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Reviewed, UniProtKB/Swiss-Prot Q89B06 (CCA_BUCBP)

Last modified May 5, 2009. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    CCA-adding enzyme
    EC=2.7.7.25
    EC=2.7.7.21
Alternative name(s):
    tRNA nucleotidyltransferase
    tRNA adenylyl-/cytidylyl- transferase
    tRNA CCA-pyrophosphorylase
    tRNA-NT
Gene names
Name: cca
Ordered Locus Names: bbp_057
OrganismBuchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]
Taxonomic identifier135842 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity.

Catalytic activity

ATP + tRNA(n) = diphosphate + tRNA(n+1). HAMAP MF_01262

CTP + tRNA(n) = diphosphate + tRNA(n+1). HAMAP MF_01262

Cofactor

Magnesium By similarity.

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity.

Sequence similarities

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419CCA-adding enzyme HAMAP MF_01262
PRO_0000139019

Sites

Metal binding211Magnesium By similarity
Metal binding231Magnesium By similarity
Binding site81ATP or CTP; via amide nitrogen By similarity
Binding site111ATP or CTP By similarity
Binding site911ATP or CTP By similarity
Binding site1371ATP or CTP By similarity
Binding site1401ATP or CTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89B06-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: A8C5D8926575D90A

FASTA41949,614
        10         20         30         40         50         60 
MKVYLVGGAI RNKFLNLPVQ DRDWVVVGAT PEILLSLKFK QVGKGFPVFL HPYSKEEYSL 

        70         80         90        100        110        120 
ARVDRKIGVG HTGFSFDYSN KVTLKEDLMR RDLTINAIAQ DNNGNYIDPF KGIRDIKNRI 

       130        140        150        160        170        180 
LRHVSPAFSE DPLRVLRIAR FCALFHHLGF RIATETMKIM SIVVKNNELL NLTRDRVWKE 

       190        200        210        220        230        240 
TEKAFNTDNP HVYFQVLKNC NALSVIFPEI NLVYQRQYYC IDNMYHNFYD TFDIFMGLAE 

       250        260        270        280        290        300 
LSKISRDIDI RFSYLFFCIN RMLFIDTSSY ILVINQKELV RYFKALCQRF CIPAYIKNVS 

       310        320        330        340        350        360 
ICFSRFYKFL SVIHYQSSKD IIMFFYIIDA WRKPYMIRKL SVLNNFCVSR NAYFKNITCQ 

       370        380        390        400        410 
QYPRNFLKYA FNVANKISIK PILKMGFSGL QIKYELIRLR INAIENWRQN ITVYKKCCF 

« Hide

Cross-references

Sequence databases

AE016826 Genomic DNA. Translation: AAO26796.1.
RefSeqNP_777691.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1058541.
GenomeReviewsGene locus bbp_057 in contig AE016826_GR.
KEGGbab:bbp057.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ89B06.
OMAQ89B06. NPHVYFQ.

Enzyme and pathway databases

BioCycBAPH224915:BBP_057-MON.

Family and domain databases

HAMAPMF_01262.
[Tree]
InterProIPR012006. CCA_bact.
IPR002646. PolyA_pol_reg.
[Graphical view]
PfamPF01743. PolyA_pol. 1 hit.
[Graphical view]
PIRSFPIRSF000813. CCA_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCCA_BUCBP
AccessionPrimary (citable) accession number: Q89B06
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: May 5, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents