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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptorBy similarity
Binding sitei89 – 891FADBy similarity
Binding sitei121 – 1211SubstrateBy similarity
Binding sitei153 – 1531FADBy similarity
Binding sitei184 – 1841SubstrateBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei224 – 2241SubstrateBy similarity
Binding sitei276 – 2761SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NADBy similarity
Nucleotide bindingi59 – 624FADBy similarity
Nucleotide bindingi59 – 602NADBy similarity
Nucleotide bindingi119 – 1213FADBy similarity
Nucleotide bindingi132 – 1332FADBy similarity
Nucleotide bindingi157 – 1604FADBy similarity
Nucleotide bindingi166 – 1738FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-47-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:bbp_047
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000601 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2952955,10-methylenetetrahydrofolate reductasePRO_0000190260Add
BLAST

Proteomic databases

PRIDEiQ89B13.

Interactioni

Protein-protein interaction databases

STRINGi224915.bbp047.

Structurei

3D structure databases

ProteinModelPortaliQ89B13.
SMRiQ89B13. Positions 22-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
KOiK00297.
OMAiFIRAETG.
OrthoDBiEOG6D2KVW.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89B13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLVYKNYHE TLNQHLMNIC EKVNISFEFF PPNNILSEKN LWQVIDKLKL
60 70 80 90 100
LTPKFFSVTH GTNSKIRATC TSNIVKKIKK YTGIETVPHL TCINSTEEEL
110 120 130 140 150
KVIAKTYWDS GIRHILALRG DIFNTNCKPK IYAVDLIKLL KSIANFEISV
160 170 180 190 200
AAYPEVHPEA VNAKYDIINL KRKVEAGATR AITQFFFNID CFLRFRDLCV
210 220 230 240 250
KNNITIDIVP GIFPISNFKQ LLKFSSVSNV SIPKWLCCMF HGLDNDLNTS
260 270 280 290
RIIGSSIAID MVKVLYSEGI RSFHFYTLNK SEISFAICKI LENKS
Length:295
Mass (Da):33,770
Last modified:May 31, 2003 - v1
Checksum:iD34F60F04D44AED3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO26786.1.
RefSeqiNP_777681.1. NC_004545.1.
WP_011091187.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO26786; AAO26786; bbp_047.
GeneIDi1058532.
KEGGibab:bbp047.
PATRICi21244867. VBIBucAph80364_0044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO26786.1.
RefSeqiNP_777681.1. NC_004545.1.
WP_011091187.1. NC_004545.1.

3D structure databases

ProteinModelPortaliQ89B13.
SMRiQ89B13. Positions 22-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224915.bbp047.

Proteomic databases

PRIDEiQ89B13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO26786; AAO26786; bbp_047.
GeneIDi1058532.
KEGGibab:bbp047.
PATRICi21244867. VBIBucAph80364_0044.

Phylogenomic databases

eggNOGiCOG0685.
KOiK00297.
OMAiFIRAETG.
OrthoDBiEOG6D2KVW.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciBAPH224915:GJ9D-47-MONOMER.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiMETF_BUCBP
AccessioniPrimary (citable) accession number: Q89B13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 6, 2003
Last sequence update: May 31, 2003
Last modified: March 31, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.