Q89AG2 (AMPA_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	bbp_332

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165732
Sites
	Active site	284	1	Potential
	Active site	358	1	Potential
	Metal binding	272	1	Manganese 2 By similarity
	Metal binding	277	1	Manganese 1 By similarity
	Metal binding	277	1	Manganese 2 By similarity
	Metal binding	295	1	Manganese 2 By similarity
	Metal binding	354	1	Manganese 1 By similarity
	Metal binding	356	1	Manganese 1 By similarity
	Metal binding	356	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AG2 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: F0628DD186BCB80E	FASTA	501	56,089
	10 20 30 40 50 60 MRYHISTNFF KKKYSDCLVF GIFDDLQLCE SVKVIDSGSD GYVSRLIKDN EISGQINESL 70 80 90 100 110 120 LLHSIPNFQK LKILFLGCGK KERFNINLYQ KLFKTSINII KKLSVKALIY FVTDFHIKNI 130 140 150 160 170 180 NTYWKIRHAV SEIQDNLYSF KNFKTSNNKN KFKISLEDIY FYLSDHDEIN SGNNAIQHGY 190 200 210 220 230 240 AISKGKKIAR DLSNMPPNIC NSSYLADQAM KLSQYYPDLI DVEIINDIDM NKLGMNAYLS 250 260 270 280 290 300 VGKGSKNKSL MSIIKYHGIS FSQCKNIILI GKGVTFDSGG ISIKTSRDLD EMKFDMSGAA 310 320 330 340 350 360 IVFGLMSIIS DLKLPLNIIG ILAGSENMVS SMSFRPGDIL TTMSGKTVEI LNTDAEGRLI 370 380 390 400 410 420 LCDVLTYVER FSPEVVIDIA TLTGACVVAL GHHTTGLLSN NDILAKDLQK ASKQTRDLIW 430 440 450 460 470 480 RMPLFEEYYK DLDSNVADMA NVGTNSAGMI TAACFLSKFS QKYAWAHLDV AGTAWISGKN 490 500 KGSTGRPINL LTQFLLNKLY K

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27053.1.
RefSeq	NP_777948.1. NC_004545.1.
3D structure databases
ProteinModelPortal	Q89AG2.
SMR	Q89AG2. Positions 1-498.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002255; EBBUCP00000002079; EBBUCG00000002255.
GeneID	1058145.
GenomeReviews	Gene locus bbp_332 in contig AE016826_GR.
KEGG	bab:bbp332.
PATRIC	21245453. VBIBucAph80364_0326.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008030.
HOGENOM	HBG742580.
OMA	NSGLMST.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_332-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BUCBP

Accession

Primary (citable) accession number: Q89AG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program