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Q89DF8

- SYI_BRADU

UniProt

Q89DF8 - SYI_BRADU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301Aminoacyl-adenylateUniRule annotation
Binding sitei674 – 6741ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-7536-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:blr7481
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Isoleucine--tRNA ligasePRO_0000098360Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224911.blr7481.

Structurei

3D structure databases

ProteinModelPortaliQ89DF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 8011"HIGH" regionAdd
BLAST
Motifi671 – 6755"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
InParanoidiQ89DF8.
KOiK01870.
OrthoDBiEOG644ZM1.
PhylomeDBiQ89DF8.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89DF8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEKPQKSQK SEVKDYSKTL FLPQTEFPMR AGLPQREPEI LKYWNDIGLY
60 70 80 90 100
DRLRQEAEGR TKFVLHDGPP YANGNIHIGH ALNKILKDVV TKSQQMLGFD
110 120 130 140 150
SNYVPGWDCH GLPIEWKIEE ENYRKKGKQK PDFRDSAAMV AFRKECRAYA
160 170 180 190 200
THWINVQREE FKRLGIIGDW DHPYQTMSYP AEAQIARELM KFAANGTLYR
210 220 230 240 250
GSKPVMWSVV EKTALAEAEV EYEDYTSDMV WVKFPVTSPA HGALASASVV
260 270 280 290 300
IWTTTPWTLP GNRAISFSPK IAYGLYKVTD APADNWAKTG DLLILADALA
310 320 330 340 350
AEVFKQARVT TYEKVRELPA DTLDAVECAH PLKGFSGGYE FTVPLLPGDH
360 370 380 390 400
VTDDTGTGFV HTAPGHGRED FDVWMANARE LEARGINTTI PYTVDENGAF
410 420 430 440 450
TDHAPGFVGK RVINDKGEKG DANEAVIKAL IDAGKLLARG RLKHQYPHSW
460 470 480 490 500
RSKKPVIFRN TPQWFIAMDK DISANGHAKK GDTLRARALH AISVTQWVPP
510 520 530 540 550
SGENRINGMI ANRPDWVISR QRAWGVPIAV FVREKSDGSA EILQDEIVNQ
560 570 580 590 600
RIAEAFMEEG ADAWYMDGAR ERFLGSRASE DWKKVDDICD VWFDSGSTHA
610 620 630 640 650
FVLEDRQNFP QLGNIVRKVD GGSDTVMYLE GSDQHRGWFH SSLLESAGTR
660 670 680 690 700
GRAPYDIVLT HGFTLDENGR KMSKSLGNTV EPQKVIKDSG ADILRLWVCA
710 720 730 740 750
TDYADDQRIG PEILKNTIET YRKLRNSIRW MLGTLHHFKP SEKVAYAEMP
760 770 780 790 800
ELERLMLHEL AGHAETVRNA YAAFDYKTVV ASLAAFMNSE LSAFYFDIRK
810 820 830 840 850
DTLYCDPPSS PARKAALTTI DLLCDAILKW LAPILSFTTD EAWRMFRPNA
860 870 880 890 900
EPSVHLTLFP ADIEKLRDDK LAAKWETIRN VRRAVTGALE LERAAKNIGS
910 920 930 940 950
SLEASPVIYV ADRDMLATLF DTDLAEVCIT SNYEVRESEA PASAFRLDAV
960 970 980 990 1000
PGVAVVVEKA VGTKCARSWK ISQTVGEDPE YPDVTPRDAQ ALREWKALGV

GV
Length:1,002
Mass (Da):112,383
Last modified:June 1, 2003 - v1
Checksum:i41ABFF403E52F837
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC52746.1.
RefSeqiNP_774121.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC52746; BAC52746; BAC52746.
GeneIDi1054266.
KEGGibja:blr7481.
PATRICi21198941. VBIBraJap65052_7700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC52746.1 .
RefSeqi NP_774121.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89DF8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr7481.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC52746 ; BAC52746 ; BAC52746 .
GeneIDi 1054266.
KEGGi bja:blr7481.
PATRICi 21198941. VBIBraJap65052_7700.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
InParanoidi Q89DF8.
KOi K01870.
OrthoDBi EOG644ZM1.
PhylomeDBi Q89DF8.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-7536-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiSYI_BRADU
AccessioniPrimary (citable) accession number: Q89DF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3