Reviewed, UniProtKB/Swiss-Prot Q89AM6 (LEP_BUCBP)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	bbp_240

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Signal peptidase I		PRO_0000109505
Regions
	Transmembrane	5 – 25	21	Potential
	Topological domain	26 – 57	32	Cytoplasmic Potential
	Transmembrane	58 – 78	21	Potential
	Topological domain	79 – 310	232	Extracellular Potential
Sites
	Active site	82	1	By similarity
	Active site	137	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AM6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6CB12207E9CF21B8	FASTA	310	36,313
	10 20 30 40 50 60 MSNYLSSFLL ASSLITGTLW IINKILSHNL LDSKIPFNIK KSKIYYKSKQ VVQTFASFFP 70 80 90 100 110 120 ILIIVFIIRT FICEPFQIPS ESMMPTLLPG DFILVKKFSY GIKNPFSNNV IVFINTPKRG 130 140 150 160 170 180 DIVVFKHPNN NAINYVKRIV GLPGDKINYN ILTKRLTITP NNINEQHTKN ISINYKYIKP 190 200 210 220 230 240 NDFTKHFKLN NIILNNVHSL ESSNNNLLQL EMYQEKIEKI AYNIFFKKKL IDQKDLYFKQ 250 260 270 280 290 300 FSQKQGTWIV PKHKYFVLGD NRDNSLDSRY WGFVPEKNLI GKVVFIWMHL IKKEGQWPTG 310 IQFDRIGNIY

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26967.1.
RefSeq	NP_777862.1.
3D structure databases
HSSP	HSSP built from PDB template 1KN9 based on UniProtKB P00803.
ModBase	Search...
Protein family/group databases
MEROPS	S26.001.
Genome annotation databases
GeneID	1058242.
GenomeReviews	Gene locus bbp_240 in contig AE016826_GR.
KEGG	bab:bbp240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AM6.
OMA	Q89AM6. VAVFKYP.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_240-MON.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR019533. Peptidase_S26_cons-reg. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. PF10502. Peptidase_S26. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_BUCBP

Accession

Primary (citable) accession number: Q89AM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)