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Q89AS4

- RIR1_BUCBP

UniProt

Q89AS4 - RIR1_BUCBP

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Protein
Ribonucleoside-diphosphate reductase subunit alpha
Gene
nrdA, bbp_168
Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the alpha subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91Allosteric activator By similarity
Binding sitei55 – 551Allosteric activator By similarity
Binding sitei91 – 911Allosteric activator By similarity
Binding sitei209 – 2091Substrate By similarity
Sitei225 – 2251Important for hydrogen atom transfer By similarity
Sitei232 – 2321Allosteric effector binding By similarity
Binding sitei253 – 2531Substrate; via amide nitrogen By similarity
Sitei262 – 2621Allosteric effector binding By similarity
Active sitei437 – 4371Proton acceptor By similarity
Active sitei439 – 4391Cysteine radical intermediate By similarity
Active sitei441 – 4411Proton acceptor By similarity
Sitei462 – 4621Important for hydrogen atom transfer By similarity
Sitei730 – 7301Important for electron transfer By similarity
Sitei731 – 7311Important for electron transfer By similarity
Sitei754 – 7541Interacts with thioredoxin/glutaredoxin By similarity
Sitei759 – 7591Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-168-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:bbp_168
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000601: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 761761Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187208Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi225 ↔ 462Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi224915.bbp168.

Structurei

3D structure databases

ProteinModelPortaliQ89AS4.
SMRiQ89AS4. Positions 5-737.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 9591ATP-cone
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 217Allosteric activator binding By similarity
Regioni224 – 2252Substrate binding By similarity
Regioni437 – 4415Substrate binding By similarity
Regioni621 – 6255Substrate binding By similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.

Phylogenomic databases

eggNOGiCOG0209.
KOiK00525.
OMAiLLWQMPS.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89AS4-1 [UniParc]FASTAAdd to Basket

« Hide

MNQMLFVTKR NGKIELINLD KIHRVLNWAA KGLENISISQ VALKSRIQFY    50
NNIKTTIIHE TIIKAAADLI SENSPDYQYM AARLTIFHLR KKAFGQFNPP 100
NLYDHVKNMI KLEKYDQHLL NDYPKQDFLI MNSFIKHRRD MNFSYAAVKQ 150
LEGKYLIQNR VTKKIYESAQ FLYILISACL FSKYPNETRM KYIKNFYDAI 200
STFKISLPTP IMAGLRTPTR QFSSCVLIES EDNLNSINAT TSAIVKYVSQ 250
RAGIGINAGR IRALGSPIRN GDTLHTGCIP FYKHFQSAVK SCSQGGVRGG 300
AATIFYPIWH FEIESLLVLK NNRGIEENRV RHMDYSVQLN KLMYQRLILG 350
EHITLFSPSD VPNLYDSFFN NQEKFERLYI KYENDKTIRK KKVKASYLFS 400
LMMQERTSTG RIYIQNVDHC NSHSAFNPKI APIRQSNLCL EITLPTKALQ 450
DVHDKNGEIS LCTLSAINLG SLNNLNDLSK LSNLIVRALD SVLDYQEYPI 500
LSAKKSALSR RTLGIGVINF AYYLAKNGVR YSDGSANNLT HRTFEAIQYH 550
LLNASCVLAQ EKGACHWFSH TNYYKGILPI DTYKKNVDEI CNEPLHLDWE 600
LLRKKIKKYG LRNSTLSALM PSETSSQISN ATNGIEPPRG LISIKASKDG 650
MLRQVVPEYK RLKNNYELLW NIPNNTGYLQ LASIMQKFVD QSISANTNYD 700
PKLFPNNKIP MQQLILDLLT AYKLGLKTLY YQNTRDGASD HQTEDIQFSK 750
NENCNHGACK I 761
Length:761
Mass (Da):87,124
Last modified:June 1, 2003 - v1
Checksum:i7FB14E136283BDCF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26901.1.
RefSeqiNP_777796.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO26901; AAO26901; bbp_168.
GeneIDi1058219.
KEGGibab:bbp168.
PATRICi21245113. VBIBucAph80364_0163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26901.1 .
RefSeqi NP_777796.1. NC_004545.1.

3D structure databases

ProteinModelPortali Q89AS4.
SMRi Q89AS4. Positions 5-737.
ModBasei Search...

Protein-protein interaction databases

STRINGi 224915.bbp168.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO26901 ; AAO26901 ; bbp_168 .
GeneIDi 1058219.
KEGGi bab:bbp168.
PATRICi 21245113. VBIBucAph80364_0163.

Phylogenomic databases

eggNOGi COG0209.
KOi K00525.
OMAi LLWQMPS.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci BAPH224915:GJ9D-168-MONOMER.

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiRIR1_BUCBP
AccessioniPrimary (citable) accession number: Q89AS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi