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Q89DF8 (SYI_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:blr7481
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length1002 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10021002Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098360

Regions

Motif70 – 8011"HIGH" region HAMAP-Rule MF_02002
Motif671 – 6755"KMSKS" region HAMAP-Rule MF_02002

Sites

Binding site6301Aminoacyl-adenylate By similarity
Binding site6741ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89DF8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 41ABFF403E52F837

FASTA1,002112,383
        10         20         30         40         50         60 
MSEKPQKSQK SEVKDYSKTL FLPQTEFPMR AGLPQREPEI LKYWNDIGLY DRLRQEAEGR 

        70         80         90        100        110        120 
TKFVLHDGPP YANGNIHIGH ALNKILKDVV TKSQQMLGFD SNYVPGWDCH GLPIEWKIEE 

       130        140        150        160        170        180 
ENYRKKGKQK PDFRDSAAMV AFRKECRAYA THWINVQREE FKRLGIIGDW DHPYQTMSYP 

       190        200        210        220        230        240 
AEAQIARELM KFAANGTLYR GSKPVMWSVV EKTALAEAEV EYEDYTSDMV WVKFPVTSPA 

       250        260        270        280        290        300 
HGALASASVV IWTTTPWTLP GNRAISFSPK IAYGLYKVTD APADNWAKTG DLLILADALA 

       310        320        330        340        350        360 
AEVFKQARVT TYEKVRELPA DTLDAVECAH PLKGFSGGYE FTVPLLPGDH VTDDTGTGFV 

       370        380        390        400        410        420 
HTAPGHGRED FDVWMANARE LEARGINTTI PYTVDENGAF TDHAPGFVGK RVINDKGEKG 

       430        440        450        460        470        480 
DANEAVIKAL IDAGKLLARG RLKHQYPHSW RSKKPVIFRN TPQWFIAMDK DISANGHAKK 

       490        500        510        520        530        540 
GDTLRARALH AISVTQWVPP SGENRINGMI ANRPDWVISR QRAWGVPIAV FVREKSDGSA 

       550        560        570        580        590        600 
EILQDEIVNQ RIAEAFMEEG ADAWYMDGAR ERFLGSRASE DWKKVDDICD VWFDSGSTHA 

       610        620        630        640        650        660 
FVLEDRQNFP QLGNIVRKVD GGSDTVMYLE GSDQHRGWFH SSLLESAGTR GRAPYDIVLT 

       670        680        690        700        710        720 
HGFTLDENGR KMSKSLGNTV EPQKVIKDSG ADILRLWVCA TDYADDQRIG PEILKNTIET 

       730        740        750        760        770        780 
YRKLRNSIRW MLGTLHHFKP SEKVAYAEMP ELERLMLHEL AGHAETVRNA YAAFDYKTVV 

       790        800        810        820        830        840 
ASLAAFMNSE LSAFYFDIRK DTLYCDPPSS PARKAALTTI DLLCDAILKW LAPILSFTTD 

       850        860        870        880        890        900 
EAWRMFRPNA EPSVHLTLFP ADIEKLRDDK LAAKWETIRN VRRAVTGALE LERAAKNIGS 

       910        920        930        940        950        960 
SLEASPVIYV ADRDMLATLF DTDLAEVCIT SNYEVRESEA PASAFRLDAV PGVAVVVEKA 

       970        980        990       1000 
VGTKCARSWK ISQTVGEDPE YPDVTPRDAQ ALREWKALGV GV 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC52746.1.
RefSeqNP_774121.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89DF8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr7481.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC52746; BAC52746; BAC52746.
GeneID1054266.
KEGGbja:blr7481.
PATRIC21198941. VBIBraJap65052_7700.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OrthoDBEOG644ZM1.
PhylomeDBQ89DF8.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-7536-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_BRADU
AccessionPrimary (citable) accession number: Q89DF8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries