Reviewed, UniProtKB/Swiss-Prot Q89AG6 (PTGCB_BUCBP)

Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIICB component
Alternative name(s):
    EIICB-Glc
      Short name=EII-Glc
Including the following 2 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component

Gene names

Name:	ptsG
Ordered Locus Names:	bbp_326

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose transport Inferred from electronic annotation. Source: InterPro phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	PTS system glucose-specific EIICB component		PRO_0000186527
Regions
	Transmembrane	15 – 35	21	Potential
	Transmembrane	51 – 71	21	Potential
	Transmembrane	80 – 100	21	Potential
	Transmembrane	112 – 132	21	Potential
	Transmembrane	152 – 172	21	Potential
	Transmembrane	252 – 272	21	Potential
	Transmembrane	280 – 300	21	Potential
	Transmembrane	305 – 325	21	Potential
	Transmembrane	356 – 376	21	Potential
	Domain	1 – 388	388	PTS EIIC type-1
	Domain	399 – 479	81	PTS EIIB type-1
Sites
	Active site	421	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AG6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: FF1B60760B4D9ABD	FASTA	479	52,157
	10 20 30 40 50 60 MFKNAFSNLQ KIGRSLMLPV SVLPIAGILL GIGSANFKII PHTISNIMTE AGSSVFSNMP 70 80 90 100 110 120 LIFAIGIALG FTKNDGVSAL AAVVSYGIMT KTLSITIPIF SNLSDINVNQ KYLLDTGILG 130 140 150 160 170 180 GIIAGSISAY IFNTFYRIQL PEYLGFFAGR RFVPIASGLI SIIFGCILSI IWPPIGNVIK 190 200 210 220 230 240 TFSEWAAYQN PTLAFGIYGT VERALVPFGL HHIWNVPFQM QIGEYNNSIG QIFHGDIARY 250 260 270 280 290 300 MAGDSTAGKL SGGFIFKMYG LPFAALAMWH CANKKNKAKI GGIMMSGALT AILTGITEPI 310 320 330 340 350 360 EFSFILVAPI LYVIHAILAG LAFPICILLN MRSGTSFSHG LIDFIVLSGN SNNFWLFPIV 370 380 390 400 410 420 GLFYGILYYG IFYFMIKKFN LKTPGREKSI TYINQKTIKE TALLVISILG GKTNIINLDA 430 440 450 460 470 CITRLRITVL DISKVNQKKL KNLGASGVIV SGSGIQIVFG TQSDHIKTEI DNYMSNTNQ

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27048.1.
RefSeq	NP_777943.1.
3D structure databases
SMR	Q89AG6. Positions 405-476.
ModBase	Search...
Genome annotation databases
GeneID	1058276.
GenomeReviews	Gene locus bbp_326 in contig AE016826_GR.
KEGG	bab:bbp326.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571563.
OMA	NNDGVAG.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_326-MONOMER.
Family and domain databases
InterPro	IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTGCB_BUCBP

Accession

Primary (citable) accession number: Q89AG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)