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Q89AP3

- MAP1_BUCBP

UniProt

Q89AP3 - MAP1_BUCBP

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Protein

Methionine aminopeptidase

Gene

map

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801SubstrateUniRule annotation
Metal bindingi98 – 981Divalent metal cation 1UniRule annotation
Metal bindingi109 – 1091Divalent metal cation 1UniRule annotation
Metal bindingi109 – 1091Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi172 – 1721Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotation
Metal bindingi206 – 2061Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi237 – 2371Divalent metal cation 1UniRule annotation
Metal bindingi237 – 2371Divalent metal cation 2; catalyticUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-212-MONOMER.

Protein family/group databases

MEROPSiM24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:bbp_212
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000601: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Methionine aminopeptidasePRO_0000148931Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224915.bbp212.

Structurei

3D structure databases

ProteinModelPortaliQ89AP3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0024.
KOiK01265.
OMAiEGMCFTI.
OrthoDBiEOG6MWNDS.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89AP3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTCIFIKNIN EINKMRLVGK LVADVLDMIK EYIVPGITTE ELNNICHNYI
60 70 80 90 100
TYKQHAKPAC LGYQGFPKSI CTSINDIVCH GIPNKNSILK NGDIINIDVA
110 120 130 140 150
ILKDKYYSDA SKMFFVGKPT ELGKKLCYVA KKSLYLALYT IRPGINLQKL
160 170 180 190 200
GKVIQNYVKK QNFSIVKEYC GHGIGRSFHE PPQILHHNYY KSNTTILQSG
210 220 230 240 250
MTFTVEPMIN SGSCEVQCTN DGWTVKTKDK SLSAQYEHTI LVNEEGCEIL
260
TLQKGEQISR ILKNLT
Length:266
Mass (Da):30,049
Last modified:June 1, 2003 - v1
Checksum:i564C2FC926EEBCA6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26944.1.
RefSeqiNP_777839.1. NC_004545.1.
WP_011091345.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO26944; AAO26944; bbp_212.
GeneIDi1058483.
KEGGibab:bbp212.
PATRICi21245199. VBIBucAph80364_0206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26944.1 .
RefSeqi NP_777839.1. NC_004545.1.
WP_011091345.1. NC_004545.1.

3D structure databases

ProteinModelPortali Q89AP3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224915.bbp212.

Protein family/group databases

MEROPSi M24.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO26944 ; AAO26944 ; bbp_212 .
GeneIDi 1058483.
KEGGi bab:bbp212.
PATRICi 21245199. VBIBucAph80364_0206.

Phylogenomic databases

eggNOGi COG0024.
KOi K01265.
OMAi EGMCFTI.
OrthoDBi EOG6MWNDS.

Enzyme and pathway databases

BioCyci BAPH224915:GJ9D-212-MONOMER.

Family and domain databases

Gene3Di 3.90.230.10. 1 hit.
HAMAPi MF_01974. MetAP_1.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 1 hit.
TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
PROSITEi PS00680. MAP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiMAP1_BUCBP
AccessioniPrimary (citable) accession number: Q89AP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: October 1, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3