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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei404 – 4041NucleophileUniRule annotation
Active sitei457 – 4571Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-6819-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:blr6768
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7217211,4-alpha-glucan branching enzyme GlgBPRO_0000188688Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224911.blr6768.

Structurei

3D structure databases

ProteinModelPortaliQ89FD3.
SMRiQ89FD3. Positions 117-721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
InParanoidiQ89FD3.
KOiK00700.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ89FD3.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89FD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRAKPMPKL PAEAYAIIEG RHADPFHYLG LHPEGGKSVV RAFLPEASNV
60 70 80 90 100
EAVGEHGEVA RLDRVHDAGL FVGALPNGSK HYQLRAKFGD SIVEFEDAYR
110 120 130 140 150
FPPILTDFDL YLLGEGTHQR LYDKLGAHPM TLDGVDGIGF VVLAPNARRV
160 170 180 190 200
SVVGDFNFWD SRRHPMRVRG SGYWELFIPH AKRGDHYKFD IVGPHGHQLP
210 220 230 240 250
LKSDPMAFAS EVRPKTASIV FDEAHLPRPR PAPDGINALS APMSIYEVHL
260 270 280 290 300
GSWRRKGDNE WLTYRELAEQ LPAYARDMGF THLEFLPVSE HPFDGSWGYQ
310 320 330 340 350
PTGLYAPTSR FGTPEDFAAL VDACHREGVG VLLDWVPGHF PDDPHGLGSF
360 370 380 390 400
DGTSLYEHAN PLQGRHLDWG TLIYNYGRTE VTNFLVSNAL FWLERYAIDG
410 420 430 440 450
LRVDAVASML YLDYSRPPGA WIPNQYGGRE NIEAIAFLRR FNTELFARFP
460 470 480 490 500
QATTAAEEST AWPQVSRPVE FGGLGFGYKW NMGWMHDTLN YISKDPIHRK
510 520 530 540 550
HHHGEILFGL HYAFSENFIL PLSHDEVVHG KRSILGRMPG DEWQRFANLR
560 570 580 590 600
AYYSFMFGHP GKKLLFMGAE IAQSREWNHD QSLDWHLLEY KYHSGIQALI
610 620 630 640 650
RDLNRLYRAV PALHQMDCDQ AGFEWLITDD ANRNVFAWLR KGLDEHARCL
660 670 680 690 700
VIVNFSPNVY RNYRVRVPFA GKWKEVLNSD SAHYGGSNVG NIGEVHAVDG
710 720
KTPELRLTIP PLAAIFLVPE S
Length:721
Mass (Da):81,677
Last modified:June 1, 2003 - v1
Checksum:i41622A1E97207F87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC52033.1.
RefSeqiNP_773408.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC52033; BAC52033; BAC52033.
GeneIDi1050831.
KEGGibja:blr6768.
PATRICi21197402. VBIBraJap65052_6935.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC52033.1.
RefSeqiNP_773408.1. NC_004463.1.

3D structure databases

ProteinModelPortaliQ89FD3.
SMRiQ89FD3. Positions 117-721.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr6768.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC52033; BAC52033; BAC52033.
GeneIDi1050831.
KEGGibja:blr6768.
PATRICi21197402. VBIBraJap65052_6935.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
InParanoidiQ89FD3.
KOiK00700.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ89FD3.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciBJAP224911:GJEJ-6819-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiGLGB_BRADU
AccessioniPrimary (citable) accession number: Q89FD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.