Reviewed, UniProtKB/Swiss-Prot Q89FU4 (MRAY_BRAJA)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	bll6605

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_0000108794
Regions
	Transmembrane	34 – 54	21	Potential
	Transmembrane	78 – 98	21	Potential
	Transmembrane	101 – 121	21	Potential
	Transmembrane	135 – 155	21	Potential
	Transmembrane	175 – 195	21	Potential
	Transmembrane	206 – 226	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	270 – 290	21	Potential
	Transmembrane	295 – 315	21	Potential
	Transmembrane	344 – 364	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89FU4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A176529AED38E3E4	FASTA	367	39,570
	10 20 30 40 50 60 MFYWLIELSN TFPGFGAFRT FLNVFRYITF RTGGAVVTGA LFVFLFGPWI IDHLRIRQGK 70 80 90 100 110 120 GQPIRTDGPQ SHLAKKGTPT MGGLMILSGL TVGTVLWANP LNPYVWIVLA VTLGFGFVGF 130 140 150 160 170 180 YDDYLKVTKQ TTTGFGSKLR LLIEAAIALV ACYALVRLNR DPASTALTIP FLKDTVLHFG 190 200 210 220 230 240 WFFVVFGAFV IVGAGNAVNL TDGLDGLAIV PVMIATASFA MIAYLAGNAV FADYLQIKYV 250 260 270 280 290 300 AGTGELAVLC GALLGAGLGF LWFNAPPASI FMGDTGSLAL GGMLGAIAVA VKHEIVLAVI 310 320 330 340 350 360 GGLFVLEAVS VIVQVVSFKL TGKRIFRMAP IHHHFEQLGW TEPQIVIRFW IISVMLALAG LSTLKLR

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC51870.1.
RefSeq	NP_773245.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1055053.
GenomeReviews	Gene locus bll6605 in contig BA000040_GR.
KEGG	bja:bll6605.
NMPDR	fig|224911.1.peg.6605.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89FU4.
OMA	HHHFEQK.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL6605-MON.
BRENDA	2.7.8.13. 280.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BRAJA

Accession

Primary (citable) accession number: Q89FU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)