Reviewed, UniProtKB/Swiss-Prot Q89AP3 (AMPM_BUCBP)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	bbp_212

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Methionine aminopeptidase		PRO_0000148931
Sites
	Metal binding	98	1	Cobalt 1 By similarity
	Metal binding	109	1	Cobalt 1 By similarity
	Metal binding	109	1	Cobalt 2 By similarity
	Metal binding	172	1	Cobalt 2 By similarity
	Metal binding	206	1	Cobalt 2 By similarity
	Metal binding	237	1	Cobalt 1 By similarity
	Metal binding	237	1	Cobalt 2 By similarity
	Binding site	80	1	Substrate By similarity
	Binding site	179	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AP3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 564C2FC926EEBCA6	FASTA	266	30,049
	10 20 30 40 50 60 MTCIFIKNIN EINKMRLVGK LVADVLDMIK EYIVPGITTE ELNNICHNYI TYKQHAKPAC 70 80 90 100 110 120 LGYQGFPKSI CTSINDIVCH GIPNKNSILK NGDIINIDVA ILKDKYYSDA SKMFFVGKPT 130 140 150 160 170 180 ELGKKLCYVA KKSLYLALYT IRPGINLQKL GKVIQNYVKK QNFSIVKEYC GHGIGRSFHE 190 200 210 220 230 240 PPQILHHNYY KSNTTILQSG MTFTVEPMIN SGSCEVQCTN DGWTVKTKDK SLSAQYEHTI 250 260 LVNEEGCEIL TLQKGEQISR ILKNLT

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26944.1.
RefSeq	NP_777839.1.
3D structure databases
HSSP	HSSP built from PDB template 1MAT based on UniProtKB P07906.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Genome annotation databases
GeneID	1058483.
GenomeReviews	Gene locus bbp_212 in contig AE016826_GR.
KEGG	bab:bbp212.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AP3.
OMA	VIQKHAE.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_212-MON.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_BUCBP

Accession

Primary (citable) accession number: Q89AP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)