Reviewed, UniProtKB/Swiss-Prot Q89B13 (METF_BUCBP)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	bbp_047

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	5,10-methylenetetrahydrofolate reductase		PRO_0000190260

Sequences

	Sequence		Length	Mass (Da)
	Q89B13-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D34F60F04D44AED3	FASTA	295	33,770
	10 20 30 40 50 60 MKLVYKNYHE TLNQHLMNIC EKVNISFEFF PPNNILSEKN LWQVIDKLKL LTPKFFSVTH 70 80 90 100 110 120 GTNSKIRATC TSNIVKKIKK YTGIETVPHL TCINSTEEEL KVIAKTYWDS GIRHILALRG 130 140 150 160 170 180 DIFNTNCKPK IYAVDLIKLL KSIANFEISV AAYPEVHPEA VNAKYDIINL KRKVEAGATR 190 200 210 220 230 240 AITQFFFNID CFLRFRDLCV KNNITIDIVP GIFPISNFKQ LLKFSSVSNV SIPKWLCCMF 250 260 270 280 290 HGLDNDLNTS RIIGSSIAID MVKVLYSEGI RSFHFYTLNK SEISFAICKI LENKS

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26786.1.
RefSeq	NP_777681.1.
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Genome annotation databases
GeneID	1058532.
GenomeReviews	Gene locus bbp_047 in contig AE016826_GR.
KEGG	bab:bbp047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89B13.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_047-MON.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_BUCBP

Accession

Primary (citable) accession number: Q89B13

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)