Reviewed, UniProtKB/Swiss-Prot Q89B29 (RSMD_BUCBP)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA small subunit methyltransferase D
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase
    16S rRNA m2G966 methyltransferase

Gene names

Name:	rsmD
Ordered Locus Names:	bbp_025

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmD family.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methylation Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Ribosomal RNA small subunit methyltransferase D		PRO_0000216244

Sequences

	Sequence		Length	Mass (Da)
	Q89B29-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6967D74F4A27C737	FASTA	196	22,811
	10 20 30 40 50 60 MKILKKKSKI TSIQIISGKY KNSRIPIINT KNLRPTTNYI RETLFNWISN EKIKKSHCLD 70 80 90 100 110 120 CFSGSGALGI EAVSRYALSS TCIEIQKKAI FFLKKILIKL SITNVHVIRA NTIQWLKKPN 130 140 150 160 170 180 KSYDIIFLDP PFKTTLLKQT IALLIKNNWL KNNALIYIEQ ENTLNKLITP PNNWILKKEK 190 KTKKIHYSLY MNVNNF

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26768.1.
RefSeq	NP_777663.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1058366.
GenomeReviews	Gene locus bbp_025 in contig AE016826_GR.
KEGG	bab:bbp025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89B29.
OMA	Q89B29. LEMERGV.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_025-MON.
Family and domain databases
InterPro	IPR004398. CHP00095. IPR016065. DUF_methylase. IPR002052. N6_adenine_Mtase_CS. [Graphical view]
Pfam	PF03602. Cons_hypoth95. 1 hit. [Graphical view]
PIRSF	PIRSF004553. CHP00095. 1 hit.
TIGRFAMs	TIGR00095. CHP95. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMD_BUCBP

Accession

Primary (citable) accession number: Q89B29

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)