Q89DI7 (RISB2_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase 2
Short name=DMRL synthase 2
Short name=Lumazine synthase 2
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase 2 beta chain

Gene names

Name:	ribH2
Ordered Locus Names:	bll7452

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.
Sequence caution	The sequence BAC52717.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	6,7-dimethyl-8-ribityllumazine synthase 2 HAMAP MF_00178		PRO_0000134723

Sequences

	Sequence		Length	Mass (Da)
	Q89DI7 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 1D7D17F08C482C8C	FASTA	165	18,539
	10 20 30 40 50 60 MDPPAIEHPR FAKPQRVAFV QACWHRDVVE EARIAFMKEA EARHLTHVDV FEVPGSFEIP 70 80 90 100 110 120 LHAQILAKTR RYTAIVAAGL VVDGGIYRHE FVADTVIKAL MDVQLRTEVP VFSAVLTPQQ 130 140 150 160 FHETEVHYDF FRRHFAIKGV EVAEACANTL LGLERLRGQV AAGIA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC52717.1. Different initiation.
RefSeq	NP_774092.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89DI7.
SMR	Q89DI7. Positions 15-156.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1047922.
GenomeReviews	Gene locus bll7452 in contig BA000040_GR.
KEGG	bja:bll7452.
NMPDR	fig|224911.1.peg.7452.
PATRIC	21198881. VBIBraJap65052_7670.
Phylogenomic databases
HOGENOM	HBG311126.
OMA	RRYTAIV.
PhylomeDB	Q89DI7.
ProtClustDB	PRK12419.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL7452-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB2_BRAJA

Accession

Primary (citable) accession number: Q89DI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program