Q89B13 (METF_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydrofolate reductase
EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	bbp_047

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Baizongia pistaciae)

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	5,10-methylenetetrahydrofolate reductase		PRO_0000190260

Sequences

	Sequence		Length	Mass (Da)
	Q89B13 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D34F60F04D44AED3	FASTA	295	33,770
	10 20 30 40 50 60 MKLVYKNYHE TLNQHLMNIC EKVNISFEFF PPNNILSEKN LWQVIDKLKL LTPKFFSVTH 70 80 90 100 110 120 GTNSKIRATC TSNIVKKIKK YTGIETVPHL TCINSTEEEL KVIAKTYWDS GIRHILALRG 130 140 150 160 170 180 DIFNTNCKPK IYAVDLIKLL KSIANFEISV AAYPEVHPEA VNAKYDIINL KRKVEAGATR 190 200 210 220 230 240 AITQFFFNID CFLRFRDLCV KNNITIDIVP GIFPISNFKQ LLKFSSVSNV SIPKWLCCMF 250 260 270 280 290 HGLDNDLNTS RIIGSSIAID MVKVLYSEGI RSFHFYTLNK SEISFAICKI LENKS

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO26786.1.
RefSeq	NP_777681.1. NC_004545.1.
3D structure databases
ProteinModelPortal	Q89B13.
SMR	Q89B13. Positions 22-291.
ModBase	Search...
Protein-protein interaction databases
STRING	224915.bbp047.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO26786; AAO26786; bbp_047.
GeneID	1058532.
KEGG	bab:bbp047.
PATRIC	21244867. VBIBucAph80364_0044.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0685.
KO	K00297.
OMA	SITQMFF.
ProtClustDB	PRK09432.
Enzyme and pathway databases
BioCyc	BAPH224915:GJ9D-47-MONOMER.
UniPathway	UPA00193.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_BUCBP

Accession

Primary (citable) accession number: Q89B13

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program