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Q89EN5 (NAPA_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:blr7038
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 837805Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045979

Regions

Domain44 – 100574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding581Iron-sulfur (4Fe-4S) By similarity
Metal binding861Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict2821K → N in AAG31648. Ref.2
Sequence conflict307 – 3082GL → RV in AAG31648. Ref.2
Sequence conflict326 – 3316DSTDMS → RNDPRQD Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q89EN5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 0F5976FF14FDD5CE

FASTA83794,215
        10         20         30         40         50         60 
MTSPKLDRRQ MLKLEAAAIA AAAAGLPVPA LAANLATERE VSELKWDKAA CRFCGTGCSV 

        70         80         90        100        110        120 
MVATKENRVV ATHGDIKAEV NRGLNCVKGY FLSKIMYGHD RLTQPMLRKA NGKYDKNGDF 

       130        140        150        160        170        180 
TPVSWTEAFD IMEVKWKEAM KKRGPNGVAM FGSGQWTIWE GYAASKLFKA GFRTNNIDPN 

       190        200        210        220        230        240 
ARHCMASAVA GMMRTFGIDE PPGCYDDIEA TDAFVLWGSN MAEMHPILWT RLTDRRLSAP 

       250        260        270        280        290        300 
HVRVAVLSTF EHRSFDLADI GMVFKPQTDL YLLNAIANHI IKTGRVNKDF VAAHTVFRRG 

       310        320        330        340        350        360 
QTDIGYGLRP EHPLQKKATG AAKANDSTDM SYDEYVKFVS EYTLEKAAEM SGVPLNRLEA 

       370        380        390        400        410        420 
LAELYADPKT KVVSFWTMGF NQHTRGVWCN NLVYNIHLLT GKISSPGNSP FSLTGQPSAC 

       430        440        450        460        470        480 
GTAREVGTFS HRLPADMVVT NKEHRTKAEH IWQLPEGTIP DKPGAHAVLQ SRMLKDGLIN 

       490        500        510        520        530        540 
AYWVQVNNNL QAGPNANEET YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA 

       550        560        570        580        590        600 
YGNAERRTQF WHQLVPAPGE SKSDLWQLME FSKRFKIEEV WPEELIAKKP EVRGKTLFDV 

       610        620        630        640        650        660 
LYKNGQVDKF PVSDIEQGYL NDESKAFGFY VHKGLFEEYA SFGRGHGHDL APFDAYHKER 

       670        680        690        700        710        720 
GLRWPVVNGQ ETRWRFREGS DPYVKQGTDV QFYGYPDGKA RIFALPYEPA AESPDGEYPF 

       730        740        750        760        770        780 
WLSTGRVLEH WHSGTMTRRV PELYKAFPEA VCFMHPDDAQ EAKIRRGDEV KVVSRRGFIR 

       790        800        810        820        830 
VRVETRGRDR PPRGLVFVPW FDESKLINKV TLDATDPISL QTDFKKCAVR IERVNVS 

« Hide

References

« Hide 'large scale' references
[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.
[2]"The Bradyrhizobium japonicum napEDABC genes encoding the periplasmic nitrate reductase are essential for nitrate respiration."
Delgado M., Bonnard N., Tresierra-Ayala A., Bedmar E.J., Muller P.
Microbiology 149:3395-3403(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: USDA 110spc4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC52303.1.
AF314590 Genomic DNA. Translation: AAG31648.2.
RefSeqNP_773678.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89EN5.
SMRQ89EN5. Positions 44-832.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr7038.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC52303; BAC52303; BAC52303.
GeneID1048006.
KEGGbja:blr7038.
PATRIC21197969. VBIBraJap65052_7217.

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFHAVLQD.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-7091-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_BRADU
AccessionPrimary (citable) accession number: Q89EN5
Secondary accession number(s): Q9F5L3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families