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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi486MagnesiumUniRule annotation1
Metal bindingi492MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:bbp_336
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000601 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001979121 – 693Polyribonucleotide nucleotidyltransferaseAdd BLAST693

Proteomic databases

PRIDEiQ89AF8.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi224915.bbp336.

Structurei

3D structure databases

ProteinModelPortaliQ89AF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini553 – 612KHUniRule annotationAdd BLAST60
Domaini622 – 690S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89AF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNPIVRKFQ YGQHIVTLET GIMARQATAA VLTSMDDTTV FVTVVGQKKL
60 70 80 90 100
QTEQKFFPMT VNYQERTYAA GRIPGGFFRR EGRPSENEIL IARLIDRPIR
110 120 130 140 150
PLFPKRFFNE VQIIATVVSL NPQVNPDIVA IIGASAALSI SGMPFLGPIG
160 170 180 190 200
AARIGYINNQ YILNPNVNEI KSSSLDLIIS GTEESVLMVE AEANMLTEEQ
210 220 230 240 250
IINAINYGHE QQKIVIKNIE HFAKKVKIPV WEQCLYPVND DLEKRIIEIS
260 270 280 290 300
EQEIVKAYNI FDKVIRLDVL DKIKSKAIEI IISEYSGIDE FEITSIISNI
310 320 330 340 350
ERKIVRNRVL KNRIRIDGRN KDEIRTLDVR TGVLPRVHGS ALFTRGETQS
360 370 380 390 400
LVSVTLGTSR DAQNLDELLG DKTDNFLFHY NFPPYAVGEI GMVGSPKRRE
410 420 430 440 450
IGHGKLAKRS ILAVMPKLDL FPYTIRIVSE ITESNGSSSM ASVCGASLAL
460 470 480 490 500
MDAGVPISNA IAGIAMGLIK EGDDFVVLTD ILGDEDHLGD MDFKISGSKK
510 520 530 540 550
GITALQMDIK VKGITNEIMK LALYQAKNAR IHILNIMKKS LNVPRKDISI
560 570 580 590 600
FAPRIHTIKI NPEKIKDVIG KGGSVIRMLT EETGTTIEIE DDGTVKISAV
610 620 630 640 650
MQEKAKCAIQ RIKEITAEVE VGSVYTGKVT RIVDFGAFVS IGIGKEGLVH
660 670 680 690
ISQIAHKRVD KVSDHLRLHQ EIFVKVLEVD RQGRLRLSIK GAK
Length:693
Mass (Da):77,000
Last modified:June 1, 2003 - v1
Checksum:i448E952C478C339B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO27057.1.
RefSeqiWP_011091458.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO27057; AAO27057; bbp_336.
KEGGibab:bbp_336.
PATRICi21245463. VBIBucAph80364_0331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO27057.1.
RefSeqiWP_011091458.1. NC_004545.1.

3D structure databases

ProteinModelPortaliQ89AF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224915.bbp336.

Proteomic databases

PRIDEiQ89AF8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO27057; AAO27057; bbp_336.
KEGGibab:bbp_336.
PATRICi21245463. VBIBucAph80364_0331.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_BUCBP
AccessioniPrimary (citable) accession number: Q89AF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.