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Q89AK5 (BIOB_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:bbp_272
OrganismBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]
Taxonomic identifier224915 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Biotin synthase HAMAP-Rule MF_01694
PRO_0000185549

Sites

Metal binding511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding951Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1861Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2581Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89AK5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5EE82C8B72164DCC

FASTA34439,319
        10         20         30         40         50         60 
MKTQWNFEKV KELFDQPFFD ILFLAQNIHR KNFNANQIQI STLLSIKTGA CPEDCKYCPQ 

        70         80         90        100        110        120 
SARYKTNIKI EKLLTLKQIL KSAQQAKKLG STRFCMGAAW KNPKERDMHF LKTVIQEVKK 

       130        140        150        160        170        180 
LGLETCMTLG TLHNDQADRL AKAGLDFYNH NLDTSKSYYQ KIVTTRTYQD RLNTLKKVRK 

       190        200        210        220        230        240 
SGMKICSGGI LGLGEKLKDR IELLIELSNL EIAPESIPIN MLVKVKGTPL ENQTSIDTFD 

       250        260        270        280        290        300 
FIKTIAITRI MMPTSYIRLS AGRENMNEQT QAMCFLSGAN SIFYGCKLLT TPNPKKERDK 

       310        320        330        340 
QLFSKLGLNI KHKNTNLNYS ILSDNSLNYT SKIHSQQFYN AETA 

« Hide

References

[1]"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016826 Genomic DNA. Translation: AAO26997.1.
RefSeqNP_777892.1. NC_004545.1.

3D structure databases

ProteinModelPortalQ89AK5.
SMRQ89AK5. Positions 2-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224915.bbp272.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO26997; AAO26997; bbp_272.
GeneID1058249.
KEGGbab:bbp272.
PATRIC21245329. VBIBucAph80364_0267.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycBAPH224915:GJ9D-272-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_BUCBP
AccessionPrimary (citable) accession number: Q89AK5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways