Reviewed, UniProtKB/Swiss-Prot Q89DJ1 (KPRS_BRAJA)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	blr7448

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141115
Regions
	Region	216 – 229	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	132	1	Magnesium Potential
	Metal binding	134	1	Magnesium Potential
	Metal binding	143	1	Magnesium Potential
	Metal binding	147	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89DJ1-1 [UniParc]. Last modified July 3, 2003. Version 2. Checksum: F611A9C440B5EF25	FASTA	317	33,868
	10 20 30 40 50 60 MSAKNGSIKL VAGNSNPALA QAIAQGLHLP LTKAVVRRFA DMEIFVEIQE NVRGSDAFII 70 80 90 100 110 120 QSTSFPANDH LMELLIITDA LRRSSARRIT AVLPYFGYAR QDRKSGSRTP ISAKLVANLI 130 140 150 160 170 180 TQAGVDRVMT LDLHAGQIQG FFDIPTDNLY AAPLMVRDIK DKFDLSKTMV ISPDVGGVAR 190 200 210 220 230 240 ARGLAKRINT PLAIVDKRRE RPGESEVMNV IGDVAGYTCI LVDDIVDSGG TLVNAADALI 250 260 270 280 290 300 AKGAKDVYAY ITHGVLSGGA AARITNSKLK ELVITDSILP TDAVSKAPNI RTLPIASLIS 310 DAIARTAAEE SVSSLFD

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC52713.1. Different initiation.
RefSeq	NP_774088.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKU based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	1047268.
GenomeReviews	Gene locus blr7448 in contig BA000040_GR.
KEGG	bja:blr7448.
NMPDR	fig|224911.1.peg.7448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89DJ1.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR7448-MON.
BRENDA	2.7.6.1. 280.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_BRAJA

Accession

Primary (citable) accession number: Q89DJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	July 3, 2003
Last modified:	September 22, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)