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Q89AU9

- SYI_BUCBP

UniProt

Q89AU9 - SYI_BUCBP

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei562 – 5621Aminoacyl-adenylateUniRule annotation
Binding sitei606 – 6061ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-139-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:bbp_139
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000601: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligasePRO_0000098366Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224915.bbp139.

Structurei

3D structure databases

ProteinModelPortaliQ89AU9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi603 – 6075"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89AU9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDYKTTLNL PKTNFSMKGN LSKKEPFILK KWNDNNIYSL IKKQNIGKKK
60 70 80 90 100
FFLNDGPPYA NGNIHIGHAI NKILKDIVIK FKTLSGFDTY YTPSWDCHGL
110 120 130 140 150
PIEHKVEKTI KKENILITKK EFRIKCRNYA QSQVNNQKLE FIRLGVFGDW
160 170 180 190 200
ENSYLTMNFK NEANIARTLM KMFKYGYVYQ DFKPVHWCIN CKSALAEAEV
210 220 230 240 250
EYHNKLSNSI IIKFKLAHNI NFWNQIFKND HNQDIHLVVW TTTPWTLPAS
260 270 280 290 300
QAIALNPNFK YQLIQTNNNL FIISEKSVPD TMNKMGIQKW KKIHIFLGKN
310 320 330 340 350
ISNLKVIHPF LNTTIPVILA DHVTQELGTG IVHTAPEFGQ DDYYACKKNN
360 370 380 390 400
INFTPTIDKK GHFLNNIHPK LNNINIFKSI KIVIKLLNNN NALLHSEKLV
410 420 430 440 450
HSYPYCWRHK TPIISRATQQ WFININHNNL RNRCIKYIQE VKWIPHWSKN
460 470 480 490 500
RMIEMIINRP DWCISRQRTW GVPIPIFIHR KTGKLHPDTI KLSQKIIQNI
510 520 530 540 550
ETNGIQAWFD ITEKSFLGEL FKQYKKVTDV IDVWFESGSI QLSNIYNKIQ
560 570 580 590 600
HQHNNISDLY IEGLDQHRGW FMSSLIISAA ISNQTPYKKV ITHGFVVDKN
610 620 630 640 650
KKKMSKSIGN TVHPSEVINT LGSDILRLWT ASTNYSKEMS ISQETLIHIS
660 670 680 690 700
DYYRRIRNTA RFLLANLHEF NPENDLINAK NMIILDKWAI GKALHIQKKI
710 720 730 740 750
IKSYKNYNFH DVIKYLMNFC SLDMGTFYLE IIKDRQYTTQ KNSIARRSCQ
760 770 780 790 800
TAMYLILTAF VKWITPILPF TSDELWEYIP GTNKNKFVFL ELWSNQLFDL
810 820 830 840 850
NHKDTMNHEY WNQLLIIKTE VNKALEHARQ NKIIRKSLEA HMTLYVNETI
860 870 880 890 900
KCNLKLLNKE LKFLFITSKV EIKNFYEAPK DAFQSETITN FKTIIKKMNG
910 920 930
IKCPRCWHII TKIKNNKNHE ICKRCILNTI GPGELRQFL
Length:939
Mass (Da):110,381
Last modified:June 1, 2003 - v1
Checksum:iAF3D7233544B22EC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26873.1.
RefSeqiNP_777768.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO26873; AAO26873; bbp_139.
GeneIDi1058147.
KEGGibab:bbp139.
PATRICi21245057. VBIBucAph80364_0135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26873.1 .
RefSeqi NP_777768.1. NC_004545.1.

3D structure databases

ProteinModelPortali Q89AU9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224915.bbp139.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO26873 ; AAO26873 ; bbp_139 .
GeneIDi 1058147.
KEGGi bab:bbp139.
PATRICi 21245057. VBIBucAph80364_0135.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BAPH224915:GJ9D-139-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiSYI_BUCBP
AccessioniPrimary (citable) accession number: Q89AU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3