Q89AD8 (GSH1_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	bbp_368

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Glutamate--cysteine ligase HAMAP MF_00578		PRO_0000192522

Sequences

	Sequence		Length	Mass (Da)
	Q89AD8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1FD19D58646C89BB	FASTA	521	61,433
	10 20 30 40 50 60 MIPKIYKKIK WLQSNPLILK KILRGIEREA LRTDIHGTII NTAYPQDKIG SALTHKWITT 70 80 90 100 110 120 DFSESLLEFI TPTNASTNYI LKFLNDTHKF VNDNLIQEYF WPFSIPPCKK YMHSIKLSKY 130 140 150 160 170 180 GTSNLGQVKT LYRKGLKHRY GILMNIISGV HYNFSLPRIF WNHWKKIHHK NTQYNTTSEG 190 200 210 220 230 240 YLCLIRNYYK FGWIIPYLFG ASPAVEPLFI KNKKHNYKFK KHHGMLYLPW STSLRLSDLG 250 260 270 280 290 300 HTNQSIKKLK LTFNSLSEYV LALEHGIKTP SKQFKNLGLY DQFGNFKQIN TNLLQTENEL 310 320 330 340 350 360 YTYIRPKQKL KNCESLSAAL RNRGIEYVEI RALDINPFTS TGVDKNQILL LDLFLIWCVL 370 380 390 400 410 420 ADSPKISSQE FHFFLKNWHT IITKGRKPKQ KININIYNTK NTIQTIGKTI LYDLFYIAEI 430 440 450 460 470 480 LDSLSNNNNY QETCKNLILY FDYPELTYSE KLLNKFMCYG IYETGANLFV KYKQKLHNDS 490 500 510 520 FKILSKKDLN NEMMKSNNSQ KLIEKQDTLN FKEYLNLYYT K

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27085.1.
RefSeq	NP_777980.1. NC_004545.1.
3D structure databases
ProteinModelPortal	Q89AD8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002493; EBBUCP00000002317; EBBUCG00000002493.
GeneID	1058287.
GenomeReviews	Gene locus bbp_368 in contig AE016826_GR.
KEGG	bab:bbp368.
PATRIC	21245529. VBIBucAph80364_0361.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007855.
HOGENOM	HBG289023.
OMA	FGLIRNY.
ProtClustDB	PRK02107.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_368-MONOMER.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
KO	K01919.
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. Glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_BUCBP

Accession

Primary (citable) accession number: Q89AD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program