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Q89FU2 (MURE_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

EC=6.3.2.13
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene names
Name:murE
Ordered Locus Names:bll6607
OrganismBradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP-Rule MF_00208

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP-Rule MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00208

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP-Rule MF_00208

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP-Rule MF_00208
PRO_0000101870

Regions

Nucleotide binding106 – 1127ATP Potential
Region148 – 1492UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region406 – 4094Meso-diaminopimelate binding By similarity
Motif406 – 4094Meso-diaminopimelate recognition motif HAMAP-Rule MF_00208

Sites

Binding site271UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
Binding site291UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1751UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1811UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1831UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3821Meso-diaminopimelate By similarity
Binding site4541Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4581Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2151N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89FU2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 1484B0DE1297E8A4

FASTA48550,491
        10         20         30         40         50         60 
MKLRDLIGND AAIEPAVAAL EVTGVALDSR VVRPGDLFFA LAGSKTDGAR FIDAAVAAGA 

        70         80         90        100        110        120 
VAIVGDHAPD GCKVPFIAVA NPRRALALAA ARFFPAQPAT IAAVTGTSGK TSVAAFTRQI 

       130        140        150        160        170        180 
WERLGHASAS IGTIGLVSPK RTVYGSLTTP DPIALHRQMD EIAREGVTHL AFEASSHGLD 

       190        200        210        220        230        240 
QYRLDGVRVS AGGFTNLSRD HMDYHPTVAH YLAAKLRLFR ELVPPGGAAV ISADHDCSAE 

       250        260        270        280        290        300 
AIDAAKARGL RVMAVGRNGD GAGEGIRLTE VGVDGFSQKL TVEHRGKRYV VLLPLAGEFQ 

       310        320        330        340        350        360 
VENALVSAGL AIGTGSDAAN VFASLEHLEG AKGRLERVGE RNGAPIFVDY AHKPDALAKA 

       370        380        390        400        410        420 
LQALRPYAKR RLVVVFGAGG DRDAGKRPIM GEIAAENADG VIITDDNPRS EQPEAIRAAI 

       430        440        450        460        470        480 
LATAKGAREI GDRAAAIRAA IEELEQGDAL LIAGKGHETG QIVGSEVLPF SDHEAVAAAL 


ASRVA 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC51872.1.
RefSeqNP_773247.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89FU2.
ModBaseSearch...

Protein-protein interaction databases

STRING224911.bll6607.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC51872; BAC51872; BAC51872.
GeneID1051127.
KEGGbja:bll6607.
PATRIC21197066. VBIBraJap65052_6767.

Phylogenomic databases

eggNOGCOG0769.
HOGENOMHOG000268118.
KOK01928.
OMAKLFTDHL.
ProtClustDBPRK00139.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-6657-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPMF_00208. MurE.
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01085. murE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURE_BRAJA
AccessionPrimary (citable) accession number: Q89FU2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families