Q89FU2 (MURE_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
EC=6.3.2.13

Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	bll6607

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium japonicum

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP-Rule MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP-Rule MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP-Rule MF_00208
Sequence similarities	Belongs to the MurCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP-Rule MF_00208		PRO_0000101870
Regions
	Nucleotide binding	106 – 112	7	ATP Potential
	Region	148 – 149	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	406 – 409	4	Meso-diaminopimelate binding By similarity
	Motif	406 – 409	4	Meso-diaminopimelate recognition motif HAMAP-Rule MF_00208
Sites
	Binding site	27	1	UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
	Binding site	29	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	175	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	181	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	183	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	382	1	Meso-diaminopimelate By similarity
	Binding site	454	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	458	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	215	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89FU2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1484B0DE1297E8A4	FASTA	485	50,491
	10 20 30 40 50 60 MKLRDLIGND AAIEPAVAAL EVTGVALDSR VVRPGDLFFA LAGSKTDGAR FIDAAVAAGA 70 80 90 100 110 120 VAIVGDHAPD GCKVPFIAVA NPRRALALAA ARFFPAQPAT IAAVTGTSGK TSVAAFTRQI 130 140 150 160 170 180 WERLGHASAS IGTIGLVSPK RTVYGSLTTP DPIALHRQMD EIAREGVTHL AFEASSHGLD 190 200 210 220 230 240 QYRLDGVRVS AGGFTNLSRD HMDYHPTVAH YLAAKLRLFR ELVPPGGAAV ISADHDCSAE 250 260 270 280 290 300 AIDAAKARGL RVMAVGRNGD GAGEGIRLTE VGVDGFSQKL TVEHRGKRYV VLLPLAGEFQ 310 320 330 340 350 360 VENALVSAGL AIGTGSDAAN VFASLEHLEG AKGRLERVGE RNGAPIFVDY AHKPDALAKA 370 380 390 400 410 420 LQALRPYAKR RLVVVFGAGG DRDAGKRPIM GEIAAENADG VIITDDNPRS EQPEAIRAAI 430 440 450 460 470 480 LATAKGAREI GDRAAAIRAA IEELEQGDAL LIAGKGHETG QIVGSEVLPF SDHEAVAAAL ASRVA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC51872.1.
RefSeq	NP_773247.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89FU2.
ModBase	Search...
Protein-protein interaction databases
STRING	224911.bll6607.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC51872; BAC51872; BAC51872.
GeneID	1051127.
KEGG	bja:bll6607.
PATRIC	21197066. VBIBraJap65052_6767.
Phylogenomic databases
eggNOG	COG0769.
HOGENOM	HOG000268118.
KO	K01928.
OMA	KLFTDHL.
ProtClustDB	PRK00139.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-6657-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
HAMAP	MF_00208. MurE.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_BRAJA

Accession

Primary (citable) accession number: Q89FU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program