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Q89DF8

- SYI_BRADU

UniProt

Q89DF8 - SYI_BRADU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei630 – 6301Aminoacyl-adenylateUniRule annotation
    Binding sitei674 – 6741ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-7536-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:blr7481
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10021002Isoleucine--tRNA ligasePRO_0000098360Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224911.blr7481.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89DF8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi70 – 8011"HIGH" regionAdd
    BLAST
    Motifi671 – 6755"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OrthoDBiEOG644ZM1.
    PhylomeDBiQ89DF8.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q89DF8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEKPQKSQK SEVKDYSKTL FLPQTEFPMR AGLPQREPEI LKYWNDIGLY     50
    DRLRQEAEGR TKFVLHDGPP YANGNIHIGH ALNKILKDVV TKSQQMLGFD 100
    SNYVPGWDCH GLPIEWKIEE ENYRKKGKQK PDFRDSAAMV AFRKECRAYA 150
    THWINVQREE FKRLGIIGDW DHPYQTMSYP AEAQIARELM KFAANGTLYR 200
    GSKPVMWSVV EKTALAEAEV EYEDYTSDMV WVKFPVTSPA HGALASASVV 250
    IWTTTPWTLP GNRAISFSPK IAYGLYKVTD APADNWAKTG DLLILADALA 300
    AEVFKQARVT TYEKVRELPA DTLDAVECAH PLKGFSGGYE FTVPLLPGDH 350
    VTDDTGTGFV HTAPGHGRED FDVWMANARE LEARGINTTI PYTVDENGAF 400
    TDHAPGFVGK RVINDKGEKG DANEAVIKAL IDAGKLLARG RLKHQYPHSW 450
    RSKKPVIFRN TPQWFIAMDK DISANGHAKK GDTLRARALH AISVTQWVPP 500
    SGENRINGMI ANRPDWVISR QRAWGVPIAV FVREKSDGSA EILQDEIVNQ 550
    RIAEAFMEEG ADAWYMDGAR ERFLGSRASE DWKKVDDICD VWFDSGSTHA 600
    FVLEDRQNFP QLGNIVRKVD GGSDTVMYLE GSDQHRGWFH SSLLESAGTR 650
    GRAPYDIVLT HGFTLDENGR KMSKSLGNTV EPQKVIKDSG ADILRLWVCA 700
    TDYADDQRIG PEILKNTIET YRKLRNSIRW MLGTLHHFKP SEKVAYAEMP 750
    ELERLMLHEL AGHAETVRNA YAAFDYKTVV ASLAAFMNSE LSAFYFDIRK 800
    DTLYCDPPSS PARKAALTTI DLLCDAILKW LAPILSFTTD EAWRMFRPNA 850
    EPSVHLTLFP ADIEKLRDDK LAAKWETIRN VRRAVTGALE LERAAKNIGS 900
    SLEASPVIYV ADRDMLATLF DTDLAEVCIT SNYEVRESEA PASAFRLDAV 950
    PGVAVVVEKA VGTKCARSWK ISQTVGEDPE YPDVTPRDAQ ALREWKALGV 1000
    GV 1002
    Length:1,002
    Mass (Da):112,383
    Last modified:June 1, 2003 - v1
    Checksum:i41ABFF403E52F837
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC52746.1.
    RefSeqiNP_774121.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC52746; BAC52746; BAC52746.
    GeneIDi1054266.
    KEGGibja:blr7481.
    PATRICi21198941. VBIBraJap65052_7700.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC52746.1 .
    RefSeqi NP_774121.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali Q89DF8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.blr7481.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC52746 ; BAC52746 ; BAC52746 .
    GeneIDi 1054266.
    KEGGi bja:blr7481.
    PATRICi 21198941. VBIBraJap65052_7700.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OrthoDBi EOG644ZM1.
    PhylomeDBi Q89DF8.

    Enzyme and pathway databases

    BioCyci BJAP224911:GJEJ-7536-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiSYI_BRADU
    AccessioniPrimary (citable) accession number: Q89DF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3