Reviewed, UniProtKB/Swiss-Prot Q89AQ2 (MURD_BUCBP)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	bbp_200

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108985

Sequences

	Sequence		Length	Mass (Da)
	Q89AQ2-1 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: C4876568C23CE357	FASTA	442	50,066
	10 20 30 40 50 60 MTRNYLHKKI LIFGMGLTGI SCLNFFLSKG IYPKIMDTDK RPKHIEKIIK FKNICYHTGS 70 80 90 100 110 120 VNYSWILQSN LIIVSPGITP SHPALKFATK KNIEIIGDIE LFVQETKVPI IAITGSNGKS 130 140 150 160 170 180 SVTKIVKEII QKAGFTTYIG GNIGIPALNI VNKFAHFFIL ELSSFQLERT FSLKAYIATI 190 200 210 220 230 240 LNITPDHLNR YSSDIKEYEK AKQKIYKNSK ICIINVDNPV TINRQAQLTK CISFGVHSGD 250 260 270 280 290 300 YHLSHTYTNT WLCYKSLKLI NTKKLKLSGR HNYINMLSAL AIVHELKISF KISVRILKNF 310 320 330 340 350 360 LGLPHRCQKV YKNNNITWIN DSKSTNIAST KSAIQSINTK GKIRLILGGD KKSSNLNLLK 370 380 390 400 410 420 PILKNNAIVI YCYGKDKKEL FNLYPHKSKI FETLQEVMQH ISVQVQPGDV VLLSPACSSL 430 440 DQFSGFEERG NTFVKLIQEL IH

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26932.1.
RefSeq	NP_777827.1.
3D structure databases
HSSP	HSSP built from PDB template 4UAG based on UniProtKB P14900.
ModBase	Search...
Genome annotation databases
GeneID	1058151.
GenomeReviews	Gene locus bbp_200 in contig AE016826_GR.
KEGG	bab:bbp200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AQ2.
OMA	Q89AQ2. DSKATNF.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_200-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BUCBP

Accession

Primary (citable) accession number: Q89AQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)