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Q89AF8 (PNP_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:bbp_336
OrganismBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]
Taxonomic identifier224915 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length693 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595

Subunit structure

Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

mRNA catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5'-exoribonuclease activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

polyribonucleotide nucleotidyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 693693Polyribonucleotide nucleotidyltransferase HAMAP MF_01595
PRO_0000197912

Regions

Domain553 – 61260KH
Domain622 – 69069S1 motif

Sequences

Sequence LengthMass (Da)Tools
Q89AF8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 448E952C478C339B

FASTA69377,000
        10         20         30         40         50         60 
MLNPIVRKFQ YGQHIVTLET GIMARQATAA VLTSMDDTTV FVTVVGQKKL QTEQKFFPMT 

        70         80         90        100        110        120 
VNYQERTYAA GRIPGGFFRR EGRPSENEIL IARLIDRPIR PLFPKRFFNE VQIIATVVSL 

       130        140        150        160        170        180 
NPQVNPDIVA IIGASAALSI SGMPFLGPIG AARIGYINNQ YILNPNVNEI KSSSLDLIIS 

       190        200        210        220        230        240 
GTEESVLMVE AEANMLTEEQ IINAINYGHE QQKIVIKNIE HFAKKVKIPV WEQCLYPVND 

       250        260        270        280        290        300 
DLEKRIIEIS EQEIVKAYNI FDKVIRLDVL DKIKSKAIEI IISEYSGIDE FEITSIISNI 

       310        320        330        340        350        360 
ERKIVRNRVL KNRIRIDGRN KDEIRTLDVR TGVLPRVHGS ALFTRGETQS LVSVTLGTSR 

       370        380        390        400        410        420 
DAQNLDELLG DKTDNFLFHY NFPPYAVGEI GMVGSPKRRE IGHGKLAKRS ILAVMPKLDL 

       430        440        450        460        470        480 
FPYTIRIVSE ITESNGSSSM ASVCGASLAL MDAGVPISNA IAGIAMGLIK EGDDFVVLTD 

       490        500        510        520        530        540 
ILGDEDHLGD MDFKISGSKK GITALQMDIK VKGITNEIMK LALYQAKNAR IHILNIMKKS 

       550        560        570        580        590        600 
LNVPRKDISI FAPRIHTIKI NPEKIKDVIG KGGSVIRMLT EETGTTIEIE DDGTVKISAV 

       610        620        630        640        650        660 
MQEKAKCAIQ RIKEITAEVE VGSVYTGKVT RIVDFGAFVS IGIGKEGLVH ISQIAHKRVD 

       670        680        690 
KVSDHLRLHQ EIFVKVLEVD RQGRLRLSIK GAK

« Hide

References

[1]"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016826 Genomic DNA. Translation: AAO27057.1.
RefSeqNP_777952.1. NC_004545.1.

3D structure databases

ProteinModelPortalQ89AF8.
SMRQ89AF8. Positions 617-692.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBUCT00000002641; EBBUCP00000002465; EBBUCG00000002641.
GeneID1058421.
GenomeReviewsGene locus bbp_336 in contig AE016826_GR.
KEGGbab:bbp336.
PATRIC21245463. VBIBucAph80364_0331.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000007805.
HOGENOMHBG382411.
OMAYGETVVL.
PhylomeDBQ89AF8.
ProtClustDBPRK11824.

Enzyme and pathway databases

BioCycBAPH224915:BBP_336-MONOMER.

Family and domain databases

HAMAPMF_01595. PNPase.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR012162. PNPase.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
KOK00962.
PANTHERPTHR11252. PNPase. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF46915. 3_ExoRNase. 1 hit.
SSF55666. 3_ExoRNase. 2 hits.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMsTIGR03591. Polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_BUCBP
AccessionPrimary (citable) accession number: Q89AF8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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