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Q89AI8 (KPYK_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pykA
Ordered Locus Names:bbp_297
OrganismBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]
Taxonomic identifier224915 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Enzyme regulation

Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Pyruvate kinase
PRO_0000112060

Sites

Metal binding381Potassium By similarity
Metal binding401Potassium By similarity
Metal binding701Potassium By similarity
Metal binding2251Magnesium By similarity
Metal binding2521Magnesium By similarity
Binding site361Substrate By similarity
Binding site2511Substrate; via amide nitrogen By similarity
Binding site2521Substrate; via amide nitrogen By similarity
Binding site2841Substrate By similarity
Site2231Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89AI8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 674367EACE323020

FASTA47952,698
        10         20         30         40         50         60 
MIRRFRRTKI VATLGPSTDN HIILENMIKS GVNVFRLNFS HGTREEHIYR AKLVTSIAKR 

        70         80         90        100        110        120 
LNCHIALLGD LQGPKIRICK FQDKKVFLKV NNYFILDANL NENLGTSERV GIDYKNLPKD 

       130        140        150        160        170        180 
VKKCDILLLD DGKIQLKVIN IVDQEIFTKI IIGGVLSNNK GINKLGGGLS ARILTKKDKR 

       190        200        210        220        230        240 
DIITSIDIGV DYLAVSFPRY GSDLDHARKC AVKFGSKAKI IAKIERAEAV KDLKIIDEII 

       250        260        270        280        290        300 
LASDAIMVAR GDLGVEIGES ELAGIQKTLI RRARQLNRVV ITATQMMESM INNPMPTRAE 

       310        320        330        340        350        360 
VMDVANAVLD GSDAVMLSAE TASGKYPIET IKVMSKVCMG AEKMPSINVS QHRIHDKFHD 

       370        380        390        400        410        420 
IEEAIAMSAM YAANHLSGIT AIITMTESGK TSLMTSRITS GLPIFALSSH IQTLKLTALY 

       430        440        450        460        470 
RGVTPIFFNS SKEGVSAANE VINLLVKRGF LEPGNLVIIT QGDIMGKVGK TNTSRILKV 

« Hide

References

[1]"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016826 Genomic DNA. Translation: AAO27022.1.
RefSeqNP_777917.1. NC_004545.1.

3D structure databases

ProteinModelPortalQ89AI8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224915.bbp297.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO27022; AAO27022; bbp_297.
GeneID1058264.
KEGGbab:bbp297.
PATRIC21245381. VBIBucAph80364_0293.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0469.
KOK00873.
OMATPVYFDS.
OrthoDBEOG6GBMB0.

Enzyme and pathway databases

BioCycBAPH224915:GJ9D-297-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_BUCBP
AccessionPrimary (citable) accession number: Q89AI8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: November 13, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways