Elongation factor 4 - Bradyrhizobium japonicum (strain USDA 110)

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Q89BJ8 (LEPA_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 64. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Elongation factor 4
Short name=EF-4
EC=3.6.5.n1

Alternative name(s):
Ribosomal back-translocase LepA

Gene names

Name:	lepA
Ordered Locus Names:	blr8150

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium ›

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071
Catalytic activity	GTP + H₂O = GDP + phosphate. HAMAP-Rule MF_00071
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.
Sequence similarities	Belongs to the GTP-binding elongation factor family. LepA subfamily.
Sequence caution	The sequence BAC53415.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC positive regulation of translation Inferred from electronic annotation. Source: HAMAP translational elongation Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP ribosome binding Inferred from electronic annotation. Source: HAMAP translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 603

603

Elongation factor 4 HAMAP-Rule MF_00071

PRO_0000176243

Regions

Nucleotide binding

19 – 24

GTP By similarity

Nucleotide binding

138 – 141

GTP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q89BJ8 [UniParc].

Last modified July 19, 2003. Version 2.
Checksum: 75DB021BCB2102EF
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FASTA

603

66,784

        10         20         30         40         50         60 
MTTVPISNIR NFSIVAHIDH GKSTLADRLI QMTGGLTDRE MAGKEQVLDS MDIERERGIT 

        70         80         90        100        110        120 
IKAQTVRLAY RAKDGKDYIF NLMDTPGHVD FAYEVSRSLA ACEGSLLVVD ASQGVEAQTL 

       130        140        150        160        170        180 
ANVYQALDNN HEIVPVLNKV DLPAAEPEKV KQQIEDVIGI DASDAVMISA KTGLGVPDVL 

       190        200        210        220        230        240 
EAIVTRLPPP KGDRDATLKA LLVDSWYDVY LGVVVLIRVV DGVMKKGSRV RMMGTGAAYD 

       250        260        270        280        290        300 
VERVGFFTPK MTQVDELGPG EIGFITAAIK EVADTRVGDT ITDDRKPVTE MLPGFKPAIP 

       310        320        330        340        350        360 
VVFCGLFPVD ADDFETLRAA MGKLRLNDAS FSFEMETSAA LGFGFRCGFL GLLHLEIIQE 

       370        380        390        400        410        420 
RLSREFDLNL IATAPSVIYK MKLTDGTELE IHNPVDMPDV VKIEEIQEPW IEATILTPDE 

       430        440        450        460        470        480 
YLGSVLKLCQ DRRGAQKELT YVGARAMVKY DLPLNEVVFD FYDRLKSVSK GYASFDYHLT 

       490        500        510        520        530        540 
DYKPADLVKM QILVNAEPVD ALSMLVHRTR AEGRGRAMVE KMKELIPPHM FQIPIQAAIG 

       550        560        570        580        590        600 
GKVIARETVR ALRKDVTAKC YGGDITRKRK LLEKQKEGKK KMRQFGKVDI PQEAFIAALK 


VDS

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References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC53415.1. Different initiation.
RefSeq	NP_774790.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89BJ8.
ModBase	Search...
Protein-protein interaction databases
STRING	224911.blr8150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC53415; BAC53415; BAC53415.
GeneID	1048708.
KEGG	bja:blr8150.
PATRIC	21200320. VBIBraJap65052_8386.
Phylogenomic databases
eggNOG	COG0481.
HOGENOM	HOG000020624.
KO	K03596.
OMA	KCYGGDA.
ProtClustDB	PRK05433.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-8208-MONOMER.
Family and domain databases
Gene3D	3.30.70.240. 1 hit.
HAMAP	MF_00071. LepA.
InterPro	IPR006297. EF-4. IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR013842. LepA_GTP-bd_C. IPR005225. Small_GTP-bd_dom. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF40. PTHR23115:SF40. 1 hit.
Pfam	PF00679. EFG_C. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF06421. LepA_C. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR01393. lepA. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEPA_BRAJA

Accession

Primary (citable) accession number: Q89BJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	July 19, 2003
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents