Q89BL4 (PYRE_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	blr8134

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.
Sequence caution	The sequence BAC53399.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110678
Regions
	Region	128 – 136	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	98	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	99	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	102	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	104	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	132	1	Orotate By similarity
	Binding site	160	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89BL4 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: 482ABB5AD697D4AF	FASTA	187	20,219
	10 20 30 40 50 60 MSKSASRARL FEIIRRRSFG RGEVTLASGR KSDFYFNLKP TMLDPEGATL LAELTYEALK 70 80 90 100 110 120 DDNLDFIGGL EMGAVPLAGA LAQISWIKGH PIAAFFVRKK PKEHGAKLAI EGLPRGETLQ 130 140 150 160 170 180 GKRVVIVEDV TTTGGSAMKA VESVRETGAE VVLVLTMVDR EEGATDTFGA AGLPFRSLYK ASEFLKA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC53399.1. Different initiation.
RefSeq	NP_774774.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89BL4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1048846.
GenomeReviews	Gene locus blr8134 in contig BA000040_GR.
KEGG	bja:blr8134.
NMPDR	fig|224911.1.peg.8134.
PATRIC	21200288. VBIBraJap65052_8370.
Phylogenomic databases
HOGENOM	HBG404341.
OMA	GKEADYY.
PhylomeDB	Q89BL4.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR8134-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BRAJA

Accession

Primary (citable) accession number: Q89BL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program