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Reviewed, UniProtKB/Swiss-Prot Q89FU5 (MURD_BRAJA)

Last modified June 16, 2009. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme
Gene names
Name: murD
Ordered Locus Names: bll6604
OrganismBradyrhizobium japonicum [Complete proteome] [HAMAP]
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

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Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the murCDEF family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_0000108980

Regions

Nucleotide binding121 – 1277ATP Potential

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Sequences

Sequence LengthMass (Da)Tools
Q89FU5-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 174A447C5BB25A63

FASTA46648,501
        10         20         30         40         50         60 
MIPVTSFAGK TVAVFGLGGS GLASCHALKA GGAEVIAADD NAENVAKAAQ AGFITADLRD 

        70         80         90        100        110        120 
VSWAGFAALV LAPGVPLTHP VPHWSVLKAR EAGVEVIGDI ELFCRERRRH APDAPFVAIT 

       130        140        150        160        170        180 
GTNGKSTTTA LIAHLTKVAG YDTQMGGNIG TAILSLEPPR TGRVHVIEMS SYQIDLTPSL 

       190        200        210        220        230        240 
DPSVGILLNV SEDHIDRHGT IAHYAAVKER LVAGVQAGGT SIVGVDDGYC RDIADRLDRA 

       250        260        270        280        290        300 
GKNVVRISVK NPLASGIHVE HGTIVRTAGG ARSEVAKLGG IGSLRGLHNA QNAACAAAAA 

       310        320        330        340        350        360 
LAMGISQDVL QDGLRSFPGL AHRMEQVGRR GNVLFVNDSK GTNADATAHA LSSFADIFWI 

       370        380        390        400        410        420 
AGGKPKAGGI TSLTGFFPRI RKAYLIGEAA QEFSGTLGTQ VAHEISQTLD VAVEHAARDA 

       430        440        450        460 
EASGLTDAVV LLSPACASFD QYRNFEIRGT KFRELVQALP GVKPVV

« Hide

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References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

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Cross-references

Sequence databases

BA000040 Genomic DNA. Translation: BAC51869.1.
RefSeqNP_773244.1.

3D structure databases

HSSPHSSP built from PDB template 1E0D based on UniProtKB P14900.
ModBaseSearch...

Genome annotation databases

GeneID1051159.
GenomeReviewsGene locus bll6604 in contig BA000040_GR.
KEGGbja:bll6604.
NMPDRfig|224911.1.peg.6604.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ89FU5.
OMAQ89FU5. SEDHLDR.

Enzyme and pathway databases

BioCycBJAP224911:BLL6604-MON.
BRENDA6.3.2.9. 280.

Family and domain databases

HAMAPMF_00639.
[Tree]
InterProIPR013149. ADH_Zn-bd.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00107. ADH_zinc_N. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
TIGRFAMsTIGR01087. murD. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameMURD_BRAJA
AccessionPrimary (citable) accession number: Q89FU5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents