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Q89FY1 (ASPD_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable L-aspartate dehydrogenase

EC=1.4.1.21
Gene names
Name:nadX
Ordered Locus Names:bll6567
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length280 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP-Rule MF_01265

Catalytic activity

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP-Rule MF_01265

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP-Rule MF_01265

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.

Sequence similarities

Belongs to the L-aspartate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 280280Probable L-aspartate dehydrogenase HAMAP-Rule MF_01265
PRO_0000144887

Sites

Active site2321 By similarity
Binding site1341NAD; via amide nitrogen By similarity
Binding site2021NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89FY1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 545EC9E5ACBB4F75

FASTA28029,472
        10         20         30         40         50         60 
MDRRWKVMTD QTASSELRVA IAGLGSIGTK IAAALDQGEG LTLSAVAVRD PAKHQAFLNG 

        70         80         90        100        110        120 
LRRPPQVLPI DQLGEAADIV VECAPSSQLR AIVEPAVKRG KAAVVVSVGG LLDNFDLVDL 

       130        140        150        160        170        180 
ARANGGRIIV PTGALIGLDA VNAAVIGTIH SVKMVTRKPI DGLKGAPFIV HNNIDIDTLR 

       190        200        210        220        230        240 
EPLKLFEGTA REAAKGFPAN LNVAVALSLA GVGPDRTSVQ IWADPTVTRN VHRIEVEADS 

       250        260        270        280 
ARFSMSIENI PSENPKTGLI TALSVIALLR KQRATLCVGT 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC51832.1.
RefSeqNP_773207.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89FY1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll6567.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC51832; BAC51832; BAC51832.
GeneID1050893.
KEGGbja:bll6567.
PATRIC21196980. VBIBraJap65052_6724.

Phylogenomic databases

eggNOGCOG1712.
HOGENOMHOG000206326.
KOK06989.
OMAPSENPKT.
OrthoDBEOG6VTK2D.
ProtClustDBPRK13302.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-6617-MONOMER.
UniPathwayUPA00253; UER00456.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_01265. NadX.
InterProIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR011182. Asp_DH_NAD_syn.
IPR020626. Asp_DH_NAD_syn_prok.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFPIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNetSearch...

Entry information

Entry nameASPD_BRADU
AccessionPrimary (citable) accession number: Q89FY1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways