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Q89DF8

- SYI_BRADU

UniProt

Q89DF8 - SYI_BRADU

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Protein

Isoleucine--tRNA ligase

Gene
ileS, blr7481
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301Aminoacyl-adenylate By similarity
Binding sitei674 – 6741ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-7536-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:blr7481
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Isoleucine--tRNA ligaseUniRule annotationPRO_0000098360Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224911.blr7481.

Structurei

3D structure databases

ProteinModelPortaliQ89DF8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 8011"HIGH" regionUniRule annotationAdd
BLAST
Motifi671 – 6755"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OrthoDBiEOG644ZM1.
PhylomeDBiQ89DF8.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89DF8-1 [UniParc]FASTAAdd to Basket

« Hide

MSEKPQKSQK SEVKDYSKTL FLPQTEFPMR AGLPQREPEI LKYWNDIGLY     50
DRLRQEAEGR TKFVLHDGPP YANGNIHIGH ALNKILKDVV TKSQQMLGFD 100
SNYVPGWDCH GLPIEWKIEE ENYRKKGKQK PDFRDSAAMV AFRKECRAYA 150
THWINVQREE FKRLGIIGDW DHPYQTMSYP AEAQIARELM KFAANGTLYR 200
GSKPVMWSVV EKTALAEAEV EYEDYTSDMV WVKFPVTSPA HGALASASVV 250
IWTTTPWTLP GNRAISFSPK IAYGLYKVTD APADNWAKTG DLLILADALA 300
AEVFKQARVT TYEKVRELPA DTLDAVECAH PLKGFSGGYE FTVPLLPGDH 350
VTDDTGTGFV HTAPGHGRED FDVWMANARE LEARGINTTI PYTVDENGAF 400
TDHAPGFVGK RVINDKGEKG DANEAVIKAL IDAGKLLARG RLKHQYPHSW 450
RSKKPVIFRN TPQWFIAMDK DISANGHAKK GDTLRARALH AISVTQWVPP 500
SGENRINGMI ANRPDWVISR QRAWGVPIAV FVREKSDGSA EILQDEIVNQ 550
RIAEAFMEEG ADAWYMDGAR ERFLGSRASE DWKKVDDICD VWFDSGSTHA 600
FVLEDRQNFP QLGNIVRKVD GGSDTVMYLE GSDQHRGWFH SSLLESAGTR 650
GRAPYDIVLT HGFTLDENGR KMSKSLGNTV EPQKVIKDSG ADILRLWVCA 700
TDYADDQRIG PEILKNTIET YRKLRNSIRW MLGTLHHFKP SEKVAYAEMP 750
ELERLMLHEL AGHAETVRNA YAAFDYKTVV ASLAAFMNSE LSAFYFDIRK 800
DTLYCDPPSS PARKAALTTI DLLCDAILKW LAPILSFTTD EAWRMFRPNA 850
EPSVHLTLFP ADIEKLRDDK LAAKWETIRN VRRAVTGALE LERAAKNIGS 900
SLEASPVIYV ADRDMLATLF DTDLAEVCIT SNYEVRESEA PASAFRLDAV 950
PGVAVVVEKA VGTKCARSWK ISQTVGEDPE YPDVTPRDAQ ALREWKALGV 1000
GV 1002
Length:1,002
Mass (Da):112,383
Last modified:June 1, 2003 - v1
Checksum:i41ABFF403E52F837
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC52746.1.
RefSeqiNP_774121.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC52746; BAC52746; BAC52746.
GeneIDi1054266.
KEGGibja:blr7481.
PATRICi21198941. VBIBraJap65052_7700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC52746.1 .
RefSeqi NP_774121.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89DF8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr7481.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC52746 ; BAC52746 ; BAC52746 .
GeneIDi 1054266.
KEGGi bja:blr7481.
PATRICi 21198941. VBIBraJap65052_7700.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OrthoDBi EOG644ZM1.
PhylomeDBi Q89DF8.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-7536-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiSYI_BRADU
AccessioniPrimary (citable) accession number: Q89DF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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