Q89B24 (METE_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	bbp_031

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098621
Sites
	Metal binding	643	1	Zinc By similarity
	Metal binding	645	1	Zinc By similarity
	Metal binding	728	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89B24 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: D99AF70DE7E2C81D	FASTA	756	87,674
	10 20 30 40 50 60 MINNHTLGFP RIGLFRELKV AQEKYWSRKI SKEELFSVGK TLRVRHWQQQ KELGINYIPV 70 80 90 100 110 120 GDFSWYDHVL GTSMLLGNIP KRHKNGNDLV TLDTLFRVAR GVAPTGASVS ASEMTKWFNT 130 140 150 160 170 180 NYHYIVPEFS IKSEFCFSWR QILDEIDEAL LLGHQVKPVI LGPLTYLWLG KVKGKKFDRL 190 200 210 220 230 240 NLLKEILPVY KYLLSEINDR NISWVQIDEP ILTLELPEKW KNAFRFAYQS LYGYNSLLLT 250 260 270 280 290 300 TYFGSVQHNF SLICELPIQG VHLDLVHGKY DLVFLNSFIP EKWLISLGII NGKNIWKADL 310 320 330 340 350 360 VMWFKRLQLF SKLRHSLWIG TSCSLLHVPI DLKLENKISS EVRSWFSFAL QKCQELTLLR 370 380 390 400 410 420 DALNNSNTVT EDIRVWSQPI HSRKKSAIVH NVDVKERLKS ITSNDFKRNN VFSVRKQKQH 430 440 450 460 470 480 KNLKLPILPT TTIGSFPQTA DIRKARFDFK KGNINHDQYN TFISKHIQNA ILKQEKLGID 490 500 510 520 530 540 VLVHGEFERN DMVEYFGEHL NGFVFTEFGW VQSYGSRCVK PPIIIGDVSR SKPISLDWIK 550 560 570 580 590 600 YAQTLTSKPV KGMLTGPVTI LLWSFVREDL SKKIISRQIA LALRDEVLDL EQSGVKIIQI 610 620 630 640 650 660 DEPALREGLP LRLSLRNEYL SWAVDSFKLS SSGVCDETQI HTHMCYCEFN DIMNAIVLLD 670 680 690 700 710 720 ADVITIETSR SDMELLEFFK EFKYPNDIGP GVYDIHSPNI PSIEWIMTLL RQAMHYIPVK 730 740 750 RLWVNPDCGL KTRTWDETYY SLENMVRAAK ILRKKL

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO26774.1.
RefSeq	NP_777669.1. NC_004545.1.
3D structure databases
ProteinModelPortal	Q89B24.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002646; EBBUCP00000002470; EBBUCG00000002646.
GeneID	1058349.
GenomeReviews	Gene locus bbp_031 in contig AE016826_GR.
KEGG	bab:bbp031.
PATRIC	21244833. VBIBucAph80364_0031.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007816.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q89B24.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_031-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BUCBP

Accession

Primary (citable) accession number: Q89B24

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program