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Q89AS4

- RIR1_BUCBP

UniProt

Q89AS4 - RIR1_BUCBP

Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Enzyme regulationi

    Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the alpha subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91Allosteric activatorBy similarity
    Binding sitei55 – 551Allosteric activatorBy similarity
    Binding sitei91 – 911Allosteric activatorBy similarity
    Binding sitei209 – 2091SubstrateBy similarity
    Sitei225 – 2251Important for hydrogen atom transferBy similarity
    Sitei232 – 2321Allosteric effector bindingBy similarity
    Binding sitei253 – 2531Substrate; via amide nitrogenBy similarity
    Sitei262 – 2621Allosteric effector bindingBy similarity
    Active sitei437 – 4371Proton acceptorBy similarity
    Active sitei439 – 4391Cysteine radical intermediateBy similarity
    Active sitei441 – 4411Proton acceptorBy similarity
    Sitei462 – 4621Important for hydrogen atom transferBy similarity
    Sitei730 – 7301Important for electron transferBy similarity
    Sitei731 – 7311Important for electron transferBy similarity
    Sitei754 – 7541Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei759 – 7591Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH224915:GJ9D-168-MONOMER.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase
    Gene namesi
    Name:nrdA
    Ordered Locus Names:bbp_168
    OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
    Taxonomic identifieri224915 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000601: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 761761Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187208Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi225 ↔ 462Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi224915.bbp168.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89AS4.
    SMRiQ89AS4. Positions 5-737.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 9591ATP-conePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni15 – 217Allosteric activator bindingBy similarity
    Regioni224 – 2252Substrate bindingBy similarity
    Regioni437 – 4415Substrate bindingBy similarity
    Regioni621 – 6255Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 ATP-cone domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0209.
    KOiK00525.
    OMAiLLWQMPS.
    OrthoDBiEOG6J48HC.

    Family and domain databases

    InterProiIPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view]
    PfamiPF03477. ATP-cone. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF48168. SSF48168. 1 hit.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS51161. ATP_CONE. 1 hit.
    PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q89AS4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNQMLFVTKR NGKIELINLD KIHRVLNWAA KGLENISISQ VALKSRIQFY    50
    NNIKTTIIHE TIIKAAADLI SENSPDYQYM AARLTIFHLR KKAFGQFNPP 100
    NLYDHVKNMI KLEKYDQHLL NDYPKQDFLI MNSFIKHRRD MNFSYAAVKQ 150
    LEGKYLIQNR VTKKIYESAQ FLYILISACL FSKYPNETRM KYIKNFYDAI 200
    STFKISLPTP IMAGLRTPTR QFSSCVLIES EDNLNSINAT TSAIVKYVSQ 250
    RAGIGINAGR IRALGSPIRN GDTLHTGCIP FYKHFQSAVK SCSQGGVRGG 300
    AATIFYPIWH FEIESLLVLK NNRGIEENRV RHMDYSVQLN KLMYQRLILG 350
    EHITLFSPSD VPNLYDSFFN NQEKFERLYI KYENDKTIRK KKVKASYLFS 400
    LMMQERTSTG RIYIQNVDHC NSHSAFNPKI APIRQSNLCL EITLPTKALQ 450
    DVHDKNGEIS LCTLSAINLG SLNNLNDLSK LSNLIVRALD SVLDYQEYPI 500
    LSAKKSALSR RTLGIGVINF AYYLAKNGVR YSDGSANNLT HRTFEAIQYH 550
    LLNASCVLAQ EKGACHWFSH TNYYKGILPI DTYKKNVDEI CNEPLHLDWE 600
    LLRKKIKKYG LRNSTLSALM PSETSSQISN ATNGIEPPRG LISIKASKDG 650
    MLRQVVPEYK RLKNNYELLW NIPNNTGYLQ LASIMQKFVD QSISANTNYD 700
    PKLFPNNKIP MQQLILDLLT AYKLGLKTLY YQNTRDGASD HQTEDIQFSK 750
    NENCNHGACK I 761
    Length:761
    Mass (Da):87,124
    Last modified:June 1, 2003 - v1
    Checksum:i7FB14E136283BDCF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016826 Genomic DNA. Translation: AAO26901.1.
    RefSeqiNP_777796.1. NC_004545.1.

    Genome annotation databases

    EnsemblBacteriaiAAO26901; AAO26901; bbp_168.
    GeneIDi1058219.
    KEGGibab:bbp168.
    PATRICi21245113. VBIBucAph80364_0163.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016826 Genomic DNA. Translation: AAO26901.1 .
    RefSeqi NP_777796.1. NC_004545.1.

    3D structure databases

    ProteinModelPortali Q89AS4.
    SMRi Q89AS4. Positions 5-737.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224915.bbp168.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO26901 ; AAO26901 ; bbp_168 .
    GeneIDi 1058219.
    KEGGi bab:bbp168.
    PATRICi 21245113. VBIBucAph80364_0163.

    Phylogenomic databases

    eggNOGi COG0209.
    KOi K00525.
    OMAi LLWQMPS.
    OrthoDBi EOG6J48HC.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    BioCyci BAPH224915:GJ9D-168-MONOMER.

    Family and domain databases

    InterProi IPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view ]
    Pfami PF03477. ATP-cone. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF48168. SSF48168. 1 hit.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS51161. ATP_CONE. 1 hit.
    PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bp.

    Entry informationi

    Entry nameiRIR1_BUCBP
    AccessioniPrimary (citable) accession number: Q89AS4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3