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Q89AM1

- FABI_BUCBP

UniProt

Q89AM1 - FABI_BUCBP

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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
Binding sitei92 – 921NAD; via carbonyl oxygenBy similarity
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygenBy similarity
Active sitei146 – 1461Proton acceptorBy similarity
Active sitei156 – 1561Proton acceptorBy similarity
Binding sitei163 – 1631NADBy similarity
Sitei201 – 2011Involved in acyl-ACP bindingBy similarity
Sitei204 – 2041Involved in acyl-ACP bindingBy similarity
Sitei205 – 2051Involved in acyl-ACP bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NADBy similarity
Nucleotide bindingi64 – 652NADBy similarity
Nucleotide bindingi192 – 1943NADBy similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB
  2. fatty acid elongation Source: UniProtKB
  3. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-246-MONOMER.
UniPathwayiUPA00078.
UPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:bbp_246
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000601: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054898Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi224915.bbp246.

Structurei

3D structure databases

ProteinModelPortaliQ89AM1.
SMRiQ89AM1. Positions 2-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiGISGEIM.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89AM1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGFLEEKKIL VTGISNKYSI AFGIAKALHK QNATLAFSYH TDRLKNKVYE
60 70 80 90 100
LAKELGVKIV IPCDVSDDNS IKRLFFNISK KWITFDGFIH SIAFAPKNQL
110 120 130 140 150
SGDYVSSITR LDFSNVLDVS SYSFVGMAKA CRSILKKGSS LLTLSYIGSK
160 170 180 190 200
KVVPNYNVMG IAKASLESNV RYMASCMGLN GIRVNAISSS PIKTLSSYHI
210 220 230 240 250
KNFKKILNHT TSRSLNNNLT TVEDVGNTAA FLCSDLSKGI TGQIIYVDGG
260
FNITAMSNSE
Length:260
Mass (Da):28,500
Last modified:May 30, 2003 - v1
Checksum:i34E231597C22B560
GO

Sequence cautioni

The sequence AAO26973.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26973.1. Different initiation.
RefSeqiNP_777868.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO26973; AAO26973; bbp_246.
GeneIDi1058250.
KEGGibab:bbp246.
PATRICi21245273. VBIBucAph80364_0239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016826 Genomic DNA. Translation: AAO26973.1 . Different initiation.
RefSeqi NP_777868.1. NC_004545.1.

3D structure databases

ProteinModelPortali Q89AM1.
SMRi Q89AM1. Positions 2-257.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224915.bbp246.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO26973 ; AAO26973 ; bbp_246 .
GeneIDi 1058250.
KEGGi bab:bbp246.
PATRICi 21245273. VBIBucAph80364_0239.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi GISGEIM.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00078 .
UPA00094 .
BioCyci BAPH224915:GJ9D-246-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiFABI_BUCBP
AccessioniPrimary (citable) accession number: Q89AM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: May 30, 2003
Last modified: October 1, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3