Reviewed, UniProtKB/Swiss-Prot Q89FU5 (MURD_BRAJA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	bll6604

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108980
Regions
	Nucleotide binding	121 – 127	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89FU5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 174A447C5BB25A63	FASTA	466	48,501
	10 20 30 40 50 60 MIPVTSFAGK TVAVFGLGGS GLASCHALKA GGAEVIAADD NAENVAKAAQ AGFITADLRD 70 80 90 100 110 120 VSWAGFAALV LAPGVPLTHP VPHWSVLKAR EAGVEVIGDI ELFCRERRRH APDAPFVAIT 130 140 150 160 170 180 GTNGKSTTTA LIAHLTKVAG YDTQMGGNIG TAILSLEPPR TGRVHVIEMS SYQIDLTPSL 190 200 210 220 230 240 DPSVGILLNV SEDHIDRHGT IAHYAAVKER LVAGVQAGGT SIVGVDDGYC RDIADRLDRA 250 260 270 280 290 300 GKNVVRISVK NPLASGIHVE HGTIVRTAGG ARSEVAKLGG IGSLRGLHNA QNAACAAAAA 310 320 330 340 350 360 LAMGISQDVL QDGLRSFPGL AHRMEQVGRR GNVLFVNDSK GTNADATAHA LSSFADIFWI 370 380 390 400 410 420 AGGKPKAGGI TSLTGFFPRI RKAYLIGEAA QEFSGTLGTQ VAHEISQTLD VAVEHAARDA 430 440 450 460 EASGLTDAVV LLSPACASFD QYRNFEIRGT KFRELVQALP GVKPVV

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC51869.1.
RefSeq	NP_773244.1.
3D structure databases
SMR	Q89FU5. Positions 6-459.
ModBase	Search...
Genome annotation databases
GeneID	1051159.
GenomeReviews	Gene locus bll6604 in contig BA000040_GR.
KEGG	bja:bll6604.
NMPDR	fig|224911.1.peg.6604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	VKYYNDS.
PhylomeDB	Q89FU5.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL6604-MONOMER.
BRENDA	6.3.2.9. 280.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR013116. IlvN. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF07991. IlvN. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BRAJA

Accession

Primary (citable) accession number: Q89FU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)