Q89AT2 (FOLC_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein folC

Including the following 2 domains:

1. Folylpolyglutamate synthase
   EC=6.3.2.17
   Alternative name(s):
   Folylpoly-gamma-glutamate synthetase
   Short name=FPGS
   Tetrahydrofolylpolyglutamate synthase
   Short name=Tetrahydrofolate synthase
2. Dihydrofolate synthase
   EC=6.3.2.12

Gene names

Name:	folC
Ordered Locus Names:	bbp_157

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of folates to polyglutamate derivatives.
Catalytic activity	ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1). ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.
Sequence similarities	Belongs to the folylpolyglutamate synthase family.

Ontologies

Keywords
Biological process	Folate biosynthesis One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dihydrofolate synthase activity Inferred from electronic annotation. Source: EC tetrahydrofolylpolyglutamate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Bifunctional protein folC		PRO_0000168302
Regions
	Nucleotide binding	55 – 61	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AT2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0D4446A212458EB7	FASTA	426	48,571
	10 20 30 40 50 60 MINKKLARSF SLYEWLYYLD HFMLDNIDPT LNRVFYVAKK LGVLKSKAFV FIVGGTNGKG 70 80 90 100 110 120 STCHVLENLL LNSGYRVGLY TSPHLMRYTE RVRINGFELE HLYHISAFND VKYFQNDVLL 130 140 150 160 170 180 TRFEFITLSA LILFKSYNLD IIILEVGLGG RLDATNILSA DVSVITNIDI DHSKILGVNR 190 200 210 220 230 240 SSISVEKSGI FRKNKIAIVA DNNFPKVAQY LAKKKKVRLR IVNIDWIYKK IEFEWSFCSS 250 260 270 280 290 300 KITWLHLPLP RNVSLDSVAT ALSAVSESGI KINQKVFRSC ISEITLCGRF ETISYNPIII 310 320 330 340 350 360 LDVAHNPHSA RYLFKKMSSF KKNGNIFAVV GILKEKNIKD IVSPLIPIVD YWYCITLLTH 370 380 390 400 410 420 RSATSSEIIK YLPNHNSQIS KNMTVALEKI FDKVTNNDIV LIFGSFITVC EANKFLANKV KNFKLL

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO26891.1.
RefSeq	NP_777786.1. NC_004545.1.
3D structure databases
ProteinModelPortal	Q89AT2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002261; EBBUCP00000002085; EBBUCG00000002261.
GeneID	1058567.
GenomeReviews	Gene locus bbp_157 in contig AE016826_GR.
KEGG	bab:bbp157.
PATRIC	21245093. VBIBucAph80364_0153.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008195.
HOGENOM	HBG744947.
OMA	ICEETHE.
ProtClustDB	CLSK315624.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_157-MONOMER.
Family and domain databases
InterPro	IPR001645. Folylpolyglutamate_synth. IPR018109. Folylpolyglutamate_synth_CS. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KO	K11754.
PANTHER	PTHR11136. Fpolygl_synthtse. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
PIRSF	PIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01499. FolC. 1 hit.
PROSITE	PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit. PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLC_BUCBP

Accession

Primary (citable) accession number: Q89AT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program