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Protein

Pyruvate kinase

Gene

pykA

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Enzyme regulationi

Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK (By similarity).By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Metal bindingi38PotassiumBy similarity1
Metal bindingi40PotassiumBy similarity1
Metal bindingi70PotassiumBy similarity1
Sitei223Transition state stabilizerBy similarity1
Metal bindingi225MagnesiumBy similarity1
Binding sitei251Substrate; via amide nitrogenBy similarity1
Metal bindingi252MagnesiumBy similarity1
Binding sitei252Substrate; via amide nitrogenBy similarity1
Binding sitei284SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciBAPH224915:G1G09-301-MONOMER
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pykA
Ordered Locus Names:bbp_297
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000601 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120601 – 479Pyruvate kinaseAdd BLAST479

Proteomic databases

PRIDEiQ89AI8

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi224915.bbp297

Structurei

3D structure databases

ProteinModelPortaliQ89AI8
SMRiQ89AI8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
KOiK00873
OMAiICVEAEK
OrthoDBiPOG091H02AT

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q89AI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRRFRRTKI VATLGPSTDN HIILENMIKS GVNVFRLNFS HGTREEHIYR
60 70 80 90 100
AKLVTSIAKR LNCHIALLGD LQGPKIRICK FQDKKVFLKV NNYFILDANL
110 120 130 140 150
NENLGTSERV GIDYKNLPKD VKKCDILLLD DGKIQLKVIN IVDQEIFTKI
160 170 180 190 200
IIGGVLSNNK GINKLGGGLS ARILTKKDKR DIITSIDIGV DYLAVSFPRY
210 220 230 240 250
GSDLDHARKC AVKFGSKAKI IAKIERAEAV KDLKIIDEII LASDAIMVAR
260 270 280 290 300
GDLGVEIGES ELAGIQKTLI RRARQLNRVV ITATQMMESM INNPMPTRAE
310 320 330 340 350
VMDVANAVLD GSDAVMLSAE TASGKYPIET IKVMSKVCMG AEKMPSINVS
360 370 380 390 400
QHRIHDKFHD IEEAIAMSAM YAANHLSGIT AIITMTESGK TSLMTSRITS
410 420 430 440 450
GLPIFALSSH IQTLKLTALY RGVTPIFFNS SKEGVSAANE VINLLVKRGF
460 470
LEPGNLVIIT QGDIMGKVGK TNTSRILKV
Length:479
Mass (Da):52,698
Last modified:June 1, 2003 - v1
Checksum:i674367EACE323020
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA Translation: AAO27022.1
RefSeqiWP_011091423.1, NC_004545.1

Genome annotation databases

EnsemblBacteriaiAAO27022; AAO27022; bbp_297
KEGGibab:bbp_297

Similar proteinsi

Entry informationi

Entry nameiKPYK_BUCBP
AccessioniPrimary (citable) accession number: Q89AI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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