Reviewed, UniProtKB/Swiss-Prot Q89AB8 (DHAS_BUCBP)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	bbp_398

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Aspartate-semialdehyde dehydrogenase		PRO_0000141365
Sites
	Active site	137	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89AB8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8DDBA2BADF6C7516	FASTA	369	41,494
	10 20 30 40 50 60 MKKNIGLIGW RGMVGSVLVE RMICENDFFH FNPIFFSTSQ KNSKGPTING EYFGVLKDAY 70 80 90 100 110 120 DLDILKELDI LISCQGSDYT NKVYFKLRDI GWNGYWIDAA SVLRMNQDTV IILDPVNSNT 130 140 150 160 170 180 IRESIDSGFK TFVGGNCTVS LMLMSLGGLF SEKLIEWITV STYQAASGSG AKYIEELLMQ 190 200 210 220 230 240 MGSMYTEASG YLGNPAHSIL NIEKKVRNLS CSDKFPKKNF KVPLAGSLIP WIDKKMRNGQ 250 260 270 280 290 300 SREEWKIQAE TNKILSSNKD ILIDGLCVRI GALRCHSQSF VIKLKKDISI PEIEQILLNH 310 320 330 340 350 360 NSWTKVIPNR KHDTMTELTP AAVTGTLNTP VGRLRKLNIG QKYLSAFTVG DQLLWGASEP LRRMLKFLI

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO27110.1.
RefSeq	NP_778005.1.
3D structure databases
HSSP	HSSP built from PDB template 1BRM based on UniProtKB P00353.
ModBase	Search...
Genome annotation databases
GeneID	1058293.
GenomeReviews	Gene locus bbp_398 in contig AE016826_GR.
KEGG	bab:bbp398.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89AB8.
OMA	Q89AB8. IDGLCVR.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_398-MON.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR011534. Asp_ADH_proteob. IPR012080. Asp_semialdehyde_DH. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01745. asd_gamma. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_BUCBP

Accession

Primary (citable) accession number: Q89AB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)