Reviewed, UniProtKB/Swiss-Prot Q89FU8 (MURC_BRAJA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	bll6601

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182065
Regions
	Nucleotide binding	114 – 120	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89FU8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 93CB99B69AECA407	FASTA	467	49,934
	10 20 30 40 50 60 MRLPREIGPI HFVGIGGIGM SGIAEVLVNL GYAVQGSDAS DNYNLDRLRK KGAKVSVGHK 70 80 90 100 110 120 AENVDGAEVV VVSTAIKRDN PELMAARERR IPVVRRAEML AELMRLKSCV AIAGTHGKTT 130 140 150 160 170 180 TTTMVATLLD AGGLDPTVIN GGIINAYGSN ARLGAGDWMV VEADESDGTF LKLPTDVAIV 190 200 210 220 230 240 TNVDPEHLDH FKTFEAVQDA FRHFVENLPF YGFAVMCIDH PVVQSLVGKI EDRRIITYGE 250 260 270 280 290 300 NPQADVRLVD LTPMGGGSKF KVAFRDRKTG AVHEIPDLML PMPGRHNASN ATAAIAVARE 310 320 330 340 350 360 LGVSDEAIRS AIAGFGGVKR RFTKTGEWNG VTVIDDYGHH PVEIAAVLKA ARESTNGKIV 370 380 390 400 410 420 AVVQPHRYTR LQSLFEEFCT CFNDADAVIV ADVYAAGEAP IDGIDRDHFV AGLRAHGHRE 430 440 450 460 VVPLPAAPEL AGIVKGLAKS GDLVVCLGAG NITQWAYALP GELNALG

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC51866.1.
RefSeq	NP_773241.1.
3D structure databases
SMR	Q89FU8. Positions 5-462.
ModBase	Search...
Genome annotation databases
GeneID	1052468.
GenomeReviews	Gene locus bll6601 in contig BA000040_GR.
KEGG	bja:bll6601.
NMPDR	fig|224911.1.peg.6601.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	Q89FU8.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL6601-MONOMER.
BRENDA	6.3.2.8. 280.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BRAJA

Accession

Primary (citable) accession number: Q89FU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)