Reviewed, UniProtKB/Swiss-Prot Q89FY1 (ASPD_BRAJA)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	bll6567

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_0000144887
Sites
	Active site	232	1	By similarity
	Binding site	134	1	NAD; via amide nitrogen By similarity
	Binding site	202	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89FY1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 545EC9E5ACBB4F75	FASTA	280	29,472
	10 20 30 40 50 60 MDRRWKVMTD QTASSELRVA IAGLGSIGTK IAAALDQGEG LTLSAVAVRD PAKHQAFLNG 70 80 90 100 110 120 LRRPPQVLPI DQLGEAADIV VECAPSSQLR AIVEPAVKRG KAAVVVSVGG LLDNFDLVDL 130 140 150 160 170 180 ARANGGRIIV PTGALIGLDA VNAAVIGTIH SVKMVTRKPI DGLKGAPFIV HNNIDIDTLR 190 200 210 220 230 240 EPLKLFEGTA REAAKGFPAN LNVAVALSLA GVGPDRTSVQ IWADPTVTRN VHRIEVEADS 250 260 270 280 ARFSMSIENI PSENPKTGLI TALSVIALLR KQRATLCVGT

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC51832.1.
RefSeq	NP_773207.1.
3D structure databases
SMR	Q89FY1. Positions 17-278.
ModBase	Search...
Genome annotation databases
GeneID	1050893.
GenomeReviews	Gene locus bll6567 in contig BA000040_GR.
KEGG	bja:bll6567.
NMPDR	fig|224911.1.peg.6567.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649642.
OMA	FPANLNV.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL6567-MONOMER.
BRENDA	1.4.1.21. 280.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_BRAJA

Accession

Primary (citable) accession number: Q89FY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2003
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)