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Q89AM1

- FABI_BUCBP

UniProt

Q89AM1 - FABI_BUCBP

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (30 May 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis By similarity.By similarity

    Catalytic activityi

    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
    Binding sitei92 – 921NAD; via carbonyl oxygenBy similarity
    Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygenBy similarity
    Active sitei146 – 1461Proton acceptorBy similarity
    Active sitei156 – 1561Proton acceptorBy similarity
    Binding sitei163 – 1631NADBy similarity
    Sitei201 – 2011Involved in acyl-ACP bindingBy similarity
    Sitei204 – 2041Involved in acyl-ACP bindingBy similarity
    Sitei205 – 2051Involved in acyl-ACP bindingBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 202NADBy similarity
    Nucleotide bindingi64 – 652NADBy similarity
    Nucleotide bindingi192 – 1943NADBy similarity

    GO - Molecular functioni

    1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB
    2. fatty acid elongation Source: UniProtKB
    3. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciBAPH224915:GJ9D-246-MONOMER.
    UniPathwayiUPA00078.
    UPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
    Short name:
    ENR
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase
    Gene namesi
    Name:fabI
    Ordered Locus Names:bbp_246
    OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
    Taxonomic identifieri224915 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000601: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 260260Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054898Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi224915.bbp246.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89AM1.
    SMRiQ89AM1. Positions 2-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0623.
    KOiK00208.
    OMAiGISGEIM.
    OrthoDBiEOG6HF644.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q89AM1-1 [UniParc]FASTAAdd to Basket

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    MGFLEEKKIL VTGISNKYSI AFGIAKALHK QNATLAFSYH TDRLKNKVYE    50
    LAKELGVKIV IPCDVSDDNS IKRLFFNISK KWITFDGFIH SIAFAPKNQL 100
    SGDYVSSITR LDFSNVLDVS SYSFVGMAKA CRSILKKGSS LLTLSYIGSK 150
    KVVPNYNVMG IAKASLESNV RYMASCMGLN GIRVNAISSS PIKTLSSYHI 200
    KNFKKILNHT TSRSLNNNLT TVEDVGNTAA FLCSDLSKGI TGQIIYVDGG 250
    FNITAMSNSE 260
    Length:260
    Mass (Da):28,500
    Last modified:May 30, 2003 - v1
    Checksum:i34E231597C22B560
    GO

    Sequence cautioni

    The sequence AAO26973.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016826 Genomic DNA. Translation: AAO26973.1. Different initiation.
    RefSeqiNP_777868.1. NC_004545.1.

    Genome annotation databases

    EnsemblBacteriaiAAO26973; AAO26973; bbp_246.
    GeneIDi1058250.
    KEGGibab:bbp246.
    PATRICi21245273. VBIBucAph80364_0239.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016826 Genomic DNA. Translation: AAO26973.1 . Different initiation.
    RefSeqi NP_777868.1. NC_004545.1.

    3D structure databases

    ProteinModelPortali Q89AM1.
    SMRi Q89AM1. Positions 2-257.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224915.bbp246.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO26973 ; AAO26973 ; bbp_246 .
    GeneIDi 1058250.
    KEGGi bab:bbp246.
    PATRICi 21245273. VBIBucAph80364_0239.

    Phylogenomic databases

    eggNOGi COG0623.
    KOi K00208.
    OMAi GISGEIM.
    OrthoDBi EOG6HF644.

    Enzyme and pathway databases

    UniPathwayi UPA00078 .
    UPA00094 .
    BioCyci BAPH224915:GJ9D-246-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bp.

    Entry informationi

    Entry nameiFABI_BUCBP
    AccessioniPrimary (citable) accession number: Q89AM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2003
    Last sequence update: May 30, 2003
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3