Reviewed,
UniProtKB/Swiss-Prot Q89AR4 (AKH_BUCBP)
Last modified
February 9, 2010.
Version 51.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Bifunctional aspartokinase/homoserine dehydrogenase Short name=AK-HD Including the following 2 domains: 1- Recommended name: Aspartokinase EC=2.7.2.4 2- Recommended name: Homoserine dehydrogenase EC=1.1.1.3 | ||||
| Gene names |
| ||||
| Organism | Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 135842 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera |
Protein attributes
| Sequence length | 816 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H. ATP + L-aspartate = ADP + 4-phospho-L-aspartate. |
| Pathway | Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5. |
| Subunit structure | Homotetramer By similarity. |
| Sequence similarities | In the N-terminal section; belongs to the aspartokinase family. In the C-terminal section; belongs to the homoserine dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Threonine biosynthesis |
| Ligand | ATP-binding NADP Nucleotide-binding |
| Molecular function | Kinase Oxidoreductase Transferase |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH bindingInferred from electronic annotation. Source: InterPro aspartate kinase activityInferred from electronic annotation. Source: EC homoserine dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 816 | 816 | Bifunctional aspartokinase/homoserine dehydrogenase | PRO_0000066686 | |||||
Regions | |||||||||
| Nucleotide binding | 472 – 479 | 8 | NADP Potential | ||||||
| Region | 1 – 250 | 250 | Aspartokinase By similarity | ||||||
| Region | 251 – 471 | 221 | Interface By similarity | ||||||
| Region | 472 – 816 | 345 | Homoserine dehydrogenase By similarity | ||||||
Sequences
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References
| [1] | "Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE016826 Genomic DNA. Translation: AAO26915.1. |
| RefSeq | NP_777810.1. |
3D structure databases | |
| SMR | Q89AR4. Positions 3-463, 467-815. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1058230. |
| GenomeReviews | Gene locus bbp_183 in contig AE016826_GR. |
| KEGG | bab:bbp183. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG298754. |
| OMA | MFNVSGP. |
Enzyme and pathway databases | |
| BioCyc | BAPH224915:BBP_183-MONOMER. |
Family and domain databases | |
| InterPro | IPR001048. Asp/Glu/Uridylate_kinase. IPR005106. Asp/hSer_DH_NAD-bd. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. IPR011147. bifunc_aspartokin/hSer_DH. IPR001342. Homoserine_dehydrogenase_cat. IPR019811. Homoserine_dehydrogenase_CS. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF00696. AA_kinase. 1 hit. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view] |
| PIRSF | PIRSF000727. ThrA. 1 hit. |
| TIGRFAMs | TIGR00657. asp_kinases. 1 hit. |
| PROSITE | PS00324. ASPARTOKINASE. 1 hit. PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | AKH_BUCBP | ||||||||
| Accession | Primary (citable) accession number: Q89AR4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


