Reviewed, UniProtKB/Swiss-Prot Q89G50 (ILVC_BRAJA)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	bll6497

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151283
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89G50-1 [UniParc]. Last modified June 6, 2003. Version 1. Checksum: E221BDAEA58AF8B5	FASTA	339	36,887
	10 20 30 40 50 60 MRVYYDRDAD LNLIKGKKVA IVGYGSQGHA HALNLKDSGV KEVAIALRKD SSSVKKAEAA 70 80 90 100 110 120 GFKVMDVAEA AKWADLVMML TPDELQGDIY REHLHDNMKK GAALVFAHGL NVHFNLLDPR 130 140 150 160 170 180 ADLDVLMIAP KGPGHTVRSE YQRGGGVPCL IAIAKDVSGN AHDLGLSYAS AVGGGRAGII 190 200 210 220 230 240 ETTFKEECET DLFGEQVVLC GGLVELIKGG YETLVEAGYA PEMAYFECLH EVKLIVDLIY 250 260 270 280 290 300 EGGIANMNYS ISNTAEYGEY VTGPRIVTAE TKAEMKRVLA DIQGGKFARD WMLENKVNQT 310 320 330 SFKATRAKLA AHPIEEVGAK LRDMMPWIKK GALVDKSKN

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC51762.1.
RefSeq	NP_773137.1.
3D structure databases
SMR	Q89G50. Positions 1-328.
ModBase	Search...
Genome annotation databases
GeneID	1047317.
GenomeReviews	Gene locus bll6497 in contig BA000040_GR.
KEGG	bja:bll6497.
NMPDR	fig|224911.1.peg.6497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286525.
OMA	DSISNTA.
PhylomeDB	Q89G50.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL6497-MONOMER.
BRENDA	1.1.1.86. 280.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BRAJA

Accession

Primary (citable) accession number: Q89G50

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 6, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)