Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q89AV2 (DYR_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:folA
Ordered Locus Names:bbp_133
OrganismBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]
Taxonomic identifier224915 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length164 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 164164Dihydrofolate reductase
PRO_0000186385

Regions

Domain2 – 162161DHFR
Nucleotide binding7 – 82NADP By similarity
Nucleotide binding15 – 206NADP By similarity
Nucleotide binding44 – 474NADP By similarity
Nucleotide binding63 – 664NADP By similarity
Nucleotide binding96 – 1016NADP By similarity
Region6 – 83Substrate binding By similarity

Sites

Binding site281Substrate By similarity
Binding site581Substrate By similarity
Binding site1151Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89AV2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: DCCF58465EED5949

FASTA16419,189
        10         20         30         40         50         60 
MNISIIVAMS QNLVIGQKNS IPWNIPKDLS WFKKHTIKKS IIMGRKTWES IGRVLPMRQN 

        70         80         90        100        110        120 
IVLTRQKNIK NTNVLFVNSI SKAIQSALYK NEIMIIGGSN LYNQMLTSAN KLYITHIEKY 

       130        140        150        160 
ILGDTYFPTY DHLPWKIIFK KKIIEHEKTK NSFYVTFKIL KKIT 

« Hide

References

[1]"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016826 Genomic DNA. Translation: AAO26867.1.
RefSeqNP_777762.1. NC_004545.1.

3D structure databases

ProteinModelPortalQ89AV2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224915.bbp133.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO26867; AAO26867; bbp_133.
GeneID1058169.
KEGGbab:bbp133.
PATRIC21245045. VBIBucAph80364_0129.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0262.
KOK00287.
OMADTQFPDW.
OrthoDBEOG6KT2V2.
ProtClustDBCLSK315509.

Enzyme and pathway databases

BioCycBAPH224915:GJ9D-133-MONOMER.
UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_BUCBP
AccessionPrimary (citable) accession number: Q89AV2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: November 13, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways