Q89B06 (CCA_BUCBP) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 56.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: CCA-adding enzyme EC=2.7.7.72 Alternative name(s): CCA tRNA nucleotidyltransferase tRNA CCA-pyrophosphorylase tRNA adenylyl-/cytidylyl- transferase tRNA nucleotidyltransferase tRNA-NT | ||||
| Gene names |
| ||||
| Organism | Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 224915 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera |
Protein attributes
| Sequence length | 419 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity. HAMAP MF_01262 |
| Catalytic activity | A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate. HAMAP MF_01262 |
| Cofactor | Magnesium By similarity. HAMAP MF_01262 |
| Miscellaneous | A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity. HAMAP MF_01262 |
| Sequence similarities | Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | RNA repair tRNA processing |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding |
| Molecular function | Nucleotidyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | RNA repair Inferred from electronic annotation. Source: UniProtKB-KW tRNA 3'-terminal CCA additionInferred from electronic annotation. Source: InterPro |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA bindingInferred from electronic annotation. Source: UniProtKB-KW metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW tRNA adenylyltransferase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 419 | 419 | CCA-adding enzyme HAMAP MF_01262 | PRO_0000139019 | |||||
Sites | |||||||||
| Metal binding | 21 | 1 | Magnesium By similarity | ||||||
| Metal binding | 23 | 1 | Magnesium By similarity | ||||||
| Binding site | 8 | 1 | ATP or CTP; via amide nitrogen By similarity | ||||||
| Binding site | 11 | 1 | ATP or CTP By similarity | ||||||
| Binding site | 91 | 1 | ATP or CTP By similarity | ||||||
| Binding site | 137 | 1 | ATP or CTP By similarity | ||||||
| Binding site | 140 | 1 | ATP or CTP By similarity | ||||||
Sequences
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References
| [1] | "Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE016826 Genomic DNA. Translation: AAO26796.1. |
| RefSeq | NP_777691.1. NC_004545.1. |
3D structure databases | |
| ProteinModelPortal | Q89B06. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBUCT00000002518; EBBUCP00000002342; EBBUCG00000002518. |
| GeneID | 1058541. |
| GenomeReviews | Gene locus bbp_057 in contig AE016826_GR. |
| KEGG | bab:bbp057. |
| PATRIC | 21244887. VBIBucAph80364_0054. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| GeneTree | EBGT00050000008009. |
| HOGENOM | HBG635063. |
| OMA | QVGKGFP. |
| ProtClustDB | PRK13298. |
Enzyme and pathway databases | |
| BioCyc | BAPH224915:BBP_057-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01262. CCA_bact_type2. [Tree] |
| InterPro | IPR012006. CCA_bact. IPR002646. PolA_pol_head_dom. [Graphical view] |
| KO | K00974. |
| Pfam | PF01743. PolyA_pol. 1 hit. [Graphical view] |
| PIRSF | PIRSF000813. CCA_bact. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | CCA_BUCBP | ||||||||
| Accession | Primary (citable) accession number: Q89B06 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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