Reviewed, UniProtKB/Swiss-Prot Q89AQ7 (MTNN_BUCBP)

Names and origin

Protein names

Recommended name:
    5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
      Short name=MTA/SAH nucleosidase
      Short name=MTAN
    EC=3.2.2.9
Alternative name(s):
    5'-methylthioadenosine nucleosidase
      Short name=MTA nucleosidase
    S-adenosylhomocysteine nucleosidase
      Short name=SAH nucleosidase
      Short name=AdoHcy nucleosidase
      Short name=SRH nucleosidase

Gene names

Name:	mtnN
Synonyms:	pfs
Ordered Locus Names:	bbp_192

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity.
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684 S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP methionine salvage Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: HAMAP methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684		PRO_0000164438
Regions
	Region	181 – 182	2	Substrate binding By similarity
Sites
	Active site	20	1	Proton acceptor By similarity
	Binding site	86	1	Substrate; via amide nitrogen By similarity
	Binding site	160	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	205	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AQ7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F0D0803BBB38EE7F	FASTA	252	28,426
	10 20 30 40 50 60 MKKEIKKNYR IGIIAALQQE VQILFNKLKN YKINKISNIT FYIGNIHNIH VVLAKSGVGK 70 80 90 100 110 120 VFSGITCALL LQKYKVKFII NIGSAGSLNK NLKPGSIIIP TNVCYHDVNL TAFGYSIGQI 130 140 150 160 170 180 KNCPKTFLSN TLMLKLTEKY LFENKIKYQK KLMISGDIFI DTCEKKSLLK KRFPKAIAVD 190 200 210 220 230 240 MEAAAIAHVC YQFNIPILII KSISDSSDIN AADNFKYFIN LASKNSSLVT INVLQTLFKN 250 TKNILFDNNN RC

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26924.1.
RefSeq	NP_777819.1.
3D structure databases
HSSP	HSSP built from PDB template 1JYS based on UniProtKB P24247.
ModBase	Search...
Genome annotation databases
GeneID	1058386.
GenomeReviews	Gene locus bbp_192 in contig AE016826_GR.
KEGG	bab:bbp192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AQ7.
OMA	AQVCHQF.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_192-MON.
Family and domain databases
HAMAP	MF_01684. [Tree]
InterPro	IPR010049. MTA_SAH_Nsdase. IPR000845. Nucleoside_phosphorylase. IPR018017. Nucleoside_phosphorylase_1. [Graphical view]
PANTHER	PTHR21234. PNP_UDP. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNN_BUCBP

Accession

Primary (citable) accession number: Q89AQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)