Reviewed, UniProtKB/Swiss-Prot Q89DI7 (RISB2_BRAJA)

Names and origin

Protein names

Recommended name:
    6,7-dimethyl-8-ribityllumazine synthase 2
      Short name=DMRL synthase 2
      Short name=Lumazine synthase 2
    EC=2.5.1.9
Alternative name(s):
    Riboflavin synthase 2 beta chain

Gene names

Name:	ribH2
Ordered Locus Names:	bll7452

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	6,7-dimethyl-8-ribityllumazine synthase 2 HAMAP MF_00178		PRO_0000134723

Sequences

	Sequence		Length	Mass (Da)
	Q89DI7-1 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 1D7D17F08C482C8C	FASTA	165	18,539
	10 20 30 40 50 60 MDPPAIEHPR FAKPQRVAFV QACWHRDVVE EARIAFMKEA EARHLTHVDV FEVPGSFEIP 70 80 90 100 110 120 LHAQILAKTR RYTAIVAAGL VVDGGIYRHE FVADTVIKAL MDVQLRTEVP VFSAVLTPQQ 130 140 150 160 FHETEVHYDF FRRHFAIKGV EVAEACANTL LGLERLRGQV AAGIA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC52717.1. Different initiation.
RefSeq	NP_774092.1.
3D structure databases
HSSP	HSSP built from PDB template 1DI0 based on UniProtKB Q44668.
ModBase	Search...
Genome annotation databases
GeneID	1047922.
GenomeReviews	Gene locus bll7452 in contig BA000040_GR.
KEGG	bja:bll7452.
NMPDR	fig|224911.1.peg.7452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89DI7.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL7452-MON.
BRENDA	2.5.1.9. 280.
Family and domain databases
HAMAP	MF_00178. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
ProDom	PD003664. DMRL_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RISB2_BRAJA

Accession

Primary (citable) accession number: Q89DI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	September 22, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)