Reviewed, UniProtKB/Swiss-Prot Q89AJ2 (TRXB_BUCBP)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
      Short name=TRXR
    EC=1.8.1.9

Gene names

Name:	trxB
Ordered Locus Names:	bbp_291

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Thioredoxin reductase		PRO_0000166723
Regions
	Nucleotide binding	40 – 47	8	FAD By similarity
	Nucleotide binding	291 – 300	10	FAD By similarity
Amino acid modifications
	Disulfide bond	141 ↔ 144		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AJ2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 47CCA57337B0A069	FASTA	326	35,695
	10 20 30 40 50 60 MIENTLKIEH HKLIILGSGP AGYTAAIYSA RANLEPLLFT GNNKGGQLIN TNEIENWPGD 70 80 90 100 110 120 SKSLTGLELM ERMHNHAKSL NTKIIPNEII RVNFFKIPFL LVSDTNQYYT SDSVIIATGA 130 140 150 160 170 180 SPKYLGLTSE SKFIGKGVSV CAVCDGFFYK NEDVAVVGGG NTALEEALYL SNIARKVYLI 190 200 210 220 230 240 HRRKTFSAEK ILISRMLNKT KKNIILRTGC IVNKIIGGVN GVRGVQITCN DSKENKCLIN 250 260 270 280 290 300 LSGVFIAIGH APNTKLFKNQ LFMKNDYILV KSGIHGNVTQ TNIPGIFAAG DVIDHVYKQA 310 320 ITSSASGCMA ALDAEKYIDQ KYGFKI

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO27016.1.
RefSeq	NP_777911.1.
3D structure databases
HSSP	HSSP built from PDB template 1TRB based on UniProtKB P09625.
ModBase	Search...
Genome annotation databases
GeneID	1058461.
GenomeReviews	Gene locus bbp_291 in contig AE016826_GR.
KEGG	bab:bbp291.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AJ2.
OMA	Q89AJ2. WDSAIDE.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_291-MON.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_BUCBP

Accession

Primary (citable) accession number: Q89AJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)