Reviewed, UniProtKB/Swiss-Prot Q89B23 (PUR9_BUCBP)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	bbp_032

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_0000192078

Sequences

	Sequence		Length	Mass (Da)
	Q89B23-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 940058B815B31047	FASTA	529	60,084
	10 20 30 40 50 60 MTNRNVIKNV LISVSDTSNI IEFSKSLISK NIKLFATKGT ANFLKKNNIY ATDITNYTNF 70 80 90 100 110 120 PEIMNGRIKT LHHKIYASIL AQPKHDKKTI EKYNIILMDI VVINFYPFEE ASNNTNLHLN 130 140 150 160 170 180 DIIEHIDIGG PAIVRAAAKN YKNVLVVTQP NLYQSIVNEM NLNNNIISET TKLKFATIAF 190 200 210 220 230 240 KHTMNYDNNI YQYLSKKNKT VPKNTQLQTL LPSHLTINFK KKQDLCYGEN KQQQASWYTN 250 260 270 280 290 300 TSKNTSGRMK IKQLQGKILS YNNLSDIHLA LSCIHEFNKT TCAIIKHGNP CGVATAKNND 310 320 330 340 350 360 QAYKLAYETD PISAFGGIIV FNQKLNDVTA RKIIKTQFSE IILAPDFTQE AKKIFDKKPN 370 380 390 400 410 420 LRIIKYDPNY NYLNYNIDIK SIYGDILVQS NTNSIININQ WDIVSKKRPN EQEINDAKFA 430 440 450 460 470 480 LRVVKHLKSN SIVLIKNQIT ISIGSGQTSR IDATKIAIYK ANNNNISLNH TTLASDAFFP 490 500 510 520 FSDSIDLISK SGITCIVQPG GSIRDNEIIM SANKYNISMI FTKQRYFKH

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO26775.1. Different initiation.
RefSeq	NP_777670.1.
3D structure databases
SMR	Q89B23. Positions 7-529.
ModBase	Search...
Genome annotation databases
GeneID	1058351.
GenomeReviews	Gene locus bbp_032 in contig AE016826_GR.
KEGG	bab:bbp032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG498048.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_032-MONOMER.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BUCBP

Accession

Primary (citable) accession number: Q89B23

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)