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Protein

Penicillin-binding protein 1B

Gene

mrcB

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Proton donor; for transglycosylase activityBy similarity
Active sitei454 – 4541Acyl-ester intermediate; for transpeptidase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-186-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1B
Short name:
PBP-1b
Short name:
PBP1b
Alternative name(s):
Murein polymerase
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.1.129)
Alternative name(s):
Peptidoglycan TGase
Peptidoglycan glycosyltransferase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcB
Ordered Locus Names:bbp_186
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
Proteomesi
  • UP000000601 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini34 – 741708ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741Penicillin-binding protein 1BPRO_0000083186Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224915.bbp186.

Structurei

3D structure databases

ProteinModelPortaliQ89AR2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 311173TransglycosylaseAdd
BLAST
Regioni395 – 687293TranspeptidaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
KOiK05365.
OMAiKDGYRYP.
OrthoDBiEOG6HMXCD.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011813. PBP_1b.
IPR001460. PCN-bd_Tpept.
IPR028166. UB2H.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
PF14814. UB2H. 1 hit.
[Graphical view]
PIRSFiPIRSF002799. PBP_1b. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02071. PBP_1b. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89AR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPVNLKLSI KFLFYFFLYF LLIIIIYGVY LYFKINQVIH GKIWKFPISI
60 70 80 90 100
YSRIVTLEPG NNYSKKDIIA ILKSNRYKQV NFLTMPGEFL VKRNSLILIR
110 120 130 140 150
RSFNFPEGFE DKISIKLLFD KNKLVRIVHL SNNRNFSILR LDPQLIAMIY
160 170 180 190 200
SPKGEKRLFV SQKNYPKALI QTLLTIEDKC FYNHYGINFY SMFRAFFVNL
210 220 230 240 250
ISGHSIQGGS TLTQQLVKNL FLTNIRSLWR KINEIYMALI LDFQYSKEKI
260 270 280 290 300
LELYLNEVYL GQDKNEQIRG FALASLYYFG RPINELRLDE CALLVGMVKG
310 320 330 340 350
ASLYNPWNNP VLTLNRRNLV LYVLFKHKVI NRTLYEKLKS KPLNIQSRGN
360 370 380 390 400
IIWFRSAFVQ IVEKEFQKKV GYYFQNFSGI KIFTTLDLIS QIAAENAIRH
410 420 430 440 450
GIQQLKKKYK LQDLEASMVI IDRFSGEIRG VLGSSNPNLL GYNRAIQAKR
460 470 480 490 500
SIGSLSKPIT YLAALSQPEY FRLNTWIPDT PIKIKMQNGK LWKPQNNNFE
510 520 530 540 550
FVGKVMLIDA LKNSMNVPIV HLSMKLGLKK IVQTWIQLGL SSNHIFKYPS
560 570 580 590 600
IALGSINLTS MEVAKIFQVI SSGGNKANII SIRSVLSENN KLIYHSFPQS
610 620 630 640 650
KQVISAQASY LTLYAMQSVV SSGTAKHLGK FFKNMHLAGK TGTTNNLVDS
660 670 680 690 700
WFVGIDGRQV VIVWIGRDNN KTTKCYGSTG AMKIYHNYLK LNNPKPLLLI
710 720 730 740
PPRDVYFLNI NKSGDFMCFR SYSKYFRAIP VWIRNHNIFC T
Length:741
Mass (Da):85,683
Last modified:June 1, 2003 - v1
Checksum:i950117AE79904004
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO26918.1.
RefSeqiWP_011091319.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO26918; AAO26918; bbp_186.
KEGGibab:bbp_186.
PATRICi21245147. VBIBucAph80364_0180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO26918.1.
RefSeqiWP_011091319.1. NC_004545.1.

3D structure databases

ProteinModelPortaliQ89AR2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224915.bbp186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO26918; AAO26918; bbp_186.
KEGGibab:bbp_186.
PATRICi21245147. VBIBucAph80364_0180.

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
KOiK05365.
OMAiKDGYRYP.
OrthoDBiEOG6HMXCD.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciBAPH224915:GJ9D-186-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011813. PBP_1b.
IPR001460. PCN-bd_Tpept.
IPR028166. UB2H.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
PF14814. UB2H. 1 hit.
[Graphical view]
PIRSFiPIRSF002799. PBP_1b. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02071. PBP_1b. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiPBPB_BUCBP
AccessioniPrimary (citable) accession number: Q89AR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: June 1, 2003
Last modified: March 16, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.