Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribose-phosphate pyrophosphokinase

Gene

prs

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of ribose 1,5-bisphosphate. Catalyzes the transfer of pyrophosphoryl group from ATP to ribose-5-phosphate to yield phosphoribosyl diphosphate (PRPP) and AMP.UniRule annotation

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase (prs)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei108 – 1081Ribose-5-phosphateUniRule annotation
Metal bindingi132 – 1321MagnesiumUniRule annotation
Metal bindingi134 – 1341MagnesiumUniRule annotation
Binding sitei134 – 1341ATPUniRule annotation
Binding sitei139 – 1391ATPUniRule annotation
Metal bindingi143 – 1431MagnesiumUniRule annotation
Metal bindingi147 – 1471MagnesiumUniRule annotation
Binding sitei174 – 1741Ribose-5-phosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 433ATPUniRule annotation
Nucleotide bindingi100 – 1034ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-7503-MONOMER.
UniPathwayiUPA00087; UER00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinaseUniRule annotation (EC:2.7.6.1UniRule annotation)
Short name:
RPPKUniRule annotation
Alternative name(s):
5-phospho-D-ribosyl alpha-1-diphosphateUniRule annotation
Phosphoribosyl diphosphate synthaseUniRule annotation
Phosphoribosyl pyrophosphate synthaseUniRule annotation
Short name:
P-Rib-PP synthaseUniRule annotation
Short name:
PRPP synthaseUniRule annotation
Short name:
PRPPaseUniRule annotation
Gene namesi
Name:prsUniRule annotation
Synonyms:prsA
Ordered Locus Names:blr7448
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Ribose-phosphate pyrophosphokinasePRO_0000141115Add
BLAST

Proteomic databases

PRIDEiQ89DJ1.

Interactioni

Protein-protein interaction databases

STRINGi224911.blr7448.

Structurei

3D structure databases

ProteinModelPortaliQ89DJ1.
SMRiQ89DJ1. Positions 7-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 1993Ribose-5-phosphate bindingUniRule annotation
Regioni224 – 2318Ribose-5-phosphate bindingUniRule annotation
Regioni311 – 3133Ribose-5-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the ribose-phosphate pyrophosphokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C5T. Bacteria.
COG0462. LUCA.
HOGENOMiHOG000210449.
InParanoidiQ89DJ1.
KOiK00948.
OMAiHENVRGQ.
OrthoDBiPOG091H018X.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_B. RibP_PPkinase_B. 1 hit.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89DJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKNGSIKL VAGNSNPALA QAIAQGLHLP LTKAVVRRFA DMEIFVEIQE
60 70 80 90 100
NVRGSDAFII QSTSFPANDH LMELLIITDA LRRSSARRIT AVLPYFGYAR
110 120 130 140 150
QDRKSGSRTP ISAKLVANLI TQAGVDRVMT LDLHAGQIQG FFDIPTDNLY
160 170 180 190 200
AAPLMVRDIK DKFDLSKTMV ISPDVGGVAR ARGLAKRINT PLAIVDKRRE
210 220 230 240 250
RPGESEVMNV IGDVAGYTCI LVDDIVDSGG TLVNAADALI AKGAKDVYAY
260 270 280 290 300
ITHGVLSGGA AARITNSKLK ELVITDSILP TDAVSKAPNI RTLPIASLIS
310
DAIARTAAEE SVSSLFD
Length:317
Mass (Da):33,868
Last modified:July 3, 2003 - v2
Checksum:iF611A9C440B5EF25
GO

Sequence cautioni

The sequence BAC52713 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC52713.1. Different initiation.
RefSeqiNP_774088.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC52713; BAC52713; BAC52713.
GeneIDi1047268.
KEGGibja:blr7448.
PATRICi21198875. VBIBraJap65052_7667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC52713.1. Different initiation.
RefSeqiNP_774088.1. NC_004463.1.

3D structure databases

ProteinModelPortaliQ89DJ1.
SMRiQ89DJ1. Positions 7-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr7448.

Proteomic databases

PRIDEiQ89DJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC52713; BAC52713; BAC52713.
GeneIDi1047268.
KEGGibja:blr7448.
PATRICi21198875. VBIBraJap65052_7667.

Phylogenomic databases

eggNOGiENOG4105C5T. Bacteria.
COG0462. LUCA.
HOGENOMiHOG000210449.
InParanoidiQ89DJ1.
KOiK00948.
OMAiHENVRGQ.
OrthoDBiPOG091H018X.

Enzyme and pathway databases

UniPathwayiUPA00087; UER00172.
BioCyciBJAP224911:GJEJ-7503-MONOMER.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_B. RibP_PPkinase_B. 1 hit.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPRS_BRADU
AccessioniPrimary (citable) accession number: Q89DJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: July 3, 2003
Last modified: September 7, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.