Q89AI8 (KPYK_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:	pykA
Ordered Locus Names:	bbp_297

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Baizongia pistaciae)

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Enzyme regulation	Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Pyruvate kinase		PRO_0000112060
Sites
	Metal binding	38	1	Potassium By similarity
	Metal binding	40	1	Potassium By similarity
	Metal binding	70	1	Potassium By similarity
	Metal binding	225	1	Magnesium By similarity
	Metal binding	252	1	Magnesium By similarity
	Binding site	36	1	Substrate By similarity
	Binding site	251	1	Substrate; via amide nitrogen By similarity
	Binding site	252	1	Substrate; via amide nitrogen By similarity
	Binding site	284	1	Substrate By similarity
	Site	223	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AI8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 674367EACE323020	FASTA	479	52,698
	10 20 30 40 50 60 MIRRFRRTKI VATLGPSTDN HIILENMIKS GVNVFRLNFS HGTREEHIYR AKLVTSIAKR 70 80 90 100 110 120 LNCHIALLGD LQGPKIRICK FQDKKVFLKV NNYFILDANL NENLGTSERV GIDYKNLPKD 130 140 150 160 170 180 VKKCDILLLD DGKIQLKVIN IVDQEIFTKI IIGGVLSNNK GINKLGGGLS ARILTKKDKR 190 200 210 220 230 240 DIITSIDIGV DYLAVSFPRY GSDLDHARKC AVKFGSKAKI IAKIERAEAV KDLKIIDEII 250 260 270 280 290 300 LASDAIMVAR GDLGVEIGES ELAGIQKTLI RRARQLNRVV ITATQMMESM INNPMPTRAE 310 320 330 340 350 360 VMDVANAVLD GSDAVMLSAE TASGKYPIET IKVMSKVCMG AEKMPSINVS QHRIHDKFHD 370 380 390 400 410 420 IEEAIAMSAM YAANHLSGIT AIITMTESGK TSLMTSRITS GLPIFALSSH IQTLKLTALY 430 440 450 460 470 RGVTPIFFNS SKEGVSAANE VINLLVKRGF LEPGNLVIIT QGDIMGKVGK TNTSRILKV

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27022.1.
RefSeq	NP_777917.1. NC_004545.1.
3D structure databases
ProteinModelPortal	Q89AI8.
ModBase	Search...
Protein-protein interaction databases
STRING	224915.bbp297.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO27022; AAO27022; bbp_297.
GeneID	1058264.
KEGG	bab:bbp297.
PATRIC	21245381. VBIBucAph80364_0293.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0469.
KO	K00873.
OMA	SHTICIA.
Enzyme and pathway databases
BioCyc	BAPH224915:GJ9D-297-MONOMER.
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_BUCBP

Accession

Primary (citable) accession number: Q89AI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program