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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381NADPBy similarity
Metal bindingi51 – 511Calcium 1; via carbonyl oxygen; shared with dimeric partnerBy similarity
Metal bindingi54 – 541Calcium 1; via carbonyl oxygen; shared with dimeric partnerBy similarity
Binding sitei87 – 871NADP; via carbonyl oxygenBy similarity
Binding sitei139 – 1391SubstrateBy similarity
Metal bindingi146 – 1461Calcium 2By similarity
Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotation
Binding sitei185 – 1851NADP; via amide nitrogen and carbonyl oxygenBy similarity
Metal bindingi234 – 2341Calcium 3; shared with dimeric partnerBy similarity
Metal bindingi235 – 2351Calcium 3; via carbonyl oxygen; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 154NADPBy similarity
Nucleotide bindingi60 – 612NADPBy similarity
Nucleotide bindingi152 – 1565NADPBy similarity

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding, NADP

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-321-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:bbp_321
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000601: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2452453-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054668Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi224915.bbp321.

Structurei

3D structure databases

ProteinModelPortaliQ89AG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK00059.
OMAiVFVHENT.
OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89AG9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTTKKIAVI TGANRGLGKG IAEELSNTNN ITVIGTSTSQ KGCKIINKYL
60 70 80 90 100
KNNGIGIKLD ITNPNEITKT MDFVYKNFGR VDILINNAGI IRDKLLINMK
110 120 130 140 150
TQDWNSVLNV NLNSIFYMSK SVIRNMIKNK QGKIITIGSV IAHIGNCGQT
160 170 180 190 200
NYSAAKLGLV GFHKSLALEL APKGITVNMI APGLIKTGMT NNLSQKQLSK
210 220 230 240
YLSKIPMKRL GTIKEISKIT LFLISNDANY ITGQVIHVNG GMYMP
Length:245
Mass (Da):26,788
Last modified:May 30, 2003 - v1
Checksum:i6B94C5520D69157E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO27043.1.
RefSeqiNP_777938.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO27043; AAO27043; bbp_321.
GeneIDi1058479.
KEGGibab:bbp321.
PATRICi21245431. VBIBucAph80364_0315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO27043.1.
RefSeqiNP_777938.1. NC_004545.1.

3D structure databases

ProteinModelPortaliQ89AG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224915.bbp321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO27043; AAO27043; bbp_321.
GeneIDi1058479.
KEGGibab:bbp321.
PATRICi21245431. VBIBucAph80364_0315.

Phylogenomic databases

eggNOGiCOG1028.
KOiK00059.
OMAiVFVHENT.
OrthoDBiEOG6N3CR8.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciBAPH224915:GJ9D-321-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiFABG_BUCBP
AccessioniPrimary (citable) accession number: Q89AG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: May 30, 2003
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Calcium ions stabilize the structure, and may inhibit FabG activity by obstructing access to the active site.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.