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Reviewed, UniProtKB/Swiss-Prot Q89FU2 (MURE_BRAJA)

Last modified February 9, 2010. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase
Gene names
Name: murE
Ordered Locus Names: bll6607
OrganismBradyrhizobium japonicum [Complete proteome] [HAMAP]
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

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Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208

Subcellular location

Cytoplasm By similarity HAMAP MF_00208.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208

Sequence similarities

Belongs to the murCDEF family. MurE subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208
PRO_0000101870

Regions

Nucleotide binding106 – 1127ATP Potential
Region148 – 1492UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region406 – 4094Meso-diaminopimelate binding By similarity
Motif406 – 4094Meso-diaminopimelate recognition motif HAMAP MF_00208

Sites

Binding site271UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
Binding site291UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1751UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1811UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1831UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3821Meso-diaminopimelate By similarity
Binding site4541Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4581Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2151N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q89FU2-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 1484B0DE1297E8A4

FASTA48550,491
        10         20         30         40         50         60 
MKLRDLIGND AAIEPAVAAL EVTGVALDSR VVRPGDLFFA LAGSKTDGAR FIDAAVAAGA 

        70         80         90        100        110        120 
VAIVGDHAPD GCKVPFIAVA NPRRALALAA ARFFPAQPAT IAAVTGTSGK TSVAAFTRQI 

       130        140        150        160        170        180 
WERLGHASAS IGTIGLVSPK RTVYGSLTTP DPIALHRQMD EIAREGVTHL AFEASSHGLD 

       190        200        210        220        230        240 
QYRLDGVRVS AGGFTNLSRD HMDYHPTVAH YLAAKLRLFR ELVPPGGAAV ISADHDCSAE 

       250        260        270        280        290        300 
AIDAAKARGL RVMAVGRNGD GAGEGIRLTE VGVDGFSQKL TVEHRGKRYV VLLPLAGEFQ 

       310        320        330        340        350        360 
VENALVSAGL AIGTGSDAAN VFASLEHLEG AKGRLERVGE RNGAPIFVDY AHKPDALAKA 

       370        380        390        400        410        420 
LQALRPYAKR RLVVVFGAGG DRDAGKRPIM GEIAAENADG VIITDDNPRS EQPEAIRAAI 

       430        440        450        460        470        480 
LATAKGAREI GDRAAAIRAA IEELEQGDAL LIAGKGHETG QIVGSEVLPF SDHEAVAAAL 


ASRVA

« Hide

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References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000040 Genomic DNA. Translation: BAC51872.1.
RefSeqNP_773247.1.

3D structure databases

SMRQ89FU2. Positions 2-481.
ModBaseSearch...

Genome annotation databases

GeneID1051127.
GenomeReviewsGene locus bll6607 in contig BA000040_GR.
KEGGbja:bll6607.
NMPDRfig|224911.1.peg.6607.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG602753.
OMANPRSENE.
PhylomeDBQ89FU2.

Enzyme and pathway databases

BioCycBJAP224911:BLL6607-MONOMER.
BRENDA6.3.2.13. 280.

Family and domain databases

HAMAPMF_00208. MurE.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
TIGRFAMsTIGR01085. murE. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameMURE_BRAJA
AccessionPrimary (citable) accession number: Q89FU2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents