Reviewed, UniProtKB/Swiss-Prot Q89DR8 (CARA_BRAJA)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	blr7371

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112258
Regions
	Domain	208 – 396	189	Glutamine amidotransferase type-1
	Region	1 – 204	204	CPSase HAMAP MF_01209
Sites
	Active site	285	1	Nucleophile By similarity
	Active site	369	1	By similarity
	Active site	371	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89DR8-1 [UniParc]. Last modified June 6, 2003. Version 1. Checksum: 972E66B77EEE23A4	FASTA	396	42,714
	10 20 30 40 50 60 MTQHDNDPAW PDHKPTALLV LADGTVLEGF GLGAEGHAVG EVCFNTAMTG YEEILTDPSY 70 80 90 100 110 120 AGQLITFTFP HIGNVGTNEE DIETVNMAAT PGARGVILRT AITDPSNYRA TKHLDAWLKA 130 140 150 160 170 180 RGIIGLSGID TRALTALIRS KGMPNAVIAH ARNGEFDLHG LKEEAREWPG LEGMDLVPMV 190 200 210 220 230 240 TSGQRFTWDE TPWLWDKGFG QQDKTEFNVV AIDYGIKRNI LRLLAGVGCK VTVVPATTSA 250 260 270 280 290 300 EDILAMKPDG VFLSNGPGDP AATGKYAVPV IRDVIKSGTP TFGICLGHQM LGLAVGAKTK 310 320 330 340 350 360 KMHQGHHGAN HPVKDETTGK VEITSMNHGF AVDEKTLPKG ATQTHISLFD GSNCGIQLDG 370 380 390 KPVFSVQYHP EASPGPRDSH YLFQRFAELM RQKKSA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC52636.1.
RefSeq	NP_774011.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1054607.
GenomeReviews	Gene locus blr7371 in contig BA000040_GR.
KEGG	bja:blr7371.
NMPDR	fig|224911.1.peg.7371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89DR8.
OMA	Q89DR8. LFDGSNC.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR7371-MON.
BRENDA	6.3.5.5. 280.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_BRAJA

Accession

Primary (citable) accession number: Q89DR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 6, 2003
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)