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Q89AM1

- FABI_BUCBP

UniProt

Q89AM1 - FABI_BUCBP

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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene
fabI, bbp_246
Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis By similarity.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygen By similarity
Binding sitei92 – 921NAD; via carbonyl oxygen By similarity
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygen By similarity
Active sitei146 – 1461Proton acceptor By similarity
Active sitei156 – 1561Proton acceptor By similarity
Binding sitei163 – 1631NAD By similarity
Sitei201 – 2011Involved in acyl-ACP binding By similarity
Sitei204 – 2041Involved in acyl-ACP binding By similarity
Sitei205 – 2051Involved in acyl-ACP binding By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NAD By similarity
Nucleotide bindingi64 – 652NAD By similarity
Nucleotide bindingi192 – 1943NAD By similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB
  2. fatty acid elongation Source: UniProtKB
  3. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-246-MONOMER.
UniPathwayiUPA00078.
UPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:bbp_246
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000601: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054898Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi224915.bbp246.

Structurei

3D structure databases

ProteinModelPortaliQ89AM1.
SMRiQ89AM1. Positions 2-257.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiGISGEIM.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89AM1-1 [UniParc]FASTAAdd to Basket

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MGFLEEKKIL VTGISNKYSI AFGIAKALHK QNATLAFSYH TDRLKNKVYE    50
LAKELGVKIV IPCDVSDDNS IKRLFFNISK KWITFDGFIH SIAFAPKNQL 100
SGDYVSSITR LDFSNVLDVS SYSFVGMAKA CRSILKKGSS LLTLSYIGSK 150
KVVPNYNVMG IAKASLESNV RYMASCMGLN GIRVNAISSS PIKTLSSYHI 200
KNFKKILNHT TSRSLNNNLT TVEDVGNTAA FLCSDLSKGI TGQIIYVDGG 250
FNITAMSNSE 260
Length:260
Mass (Da):28,500
Last modified:May 30, 2003 - v1
Checksum:i34E231597C22B560
GO

Sequence cautioni

The sequence AAO26973.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26973.1. Different initiation.
RefSeqiNP_777868.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO26973; AAO26973; bbp_246.
GeneIDi1058250.
KEGGibab:bbp246.
PATRICi21245273. VBIBucAph80364_0239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26973.1 . Different initiation.
RefSeqi NP_777868.1. NC_004545.1.

3D structure databases

ProteinModelPortali Q89AM1.
SMRi Q89AM1. Positions 2-257.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224915.bbp246.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO26973 ; AAO26973 ; bbp_246 .
GeneIDi 1058250.
KEGGi bab:bbp246.
PATRICi 21245273. VBIBucAph80364_0239.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi GISGEIM.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00078 .
UPA00094 .
BioCyci BAPH224915:GJ9D-246-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiFABI_BUCBP
AccessioniPrimary (citable) accession number: Q89AM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: May 30, 2003
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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