Reviewed, UniProtKB/Swiss-Prot Q89AX4 (SYM_BUCBP)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	bbp_103

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00098
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139113
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	329 – 333	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	332	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AX4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9AB9D07B367F6576	FASTA	545	63,701
	10 20 30 40 50 60 MKKRILVTCA FPYANGSLHI GHFLEHIQAD IWVRYKKMRG HEVWFICADD AHGTPIMLKS 70 80 90 100 110 120 QSLKMSPESF ISDIYKDHVN DLEKFNINYD NYYSTHSSEN SYFLKKIYHI LDKKGLIQTR 130 140 150 160 170 180 NIFQLFDNTK RVFLPDRFVK GACPICHTKD QYGDHCEVCG SSYSAVELIK PVSMLSGNCP 190 200 210 220 230 240 ILKKSLHFFF NLPYFESMLR SWVMSGVLQA SVVKKLDEWF KLGLREWDIS RDSPYFGFNI 250 260 270 280 290 300 PGFLDKYFYV WLDAPIGYIS TFKNLCTQRN NLNFLDFWKK NSECELYQFI GKDIVYFHSL 310 320 330 340 350 360 FWPSILEASN FRKPTKIFVH GHVTINGLKL SKSRGDCILA KNWIKNLDSD SLRYYYASKL 370 380 390 400 410 420 SSKIQDIEVN AKHFLYKINS DVVNKIVNLA SRVSSFINIY FNNELSSRID DLALYKRFVT 430 440 450 460 470 480 SSSYIEKMLE NCEFNSAISM VISLADIANS YVDNKKPWNL TKNIKNSNTL HDICTTVLNL 490 500 510 520 530 540 FRILMTWLKP VMPDLAKNSE KFLNIKLEWA NICIPLLNHK ISIFKALRHR IEDKQIKFLL PNNFE

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26838.1.
RefSeq	NP_777733.1.
3D structure databases
HSSP	HSSP built from PDB template 1MEA based on UniProtKB P00959.
ModBase	Search...
Genome annotation databases
GeneID	1058207.
GenomeReviews	Gene locus bbp_103 in contig AE016826_GR.
KEGG	bab:bbp103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AX4.
OMA	DFSWREF.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_103-MON.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BUCBP

Accession

Primary (citable) accession number: Q89AX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)