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Q89B13

- METF_BUCBP

UniProt

Q89B13 - METF_BUCBP

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei28 – 281Proton donor/acceptorBy similarity
    Binding sitei89 – 891FADBy similarity
    Binding sitei121 – 1211SubstrateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Binding sitei184 – 1841SubstrateBy similarity
    Binding sitei220 – 2201SubstrateBy similarity
    Binding sitei224 – 2241SubstrateBy similarity
    Binding sitei276 – 2761SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 336NADBy similarity
    Nucleotide bindingi59 – 624FADBy similarity
    Nucleotide bindingi59 – 602NADBy similarity
    Nucleotide bindingi119 – 1213FADBy similarity
    Nucleotide bindingi132 – 1332FADBy similarity
    Nucleotide bindingi157 – 1604FADBy similarity
    Nucleotide bindingi166 – 1738FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyciBAPH224915:GJ9D-47-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    Ordered Locus Names:bbp_047
    OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
    Taxonomic identifieri224915 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000601: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2952955,10-methylenetetrahydrofolate reductasePRO_0000190260Add
    BLAST

    Proteomic databases

    PRIDEiQ89B13.

    Interactioni

    Protein-protein interaction databases

    STRINGi224915.bbp047.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89B13.
    SMRiQ89B13. Positions 22-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    KOiK00297.
    OMAiFEFFPTK.
    OrthoDBiEOG6D2KVW.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q89B13-1 [UniParc]FASTAAdd to Basket

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    MKLVYKNYHE TLNQHLMNIC EKVNISFEFF PPNNILSEKN LWQVIDKLKL    50
    LTPKFFSVTH GTNSKIRATC TSNIVKKIKK YTGIETVPHL TCINSTEEEL 100
    KVIAKTYWDS GIRHILALRG DIFNTNCKPK IYAVDLIKLL KSIANFEISV 150
    AAYPEVHPEA VNAKYDIINL KRKVEAGATR AITQFFFNID CFLRFRDLCV 200
    KNNITIDIVP GIFPISNFKQ LLKFSSVSNV SIPKWLCCMF HGLDNDLNTS 250
    RIIGSSIAID MVKVLYSEGI RSFHFYTLNK SEISFAICKI LENKS 295
    Length:295
    Mass (Da):33,770
    Last modified:June 1, 2003 - v1
    Checksum:iD34F60F04D44AED3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016826 Genomic DNA. Translation: AAO26786.1.
    RefSeqiNP_777681.1. NC_004545.1.
    WP_011091187.1. NC_004545.1.

    Genome annotation databases

    EnsemblBacteriaiAAO26786; AAO26786; bbp_047.
    GeneIDi1058532.
    KEGGibab:bbp047.
    PATRICi21244867. VBIBucAph80364_0044.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016826 Genomic DNA. Translation: AAO26786.1 .
    RefSeqi NP_777681.1. NC_004545.1.
    WP_011091187.1. NC_004545.1.

    3D structure databases

    ProteinModelPortali Q89B13.
    SMRi Q89B13. Positions 22-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224915.bbp047.

    Proteomic databases

    PRIDEi Q89B13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO26786 ; AAO26786 ; bbp_047 .
    GeneIDi 1058532.
    KEGGi bab:bbp047.
    PATRICi 21244867. VBIBucAph80364_0044.

    Phylogenomic databases

    eggNOGi COG0685.
    KOi K00297.
    OMAi FEFFPTK.
    OrthoDBi EOG6D2KVW.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci BAPH224915:GJ9D-47-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bp.

    Entry informationi

    Entry nameiMETF_BUCBP
    AccessioniPrimary (citable) accession number: Q89B13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3