Reviewed, UniProtKB/Swiss-Prot Q89G35 (TYSY_BRAJA)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	bll6512

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_0000140941
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89G35-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 112056C0A989073F	FASTA	264	29,941
	10 20 30 40 50 60 MHQYQDLLER ILSDGAEKTD RTGTGTLSVF GHQMRFNLSA GFPMLTTKRL PLKAIVHELL 70 80 90 100 110 120 WFLKGDTNIK YLRDNGVTIW DEWADANGDL GPVYGHQWRS WPAPDGRSID QIANVVDMIK 130 140 150 160 170 180 RNPDSRRLIV SAWNPAEVDK MALPPCHCLF QFYVANGKLS CQLYQRSADV FLGVPFNIAS 190 200 210 220 230 240 YALLTMMVAQ VTGLKPGDFV HSFGDTHLYS NHLEQAKLQL TRAPRALPVM RINPDVKDIF 250 260 SFRFEDFELV GYDPHPHIKA EVAV

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC51777.1.
RefSeq	NP_773152.1.
3D structure databases
HSSP	HSSP built from PDB template 1TYS based on UniProtKB P00470.
ModBase	Search...
Genome annotation databases
GeneID	1054597.
GenomeReviews	Gene locus bll6512 in contig BA000040_GR.
KEGG	bja:bll6512.
NMPDR	fig|224911.1.peg.6512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89G35.
OMA	LGPVYGH.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL6512-MON.
BRENDA	2.1.1.45. 280.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BRAJA

Accession

Primary (citable) accession number: Q89G35

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)