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Q89FD3

- GLGB_BRADU

UniProt

Q89FD3 - GLGB_BRADU

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Protein
1,4-alpha-glucan branching enzyme GlgB
Gene
glgB, blr6768
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei404 – 4041Nucleophile By similarity
Active sitei457 – 4571Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-6819-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:blr6768
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7217211,4-alpha-glucan branching enzyme GlgBUniRule annotation
PRO_0000188688Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224911.blr6768.

Structurei

3D structure databases

ProteinModelPortaliQ89FD3.
SMRiQ89FD3. Positions 117-721.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ89FD3.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89FD3-1 [UniParc]FASTAAdd to Basket

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MPRAKPMPKL PAEAYAIIEG RHADPFHYLG LHPEGGKSVV RAFLPEASNV    50
EAVGEHGEVA RLDRVHDAGL FVGALPNGSK HYQLRAKFGD SIVEFEDAYR 100
FPPILTDFDL YLLGEGTHQR LYDKLGAHPM TLDGVDGIGF VVLAPNARRV 150
SVVGDFNFWD SRRHPMRVRG SGYWELFIPH AKRGDHYKFD IVGPHGHQLP 200
LKSDPMAFAS EVRPKTASIV FDEAHLPRPR PAPDGINALS APMSIYEVHL 250
GSWRRKGDNE WLTYRELAEQ LPAYARDMGF THLEFLPVSE HPFDGSWGYQ 300
PTGLYAPTSR FGTPEDFAAL VDACHREGVG VLLDWVPGHF PDDPHGLGSF 350
DGTSLYEHAN PLQGRHLDWG TLIYNYGRTE VTNFLVSNAL FWLERYAIDG 400
LRVDAVASML YLDYSRPPGA WIPNQYGGRE NIEAIAFLRR FNTELFARFP 450
QATTAAEEST AWPQVSRPVE FGGLGFGYKW NMGWMHDTLN YISKDPIHRK 500
HHHGEILFGL HYAFSENFIL PLSHDEVVHG KRSILGRMPG DEWQRFANLR 550
AYYSFMFGHP GKKLLFMGAE IAQSREWNHD QSLDWHLLEY KYHSGIQALI 600
RDLNRLYRAV PALHQMDCDQ AGFEWLITDD ANRNVFAWLR KGLDEHARCL 650
VIVNFSPNVY RNYRVRVPFA GKWKEVLNSD SAHYGGSNVG NIGEVHAVDG 700
KTPELRLTIP PLAAIFLVPE S 721
Length:721
Mass (Da):81,677
Last modified:June 1, 2003 - v1
Checksum:i41622A1E97207F87
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC52033.1.
RefSeqiNP_773408.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC52033; BAC52033; BAC52033.
GeneIDi1050831.
KEGGibja:blr6768.
PATRICi21197402. VBIBraJap65052_6935.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC52033.1 .
RefSeqi NP_773408.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89FD3.
SMRi Q89FD3. Positions 117-721.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr6768.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC52033 ; BAC52033 ; BAC52033 .
GeneIDi 1050831.
KEGGi bja:blr6768.
PATRICi 21197402. VBIBraJap65052_6935.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OrthoDBi EOG6JX7GT.
PhylomeDBi Q89FD3.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci BJAP224911:GJEJ-6819-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiGLGB_BRADU
AccessioniPrimary (citable) accession number: Q89FD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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