Reviewed, UniProtKB/Swiss-Prot Q89AF8 (PNP_BUCBP)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	bbp_336

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000197912
Regions
	Domain	553 – 612	60	KH
	Domain	622 – 690	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q89AF8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 448E952C478C339B	FASTA	693	77,000
	10 20 30 40 50 60 MLNPIVRKFQ YGQHIVTLET GIMARQATAA VLTSMDDTTV FVTVVGQKKL QTEQKFFPMT 70 80 90 100 110 120 VNYQERTYAA GRIPGGFFRR EGRPSENEIL IARLIDRPIR PLFPKRFFNE VQIIATVVSL 130 140 150 160 170 180 NPQVNPDIVA IIGASAALSI SGMPFLGPIG AARIGYINNQ YILNPNVNEI KSSSLDLIIS 190 200 210 220 230 240 GTEESVLMVE AEANMLTEEQ IINAINYGHE QQKIVIKNIE HFAKKVKIPV WEQCLYPVND 250 260 270 280 290 300 DLEKRIIEIS EQEIVKAYNI FDKVIRLDVL DKIKSKAIEI IISEYSGIDE FEITSIISNI 310 320 330 340 350 360 ERKIVRNRVL KNRIRIDGRN KDEIRTLDVR TGVLPRVHGS ALFTRGETQS LVSVTLGTSR 370 380 390 400 410 420 DAQNLDELLG DKTDNFLFHY NFPPYAVGEI GMVGSPKRRE IGHGKLAKRS ILAVMPKLDL 430 440 450 460 470 480 FPYTIRIVSE ITESNGSSSM ASVCGASLAL MDAGVPISNA IAGIAMGLIK EGDDFVVLTD 490 500 510 520 530 540 ILGDEDHLGD MDFKISGSKK GITALQMDIK VKGITNEIMK LALYQAKNAR IHILNIMKKS 550 560 570 580 590 600 LNVPRKDISI FAPRIHTIKI NPEKIKDVIG KGGSVIRMLT EETGTTIEIE DDGTVKISAV 610 620 630 640 650 660 MQEKAKCAIQ RIKEITAEVE VGSVYTGKVT RIVDFGAFVS IGIGKEGLVH ISQIAHKRVD 670 680 690 KVSDHLRLHQ EIFVKVLEVD RQGRLRLSIK GAK

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO27057.1.
RefSeq	NP_777952.1.
3D structure databases
HSSP	HSSP built from PDB template 1SRO based on UniProtKB P05055.
SMR	Q89AF8. Positions 617-692.
ModBase	Search...
Genome annotation databases
GeneID	1058421.
GenomeReviews	Gene locus bbp_336 in contig AE016826_GR.
KEGG	bab:bbp336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AF8.
OMA	Q89AF8. GHGNLAK.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_336-MON.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_BUCBP

Accession

Primary (citable) accession number: Q89AF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)