Q89B23 (PUR9_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	purH
Ordered Locus Names:	bbp_032

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the PurH family.
Sequence caution	The sequence AAO26775.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Bifunctional purine biosynthesis protein PurH HAMAP MF_00139		PRO_0000192078

Sequences

	Sequence		Length	Mass (Da)
	Q89B23 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 940058B815B31047	FASTA	529	60,084
	10 20 30 40 50 60 MTNRNVIKNV LISVSDTSNI IEFSKSLISK NIKLFATKGT ANFLKKNNIY ATDITNYTNF 70 80 90 100 110 120 PEIMNGRIKT LHHKIYASIL AQPKHDKKTI EKYNIILMDI VVINFYPFEE ASNNTNLHLN 130 140 150 160 170 180 DIIEHIDIGG PAIVRAAAKN YKNVLVVTQP NLYQSIVNEM NLNNNIISET TKLKFATIAF 190 200 210 220 230 240 KHTMNYDNNI YQYLSKKNKT VPKNTQLQTL LPSHLTINFK KKQDLCYGEN KQQQASWYTN 250 260 270 280 290 300 TSKNTSGRMK IKQLQGKILS YNNLSDIHLA LSCIHEFNKT TCAIIKHGNP CGVATAKNND 310 320 330 340 350 360 QAYKLAYETD PISAFGGIIV FNQKLNDVTA RKIIKTQFSE IILAPDFTQE AKKIFDKKPN 370 380 390 400 410 420 LRIIKYDPNY NYLNYNIDIK SIYGDILVQS NTNSIININQ WDIVSKKRPN EQEINDAKFA 430 440 450 460 470 480 LRVVKHLKSN SIVLIKNQIT ISIGSGQTSR IDATKIAIYK ANNNNISLNH TTLASDAFFP 490 500 510 520 FSDSIDLISK SGITCIVQPG GSIRDNEIIM SANKYNISMI FTKQRYFKH

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO26775.1. Different initiation.
RefSeq	NP_777670.1. NC_004545.1.
3D structure databases
ProteinModelPortal	Q89B23.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002352; EBBUCP00000002176; EBBUCG00000002352.
GeneID	1058351.
GenomeReviews	Gene locus bbp_032 in contig AE016826_GR.
KEGG	bab:bbp032.
PATRIC	21244835. VBIBucAph80364_0032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008052.
HOGENOM	HBG498048.
OMA	FTGTRHF.
PhylomeDB	Q89B23.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_032-MONOMER.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR024051. AICAR_Tfase_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.140.20. G3DSA:3.40.140.20. 2 hits. G3DSA:3.40.50.1380. MGS-like_dom. 1 hit.
KO	K00602.
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. PurH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BUCBP

Accession

Primary (citable) accession number: Q89B23

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program