Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei28Proton donor/acceptorBy similarity1
Binding sitei89FADBy similarity1
Binding sitei121SubstrateBy similarity1
Binding sitei153FADBy similarity1
Binding sitei184SubstrateBy similarity1
Binding sitei220SubstrateBy similarity1
Binding sitei224SubstrateBy similarity1
Binding sitei276SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 33NADBy similarity6
Nucleotide bindingi59 – 62FADBy similarity4
Nucleotide bindingi59 – 60NADBy similarity2
Nucleotide bindingi119 – 121FADBy similarity3
Nucleotide bindingi132 – 133FADBy similarity2
Nucleotide bindingi157 – 160FADBy similarity4
Nucleotide bindingi166 – 173FADBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:bbp_047
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000601 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902601 – 2955,10-methylenetetrahydrofolate reductaseAdd BLAST295

Proteomic databases

PRIDEiQ89B13.

Interactioni

Protein-protein interaction databases

STRINGi224915.bbp047.

Structurei

3D structure databases

ProteinModelPortaliQ89B13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105SYT. Bacteria.
COG0685. LUCA.
KOiK00297.
OMAiEMHPQAR.
OrthoDBiPOG091H02HI.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89B13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLVYKNYHE TLNQHLMNIC EKVNISFEFF PPNNILSEKN LWQVIDKLKL
60 70 80 90 100
LTPKFFSVTH GTNSKIRATC TSNIVKKIKK YTGIETVPHL TCINSTEEEL
110 120 130 140 150
KVIAKTYWDS GIRHILALRG DIFNTNCKPK IYAVDLIKLL KSIANFEISV
160 170 180 190 200
AAYPEVHPEA VNAKYDIINL KRKVEAGATR AITQFFFNID CFLRFRDLCV
210 220 230 240 250
KNNITIDIVP GIFPISNFKQ LLKFSSVSNV SIPKWLCCMF HGLDNDLNTS
260 270 280 290
RIIGSSIAID MVKVLYSEGI RSFHFYTLNK SEISFAICKI LENKS
Length:295
Mass (Da):33,770
Last modified:June 1, 2003 - v1
Checksum:iD34F60F04D44AED3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO26786.1.
RefSeqiWP_011091187.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO26786; AAO26786; bbp_047.
KEGGibab:bbp_047.
PATRICi21244867. VBIBucAph80364_0044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO26786.1.
RefSeqiWP_011091187.1. NC_004545.1.

3D structure databases

ProteinModelPortaliQ89B13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224915.bbp047.

Proteomic databases

PRIDEiQ89B13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO26786; AAO26786; bbp_047.
KEGGibab:bbp_047.
PATRICi21244867. VBIBucAph80364_0044.

Phylogenomic databases

eggNOGiENOG4105SYT. Bacteria.
COG0685. LUCA.
KOiK00297.
OMAiEMHPQAR.
OrthoDBiPOG091H02HI.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETF_BUCBP
AccessioniPrimary (citable) accession number: Q89B13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.