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.bll6497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC51762; BAC51762; BAC51762.
GeneIDi1047317.
KEGGibja:bll6497.
PATRICi21196820. VBIBraJap65052_6644.

Phylogenomic databases

eggNOGiENOG4105C6M. Bacteria.
COG0059. LUCA.
HOGENOMiHOG000016230.
InParanoidiQ89G50.
KOiK00053.
OrthoDBiEOG625K07.
PhylomeDBiQ89G50.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00056.
UPA00049; UER00060.
BioCyciBJAP224911:GJEJ-6545-MONOMER.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
HAMAPiMF_00435. IlvC.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR000506. AcH_isomrdctse_C.
IPR013116. IlvN.
IPR013023. Ketol-acid_reductoisomrdctse.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR21371. PTHR21371. 1 hit.
PfamiPF01450. IlvC. 1 hit.
PF07991. IlvN. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00465. ilvC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiILVC_BRADU
AccessioniPrimary (citable) accession number: Q89G50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2003
Last sequence update: June 6, 2003
Last modified: November 11, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.