Reviewed, UniProtKB/Swiss-Prot Q89AS3 (GYRA_BUCBP)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	bbp_169

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	847 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 847	847	DNA gyrase subunit A		PRO_0000145225
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AS3-1 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: BCF78EEB59DD9939	FASTA	847	95,830
	10 20 30 40 50 60 MKEVAKEIIK IDIEDELKNS YLDYAMSVII GRALPDVRDG LKPVHRRILF AMKVLNNDWN 70 80 90 100 110 120 KTYKKSARIV GDVIGKYHPH GDTAVYDAIV RMAQPFSLRY VLIDGQGNFG SIDGDSAAAM 130 140 150 160 170 180 RYTEIRMSKI AYELLNDLDK NTVSFFSNYD GTEKIPEVLP AKIPNLLING SSGIAVGMAT 190 200 210 220 230 240 NIPPHNIKEV INGCLAFIDD QNITLKKLME HIPGPDFPTA GLINGKRGIE KAYKTGKGKI 250 260 270 280 290 300 YIRAKSIIEI QKKTKKKSII IYELPYQVNK ARVIKGIANL VKEKKIEGIT TLRDESDKEG 310 320 330 340 350 360 MRIVIEIKKE TKAEIILNQL YSLTQLEISF GINMVALIHG QPKVMTLKEI LNAFINHRRK 370 380 390 400 410 420 IIMRRSLFEL NKIRKKIHIL EGLIISLDNI DLIINLIKKS STLEEAKNKL KTYHWYSKHA 430 440 450 460 470 480 QYTQILKKNA HSLTPYDKKL RPIDTQKFFL TPEQIQAILE LRLQKLTHLE HKKLISEYKQ 490 500 510 520 530 540 LFKTSNNLEN ILKNNNILTK IMKDELIKIR DNFGDKRRTK INVNYSDINT SDLINKENVV 550 560 570 580 590 600 ITLSYSGYVK YQLLSSYEAQ KRGGKGKLAV KTKEEDFIEN LLVANTHDII LCFSSKGILY 610 620 630 640 650 660 WMKVYQLPEA SRHARGRPIV NLLPLSSNER ITAILPISEY KDSINIFMAT SKGMVKKTSL 670 680 690 700 710 720 YEFKKPRTKG IIAINLKADD ELIGVSLTNG NNTIMLFTAQ GKAVHFSEIL VRKTGRTAIG 730 740 750 760 770 780 VQGIKIKKSD KVVSLVVPKK HGNILLITEH GYGKRTEIHE FPIKSRATQG TIAMKITKKN 790 800 810 820 830 840 GIVIGTMQVV NQDQIIIITN AGTLVRTRVL EIGILGRNTQ GVIIIRTSKK EKVVALQKAN ALHLNSV

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26902.1.
RefSeq	NP_777797.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Genome annotation databases
GeneID	1058401.
GenomeReviews	Gene locus bbp_169 in contig AE016826_GR.
KEGG	bab:bbp169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AS3.
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_169-MON.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_BUCBP

Accession

Primary (citable) accession number: Q89AS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)